SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01125
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00838 (Type i1)
Strain	Proteus mirabilis BB2000
Replicon	chromosome
Sequence	Protein sequence (1035 a.a.); Nucleotide sequence (3105 bp)
Description	-
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	BB2000_RS14610
Coordinates (Strand)	3279668..3282772 (+)
NCBI ID	529238655
RefSeq	NC_022000
Uniprot ID	UPI00017A2F79
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-692	MTGEVNEKYLTPQERKARQMVKAVNEASPRNLPADAVVCPCENEHRPVYPVRYAYTNFYCDLHFSTIEQAPNKTLEASIPPSINQLLNAKDVTASKGFSARLLRQGWVYVFEEGNYPTRSNSSNKSYQEQNVDATKGRLLVFQHQVTTSDGNENFIPYIFKQLKNGGVTLKKNGNSNPYLAIPKDVKEATILFSESKLSDYTLKKIISSSKFRSKLMQKINFIDYNNNDYCIELNKDNLNRLVEDYKEEVDKFKLFVKEFTHSNIPSSFFSDTTKIPDLPQDATVLINQVNSVLDYNEKATLLILKDPVGYQKDILSYYNIVTKLHLLYQNYYSHPDKIGQFITSIQEASHHIKDTDEKEKMQTILKESINQNALDNEWKNIHKTFIFFEKHQRIVLSLYESFMNNPAIINENGGLKHYFDYAFSYHERITKEDVFSIDFFKDLNQAFDLYFDLVSPLMNSIEGQRTLDKLYSINDEENNSLWVGVTKKVISLIANSKIKDALLNVQEYAMHIENFVNKLAFICSDSIGFAFTKTSKVLSHYDIKNRLINTKGIDYLAQKILPMILAFCNTKISLTEFVKLSGNELNQWMEQLRKLTGQIVPNLQHPKLNKLFSWKQKIINLGEETAVLIPKIEIIDITKNKIYIYGKDALQVSTKLFLNGFSMITGSIQAYTLQGMSLYERNDPLKLSPYN
Transmembrane helix	693-710	LYTAQIIANLFVASYSIL
Inside	711-782	KVSQEATKLSQTVSSTTLKFFLDKIKLPMLTTEVGTKRMAALGKIAGAVGAALAARDALEAFHIGNYKQSVS
Transmembrane helix	783-805	NIAIVIGSIILITAVTGGWALFA
Outside	806-1034	GALILGGFISSQLTSWSHLETLLKHSFWGNEKRSNFWDNDRPTPIGEQLKQYIKEFEFYKQKGLIELQEFYNLFYTAKMTQEKIPNGKLRLSFEFTNFTPGISEVYFHFVTEVGYHSGLAEEIKTPSSAYVLNKRKDLLEISEQLKMASEKGDWNPETGIFKFSLEVQSQLVNTYSAFGAHPNSRIGIEDLYWYYQVNPEVTTPMRYINWGGDTQENNQLLGFINSENI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9583	-	-

Protein sequence: 1035 a.a. .

                                    >EFF01125 NC_022000:3279668-3282772  (Unknown) [Proteus mirabilis BB2000]
MTGEVNEKYLTPQERKARQMVKAVNEASPRNLPADAVVCPCENEHRPVYPVRYAYTNFYCDLHFSTIEQAPNKTLEASIP
PSINQLLNAKDVTASKGFSARLLRQGWVYVFEEGNYPTRSNSSNKSYQEQNVDATKGRLLVFQHQVTTSDGNENFIPYIF
KQLKNGGVTLKKNGNSNPYLAIPKDVKEATILFSESKLSDYTLKKIISSSKFRSKLMQKINFIDYNNNDYCIELNKDNLN
RLVEDYKEEVDKFKLFVKEFTHSNIPSSFFSDTTKIPDLPQDATVLINQVNSVLDYNEKATLLILKDPVGYQKDILSYYN
IVTKLHLLYQNYYSHPDKIGQFITSIQEASHHIKDTDEKEKMQTILKESINQNALDNEWKNIHKTFIFFEKHQRIVLSLY
ESFMNNPAIINENGGLKHYFDYAFSYHERITKEDVFSIDFFKDLNQAFDLYFDLVSPLMNSIEGQRTLDKLYSINDEENN
SLWVGVTKKVISLIANSKIKDALLNVQEYAMHIENFVNKLAFICSDSIGFAFTKTSKVLSHYDIKNRLINTKGIDYLAQK
ILPMILAFCNTKISLTEFVKLSGNELNQWMEQLRKLTGQIVPNLQHPKLNKLFSWKQKIINLGEETAVLIPKIEIIDITK
NKIYIYGKDALQVSTKLFLNGFSMITGSIQAYTLQGMSLYERNDPLKLSPYNLYTAQIIANLFVASYSILKVSQEATKLS
QTVSSTTLKFFLDKIKLPMLTTEVGTKRMAALGKIAGAVGAALAARDALEAFHIGNYKQSVSNIAIVIGSIILITAVTGG
WALFAGALILGGFISSQLTSWSHLETLLKHSFWGNEKRSNFWDNDRPTPIGEQLKQYIKEFEFYKQKGLIELQEFYNLFY
TAKMTQEKIPNGKLRLSFEFTNFTPGISEVYFHFVTEVGYHSGLAEEIKTPSSAYVLNKRKDLLEISEQLKMASEKGDWN
PETGIFKFSLEVQSQLVNTYSAFGAHPNSRIGIEDLYWYYQVNPEVTTPMRYINWGGDTQENNQLLGFINSENI*

                                

Nucleotide sequence: 3105 bp .

                                    >EFF01125 NC_022000:3279668-3282772  (Unknown) [Proteus mirabilis BB2000]
ATGACTGGAGAAGTGAATGAGAAATATTTAACACCGCAAGAGCGCAAAGCGCGTCAGATGGTGAAGGCGGTAAACGAAGC
GAGCCCACGAAACTTACCGGCCGACGCGGTGGTATGCCCATGTGAAAATGAACATCGCCCTGTTTATCCGGTGCGTTATG
CATATACCAACTTTTATTGTGATTTACATTTTTCTACAATTGAACAAGCACCAAATAAAACGTTAGAAGCGAGTATTCCT
CCTTCTATTAATCAATTATTGAATGCGAAAGATGTTACTGCTAGTAAAGGATTTTCTGCAAGATTATTAAGACAAGGTTG
GGTTTATGTTTTTGAAGAAGGCAATTACCCTACTAGAAGTAATTCTAGCAATAAAAGTTATCAAGAACAAAATGTTGATG
CAACAAAAGGACGCCTATTAGTTTTTCAACATCAAGTGACAACCAGTGATGGCAATGAAAATTTCATTCCATATATATTT
AAGCAATTAAAAAATGGGGGTGTCACTTTAAAGAAAAACGGAAATAGTAATCCTTATTTAGCTATACCGAAAGATGTGAA
GGAAGCGACTATCTTATTCAGCGAAAGTAAATTATCTGATTACACACTTAAAAAAATCATTTCATCTTCTAAGTTTAGAT
CGAAATTAATGCAAAAAATCAATTTTATTGATTACAATAATAACGATTATTGTATTGAGCTAAATAAAGATAATTTAAAT
CGACTTGTTGAGGATTATAAAGAAGAAGTTGATAAATTTAAGCTATTTGTTAAAGAATTCACGCATTCAAATATACCCTC
TTCTTTTTTTTCTGATACCACAAAAATACCCGACTTACCACAAGATGCAACTGTTTTGATTAATCAAGTAAATAGTGTTC
TAGATTATAATGAAAAAGCGACATTGCTTATTTTAAAAGATCCCGTAGGATACCAAAAAGATATTTTATCTTATTATAAT
ATTGTAACAAAATTACATTTATTATATCAGAACTATTATAGCCATCCGGATAAAATTGGCCAATTTATTACGAGTATACA
AGAAGCTAGTCATCACATTAAAGATACTGATGAAAAAGAAAAAATGCAAACAATACTAAAAGAGAGTATTAATCAGAATG
CATTAGATAATGAGTGGAAAAATATTCATAAAACATTTATTTTTTTTGAGAAACATCAACGTATTGTATTATCATTGTAT
GAAAGCTTTATGAATAATCCGGCAATCATTAATGAAAACGGTGGCTTAAAACATTATTTTGATTATGCTTTTTCATATCA
CGAACGTATAACTAAAGAAGATGTCTTTAGTATTGATTTTTTTAAGGACCTTAATCAAGCTTTTGATTTATATTTTGATT
TAGTATCTCCATTAATGAATTCTATCGAAGGACAAAGAACATTAGATAAATTATATTCAATTAATGATGAAGAAAATAAT
AGCCTGTGGGTGGGAGTGACAAAGAAAGTAATTAGTCTTATTGCTAATTCAAAAATTAAGGACGCTCTTTTAAATGTACA
AGAATATGCAATGCATATCGAAAATTTTGTTAATAAGCTTGCATTTATTTGCTCAGATAGCATTGGTTTCGCGTTTACAA
AAACAAGTAAGGTACTTTCTCATTACGATATTAAAAATAGACTAATTAATACTAAGGGTATTGACTATTTAGCCCAAAAG
ATACTTCCGATGATACTGGCATTTTGCAACACAAAAATTTCATTAACCGAGTTTGTTAAATTATCGGGTAATGAGCTTAA
CCAGTGGATGGAACAACTCCGTAAATTAACGGGACAAATAGTACCAAATCTGCAACATCCTAAATTAAATAAGCTTTTTT
CTTGGAAACAAAAAATAATAAATCTAGGCGAAGAGACTGCCGTTCTTATTCCGAAGATTGAGATCATAGATATTACTAAA
AATAAGATTTATATTTATGGCAAAGATGCATTACAGGTTTCCACTAAGCTATTTTTAAACGGTTTCAGCATGATCACCGG
ATCAATCCAAGCTTATACATTACAAGGTATGAGTTTGTATGAACGTAATGACCCATTAAAACTATCCCCCTATAATCTGT
ATACAGCACAGATTATTGCGAATTTATTTGTAGCAAGCTACAGTATTTTAAAAGTTTCTCAAGAGGCGACAAAATTATCT
CAAACAGTATCGAGCACCACACTGAAATTCTTTTTAGATAAAATAAAATTACCTATGCTAACAACAGAAGTGGGAACCAA
AAGAATGGCAGCATTAGGTAAGATTGCAGGTGCCGTTGGTGCCGCTCTTGCCGCTCGTGACGCATTAGAAGCTTTTCATA
TTGGAAATTATAAACAATCTGTATCAAATATAGCTATTGTGATTGGTTCTATAATTTTAATTACTGCTGTTACTGGAGGA
TGGGCTTTATTTGCTGGGGCACTTATTTTGGGGGGATTTATCTCAAGCCAACTCACCAGTTGGAGTCATTTAGAAACTTT
ACTAAAACATAGTTTTTGGGGGAATGAAAAAAGGTCTAATTTTTGGGATAATGATAGACCAACACCGATAGGAGAACAAT
TAAAACAATATATAAAAGAATTTGAATTCTATAAACAAAAAGGGCTAATTGAATTACAAGAGTTTTATAATCTATTTTAT
ACAGCTAAAATGACTCAAGAAAAAATACCAAATGGAAAACTCCGCTTATCTTTTGAATTTACTAATTTTACCCCAGGGAT
TTCAGAAGTATATTTTCACTTTGTTACAGAGGTTGGTTATCACAGCGGCTTGGCAGAAGAAATAAAAACACCTAGTTCAG
CTTATGTTCTAAATAAACGAAAAGACCTCTTAGAAATTAGCGAACAATTAAAAATGGCAAGTGAAAAAGGTGATTGGAAC
CCTGAAACAGGTATATTTAAATTTAGTTTGGAAGTACAGTCTCAATTAGTAAATACATATTCTGCTTTTGGTGCACATCC
TAATAGCCGTATAGGTATTGAAGATTTATATTGGTATTATCAAGTCAATCCCGAGGTAACAACACCGATGCGTTATATCA
ATTGGGGGGGAGATACCCAAGAAAACAATCAGCTTTTAGGCTTTATTAACAGTGAGAATATCTAA

                                