Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   AAYT23_RS09285 Genome accession   NZ_CP154877
Coordinates   1794420..1795436 (-) Length   338 a.a.
NCBI ID   WP_075218762.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 20234295     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1789420..1800436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAYT23_RS09240 (AAYT23_09225) - 1789497..1789628 (-) 132 WP_000768904.1 hypothetical protein -
  AAYT23_RS09245 (AAYT23_09230) - 1789629..1790819 (-) 1191 WP_000167753.1 acetate kinase -
  AAYT23_RS09250 (AAYT23_09235) comYH 1790870..1791823 (-) 954 WP_000345126.1 class I SAM-dependent methyltransferase Machinery gene
  AAYT23_RS09255 (AAYT23_09240) - 1791884..1792478 (-) 595 Protein_1795 class I SAM-dependent methyltransferase -
  AAYT23_RS09260 (AAYT23_09245) comGG/cglG 1792615..1793028 (-) 414 WP_000265620.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAYT23_RS09265 (AAYT23_09250) comGF/cglF 1793006..1793467 (-) 462 WP_000250540.1 competence type IV pilus minor pilin ComGF Machinery gene
  AAYT23_RS09270 (AAYT23_09255) comGE/cglE 1793430..1793732 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAYT23_RS09275 (AAYT23_09260) comGD/cglD 1793695..1794099 (-) 405 WP_000588023.1 competence type IV pilus minor pilin ComGD Machinery gene
  AAYT23_RS09280 (AAYT23_09265) comGC/cglC 1794092..1794418 (-) 327 WP_000738631.1 comG operon protein ComGC Machinery gene
  AAYT23_RS09285 (AAYT23_09270) comGB/cglB 1794420..1795436 (-) 1017 WP_075218762.1 competence type IV pilus assembly protein ComGB Machinery gene
  AAYT23_RS09290 (AAYT23_09275) comGA/cglA/cilD 1795384..1796325 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  AAYT23_RS09295 (AAYT23_09280) - 1796401..1796766 (-) 366 WP_000286415.1 DUF1033 family protein -
  AAYT23_RS09300 (AAYT23_09285) - 1796917..1797975 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  AAYT23_RS09305 (AAYT23_09290) nagA 1798138..1799289 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38478.51 Da        Isoelectric Point: 9.6073

>NTDB_id=994485 AAYT23_RS09285 WP_075218762.1 1794420..1795436(-) (comGB/cglB) [Streptococcus pneumoniae strain 20234295]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVIQMRAGLSQGKSFSEMMESLGFSSTI
ITQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATQIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=994485 AAYT23_RS09285 WP_075218762.1 1794420..1795436(-) (comGB/cglB) [Streptococcus pneumoniae strain 20234295]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TGATCCAGATGCGTGCGGGCTTGTCTCAAGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATTTTCAAGCACCATT
ATCACTCAGTTATCCCTAGCTGAAGTTCATGGAAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCTAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCAAATTATCGGTAATCTGCCCCAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGTTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.675

100

0.947
  comYB Streptococcus gordonii str. Challis substr. CH1

71.131

99.408

0.707
  comYB Streptococcus mutans UA140

57.862

94.083

0.544
  comYB Streptococcus mutans UA159

57.862

94.083

0.544
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment