Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   LLNCDO184_RS05405 Genome accession   NZ_CP155124
Coordinates   980390..981238 (+) Length   282 a.a.
NCBI ID   WP_012897825.1    Uniprot ID   A0A2A9I6Y4
Organism   Lactococcus lactis subsp. lactis strain NCDO184     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 975390..986238
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLNCDO184_RS05380 (LLNCDO184_05370) ilvN 976145..976621 (+) 477 WP_063283635.1 acetolactate synthase small subunit -
  LLNCDO184_RS05385 (LLNCDO184_05375) ilvC 976667..977688 (+) 1022 Protein_1040 ketol-acid reductoisomerase -
  LLNCDO184_RS05390 (LLNCDO184_05380) ilvA 977744..978994 (+) 1251 Protein_1041 threonine ammonia-lyase IlvA -
  LLNCDO184_RS05395 (LLNCDO184_05385) budA 979113..979823 (+) 711 WP_003131140.1 acetolactate decarboxylase -
  LLNCDO184_RS05400 (LLNCDO184_05390) - 979864..980244 (+) 381 WP_063283634.1 RidA family protein -
  LLNCDO184_RS05405 (LLNCDO184_05395) dprA 980390..981238 (+) 849 WP_012897825.1 DNA-processing protein DprA Machinery gene
  LLNCDO184_RS05410 (LLNCDO184_05400) topA 981500..983632 (+) 2133 WP_063283633.1 type I DNA topoisomerase -
  LLNCDO184_RS05415 (LLNCDO184_05405) trmFO 983704..985047 (+) 1344 WP_063283632.1 methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO -
  LLNCDO184_RS05420 (LLNCDO184_05410) xerS 985092..986162 (-) 1071 WP_063283631.1 tyrosine recombinase XerS -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 32119.17 Da        Isoelectric Point: 9.5086

>NTDB_id=996262 LLNCDO184_RS05405 WP_012897825.1 980390..981238(+) (dprA) [Lactococcus lactis subsp. lactis strain NCDO184]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDVSKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide


Download         Length: 849 bp        

>NTDB_id=996262 LLNCDO184_RS05405 WP_012897825.1 980390..981238(+) (dprA) [Lactococcus lactis subsp. lactis strain NCDO184]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGACGTAAGTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGGCTCAAGGAAATTTACAATCCACCGGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGTGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTCTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAATATCTTGCTAAAAACCA
ACTCATACTATCAGAATATTCAGTCGGAGAAAAACCACTTCGCTATCATTTTCCTGAACGCAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAACGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATACAACAAGGAGCAAA
ATTAATCTATCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(2-63)

DNA recombination-mediator protein A (DNA_processg_A)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A9I6Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Lactococcus lactis subsp. cremoris KW2

86.879

100

0.869
  dprA Streptococcus mutans UA159

60.072

98.582

0.592
  dprA/cilB/dalA Streptococcus mitis SK321

54.643

99.291

0.543
  dprA/cilB/dalA Streptococcus pneumoniae R6

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

53.929

99.291

0.535
  dprA/cilB/dalA Streptococcus pneumoniae D39

53.929

99.291

0.535
  dprA Legionella pneumophila strain ERS1305867

39.13

97.872

0.383
  dprA Haemophilus influenzae Rd KW20

38.889

95.745

0.372
  dprA Bacillus subtilis subsp. subtilis str. 168

43.777

82.624

0.362
  dprA Latilactobacillus sakei subsp. sakei 23K

40

90.426

0.362

Multiple sequence alignment