Detailed information of accessory protein
Summary
| Accessory protein ID | ACP26818  |
| Type | adaptor |
| Related T6SS (Type) | T6SS00050 (Type i1) |
| Strain | Photorhabdus laumondii subsp. laumondii TTO1 |
| Replicon | chromosome |
| Related structure protein | - |
| Related effector | Plu0353 (Tre23) |
| Description | A Strep-tag pull-down experiment was performed from E. coli cells producing full-length FLAG- tagged Rhs, His-tagged VgrG and the Strep-tagged EagR chaperone and showed EagR is the bridge of VgrG and Rhs effector. |
| Reference | [1] Jurėnas D, Payelleville A, Roghanian M, et al. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 2021 Aug 20;49(14):8384-8395. doi: 10.1093/nar/gkab608. PMID: 34255843 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | Plu0354 (EagR) |
| Coordinates (Strand) | 372357..372677 (-) |
| NCBI ID | WP_011144744.1 |
| RefSeq | NC_005126 |
| Uniprot ID | A0A6L9JXU2_PHOLM, Q7N9I1_PHOLL |
| KEGG ID | plu:plu0352, plum:A4R40_01715 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-106 | MKHESIQLAREEVNAIIKLILYIKFECEDPGTLIYSSSPLINSALEKMLNMYGYKDDWDKVFSKFLEADKNFVIKRVEYLEKHENSPLDEGIKQQILSNHAYPYKW |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9939 | - | - |
Protein sequence: 107 a.a.
>ACP26818 NC_005126:c372677-372357 [Photorhabdus laumondii subsp. laumondii TTO1]
MKHESIQLAREEVNAIIKLILYIKFECEDPGTLIYSSSPLINSALEKMLNMYGYKDDWDKVFSKFLEADKNFVIKRVEYL
EKHENSPLDEGIKQQILSNHAYPYKW*
MKHESIQLAREEVNAIIKLILYIKFECEDPGTLIYSSSPLINSALEKMLNMYGYKDDWDKVFSKFLEADKNFVIKRVEYL
EKHENSPLDEGIKQQILSNHAYPYKW*
Nucleotide sequence: 321 bp
>ACP26818 NC_005126:c372677-372357 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGAAGCACGAATCTATTCAATTAGCAAGGGAAGAGGTGAATGCAATAATCAAGCTGATCCTTTACATAAAGTTTGAATG
TGAAGATCCTGGAACATTGATATATTCAAGTAGTCCTTTGATAAATTCAGCATTGGAAAAAATGTTGAATATGTATGGTT
ATAAGGATGATTGGGATAAAGTTTTTTCAAAATTTCTAGAAGCAGATAAGAATTTTGTAATCAAAAGAGTCGAATATTTA
GAAAAACATGAAAATAGTCCTCTTGATGAGGGAATTAAGCAGCAAATCTTATCAAATCATGCATATCCATATAAGTGGTA
G
ATGAAGCACGAATCTATTCAATTAGCAAGGGAAGAGGTGAATGCAATAATCAAGCTGATCCTTTACATAAAGTTTGAATG
TGAAGATCCTGGAACATTGATATATTCAAGTAGTCCTTTGATAAATTCAGCATTGGAAAAAATGTTGAATATGTATGGTT
ATAAGGATGATTGGGATAAAGTTTTTTCAAAATTTCTAGAAGCAGATAAGAATTTTGTAATCAAAAGAGTCGAATATTTA
GAAAAACATGAAAATAGTCCTCTTGATGAGGGAATTAAGCAGCAAATCTTATCAAATCATGCATATCCATATAAGTGGTA
G