Detailed information of accessory protein


Summary

Accessory protein ID ACP00027 experimental
Type chaperone
Related T6SS (Type) T6SS00838 (Type i1)
Strain Proteus mirabilis BB2000
Replicon chromosome
Related structure protein -
Related effector BB2000_3005 (IdsD)
Description DUF4123
Reference [1] Zepeda-Rivera MA, Saak CC, Gibbs KA. A Proposed Chaperone of the Bacterial Type VI Secretion System Functions To Constrain a Self-Identity Protein. J Bacteriol. 2018 Jun 25;200(14). pii: e00688-17. doi: 10.1128/JB.00688-17. Print 2018 Jul 15. PMID: 29555703
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) BB2000_RS14605 (idsC)
Coordinates (Strand) 3278448..3279671 (+)
NCBI ID 529249287
RefSeq NC_022000
Uniprot ID B4YPP3_PROMI
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF4123 PF13503.8 7.9e-11 128..253
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-407 MLLSPNPLYKAYWVAQCRYTRNGEQFKGVMTVAGTSQSQAIKQMRQYFTAHPGEYTFADYDTLIPLITHIEQSSTLELPLIRQVREQHNAKVSAVLVDKCNLTHPRPSEKGDIHYREGQPTFIEYSHLYVVIDSGEYHRQTGQHLVPKLHGSQLPWKSLYQGETQDSLEDKAPYLVHIAANQAGQRFLAHYLNLPHKASLGLFINSLKPFTDIHRQMRKLTYLYNQKLESWNFFRFYDVKHFIPFIESLTHGQLINVANGVNAFYGYSAQYPDGVEITFHPDYLYDGSKREPLFINTYLYNHYANITQMQTVAKAKALIEQFSQVEGDELEGDALMGYCIHAANCSFLDDIHQSKALLYDLQARYLCRHQPRTWQIANEKAAPYKYNQVLLSYHRYIACLNTQGEMK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9485 - -


  Protein sequence: 408 a.a.    

>ACP00027 NC_022000:3278448-3279671 [Proteus mirabilis BB2000]
MLLSPNPLYKAYWVAQCRYTRNGEQFKGVMTVAGTSQSQAIKQMRQYFTAHPGEYTFADYDTLIPLITHIEQSSTLELPL
IRQVREQHNAKVSAVLVDKCNLTHPRPSEKGDIHYREGQPTFIEYSHLYVVIDSGEYHRQTGQHLVPKLHGSQLPWKSLY
QGETQDSLEDKAPYLVHIAANQAGQRFLAHYLNLPHKASLGLFINSLKPFTDIHRQMRKLTYLYNQKLESWNFFRFYDVK
HFIPFIESLTHGQLINVANGVNAFYGYSAQYPDGVEITFHPDYLYDGSKREPLFINTYLYNHYANITQMQTVAKAKALIE
QFSQVEGDELEGDALMGYCIHAANCSFLDDIHQSKALLYDLQARYLCRHQPRTWQIANEKAAPYKYNQVLLSYHRYIACL
NTQGEMK*

  Nucleotide sequence: 1224 bp    

>ACP00027 NC_022000:3278448-3279671 [Proteus mirabilis BB2000]
ATGCTCTTGAGTCCAAATCCCCTCTATAAAGCGTATTGGGTTGCTCAATGCCGTTATACTCGCAACGGTGAACAATTCAA
GGGGGTGATGACCGTAGCAGGTACAAGTCAATCACAAGCTATTAAGCAGATGCGCCAGTACTTTACGGCTCACCCAGGTG
AATATACCTTTGCGGACTATGACACATTAATCCCTTTAATCACCCATATTGAACAAAGTTCAACCTTAGAATTACCGTTA
ATACGGCAAGTACGTGAGCAACATAATGCAAAGGTTTCAGCCGTATTAGTGGATAAATGCAACCTCACACACCCAAGACC
GTCAGAAAAAGGCGACATTCATTACCGTGAGGGGCAACCTACGTTTATTGAATATTCGCATCTCTATGTCGTCATTGACA
GTGGGGAATACCACCGCCAAACCGGGCAACATCTTGTACCGAAACTGCATGGCTCACAACTGCCATGGAAATCACTCTAT
CAAGGAGAAACCCAAGACAGCCTTGAAGATAAAGCCCCTTATTTGGTACACATTGCCGCCAATCAAGCCGGTCAGCGGTT
TCTGGCTCATTACTTGAATTTACCACATAAAGCGAGTCTCGGATTATTTATCAATAGCCTCAAACCCTTTACCGATATTC
ACCGGCAAATGCGAAAACTCACCTATTTATATAATCAAAAACTGGAGAGTTGGAATTTCTTTCGTTTTTATGATGTTAAG
CACTTTATCCCATTTATTGAGTCTTTGACTCACGGACAGTTAATTAATGTGGCCAATGGGGTAAATGCGTTCTACGGCTA
TAGTGCACAATACCCCGATGGGGTTGAAATCACCTTTCACCCAGATTATCTGTATGACGGCAGTAAGCGAGAGCCGTTAT
TTATTAATACCTATTTATACAATCACTACGCGAATATCACACAGATGCAAACTGTGGCTAAAGCTAAGGCACTGATTGAA
CAATTTTCTCAGGTAGAAGGGGATGAGTTAGAGGGTGACGCATTAATGGGCTACTGTATACACGCAGCAAATTGCAGTTT
TTTAGACGATATTCATCAATCAAAAGCGTTATTGTACGATTTGCAAGCTCGCTATTTGTGCCGTCATCAACCGAGAACAT
GGCAGATCGCCAATGAAAAAGCTGCACCTTATAAATACAACCAAGTTTTATTGAGTTACCACCGTTATATCGCCTGCTTA
AATACCCAAGGAGAAATGAAATGA