Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AAIA72_RS08150 Genome accession   NZ_CP154858
Coordinates   1665235..1667232 (-) Length   665 a.a.
NCBI ID   WP_369602903.1    Uniprot ID   A0AB39UZY3
Organism   Hahella sp. SMD15-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1660235..1672232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAIA72_RS08135 (AAIA72_08125) - 1661828..1663492 (-) 1665 WP_369602900.1 AAA family ATPase -
  AAIA72_RS08140 (AAIA72_08130) aroB 1663489..1664607 (-) 1119 WP_369602901.1 3-dehydroquinate synthase -
  AAIA72_RS08145 (AAIA72_08135) aroK 1664600..1665130 (-) 531 WP_369602902.1 shikimate kinase AroK -
  AAIA72_RS08150 (AAIA72_08140) pilQ 1665235..1667232 (-) 1998 WP_369602903.1 type IV pilus secretin PilQ Machinery gene
  AAIA72_RS08155 (AAIA72_08145) - 1667376..1667915 (-) 540 WP_369602904.1 pilus assembly protein PilP -
  AAIA72_RS08160 (AAIA72_08150) - 1667912..1668556 (-) 645 WP_369602905.1 type 4a pilus biogenesis protein PilO -
  AAIA72_RS08165 (AAIA72_08155) - 1668569..1669147 (-) 579 WP_369602906.1 PilN domain-containing protein -
  AAIA72_RS08170 (AAIA72_08160) pilM 1669147..1670211 (-) 1065 WP_369602907.1 pilus assembly protein PilM Machinery gene
  AAIA72_RS08175 (AAIA72_08165) - 1670383..1671605 (+) 1223 Protein_1585 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 665 a.a.        Molecular weight: 72322.84 Da        Isoelectric Point: 4.7880

>NTDB_id=994130 AAIA72_RS08150 WP_369602903.1 1665235..1667232(-) (pilQ) [Hahella sp. SMD15-11]
MPGDRTEIKLSFDGTPPQPKGYAIEQPARIALDLPGVSSKLSERYHQLSTGNTQSVTVLEASGRTRVIVNLLSLVPYATR
VDGNALIVQLGAKPGAEAQTSTASAATVSMPVATASADAAIANVDFRRGERGDGRVVIELSNPKIAVDMSEESGRIKLVF
NDAKLPTELRRRLDVIDFATPVTLIDAYEEEGNAVIAVKAQGEYDYLAYQTENVFTLAVEPLTADEAEQRKKEKFPYTGE
KLSLNFQDIEVRSVLQLIADFTGLNLVASDTVGGRITLRLQNVPWDQALELVLKTKGLDKRKVGNVLLVAPAEEIAARER
LELEANKQVRELAPVRLEVIQINYAKAADLVELLKQDKDLISERGFISSDARTNTISIRETAEKIAQVRKLIQTWDVPVR
QVLIEARIVRARTDVTSDLGVQWGGGHKAVRGRNVYYVGGSNNQVQQQANNNGSPNAGDQDFVSNNYAFPDAYAVDLAVT
KANTSSLALGFARDNLLLNLELSALEADGKGEIVAQPKVVTADRQTALIESGEEIPYQEASSSGATSVSFKKAVLSLEVT
PQITPDDKIIMDLRVNQDSRGEVTAGIPSIDTNEVKTQVLVGNGETVVLGGIFTSEETNTVTKTPFLGDIPYLGRLFTKK
ESNNQRSELLIFITPKLLKNGLVEQ

Nucleotide


Download         Length: 1998 bp        

>NTDB_id=994130 AAIA72_RS08150 WP_369602903.1 1665235..1667232(-) (pilQ) [Hahella sp. SMD15-11]
TTGCCCGGTGACCGGACCGAGATCAAGCTCAGCTTTGACGGGACACCGCCACAGCCAAAAGGGTATGCCATTGAGCAGCC
TGCACGGATTGCACTGGATCTGCCGGGCGTTTCAAGCAAGCTGAGTGAGCGTTACCATCAGCTCTCCACAGGGAATACTC
AGAGCGTCACGGTTCTCGAGGCTTCAGGTCGGACGCGTGTTATTGTCAATCTTCTGTCTCTGGTACCTTATGCCACCCGC
GTGGATGGCAACGCGCTCATCGTCCAGTTGGGGGCCAAGCCCGGTGCGGAAGCACAAACCAGCACTGCCAGCGCGGCGAC
CGTTTCCATGCCTGTCGCGACGGCCAGTGCAGATGCTGCCATTGCGAATGTGGATTTCCGCCGGGGTGAACGCGGAGATG
GTCGTGTGGTCATCGAGCTGTCCAATCCCAAAATCGCGGTGGACATGAGCGAAGAGTCAGGACGGATCAAACTGGTCTTT
AATGATGCCAAGCTGCCGACCGAGTTGCGTCGTCGTCTGGATGTCATTGATTTTGCCACGCCTGTCACCCTGATTGATGC
GTATGAAGAAGAAGGCAACGCGGTGATTGCGGTCAAGGCACAGGGTGAGTATGACTATCTGGCGTACCAGACAGAAAATG
TCTTCACGCTGGCAGTGGAACCCCTGACTGCCGACGAGGCCGAGCAGCGCAAGAAGGAAAAATTCCCCTATACCGGCGAG
AAGCTGTCCCTCAACTTCCAGGATATCGAGGTGCGGAGCGTATTGCAGCTGATTGCCGACTTTACCGGCCTGAACCTTGT
GGCCAGTGATACTGTAGGAGGCCGCATTACACTGCGTCTGCAGAATGTGCCCTGGGATCAGGCGCTCGAGCTGGTGCTGA
AGACCAAGGGCCTGGACAAGCGCAAGGTCGGAAACGTCCTGCTGGTCGCGCCGGCCGAGGAAATCGCTGCACGCGAACGC
CTCGAGCTGGAGGCGAACAAGCAGGTGCGCGAACTGGCACCGGTGCGTCTGGAGGTCATCCAGATCAATTACGCCAAGGC
GGCAGATCTGGTGGAGCTGCTCAAGCAGGACAAGGACCTGATCTCCGAACGTGGGTTCATTTCCTCTGATGCGCGGACCA
ATACCATTTCCATCCGCGAAACGGCAGAAAAGATCGCCCAGGTACGCAAGCTGATCCAGACTTGGGACGTGCCCGTGCGC
CAGGTGCTGATCGAGGCCCGTATCGTGCGTGCCCGGACGGATGTCACCTCGGATCTGGGAGTGCAGTGGGGTGGCGGTCA
CAAGGCGGTTCGTGGTCGCAATGTCTACTACGTCGGGGGCAGCAATAACCAGGTCCAACAACAGGCTAACAATAATGGCA
GCCCCAACGCCGGTGATCAGGATTTTGTGAGCAACAACTACGCGTTCCCCGATGCCTACGCGGTTGATCTGGCGGTTACC
AAGGCCAATACCTCGTCACTGGCACTGGGTTTTGCGCGTGATAACCTGCTCCTGAACCTTGAGCTTTCCGCTCTGGAAGC
AGACGGCAAGGGTGAGATTGTCGCTCAGCCCAAGGTGGTGACGGCTGATCGCCAGACTGCACTGATCGAATCCGGTGAGG
AAATTCCGTATCAGGAGGCCAGCTCCAGCGGCGCAACTTCGGTCTCCTTCAAGAAGGCGGTGCTGTCACTGGAAGTCACG
CCCCAGATCACCCCGGATGACAAGATCATCATGGATCTGCGCGTGAACCAGGATTCCCGCGGTGAAGTGACCGCCGGTAT
CCCGAGCATCGATACCAACGAGGTCAAGACTCAGGTGCTGGTGGGCAATGGTGAAACGGTGGTGCTTGGCGGCATCTTCA
CGTCGGAAGAGACCAATACCGTCACCAAGACGCCGTTCCTGGGGGACATCCCCTATCTGGGCCGGCTCTTCACCAAGAAA
GAATCCAACAACCAGCGTTCCGAGTTGCTGATCTTCATTACACCGAAACTCCTCAAGAACGGTCTGGTGGAGCAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(504-658)

AMIN domain (AMIN)

(4-90)

Bacterial type II/III secretion system short domain (Secretin_N)

(338-401)

Secretin and TonB N terminus short domain (STN)

(265-311)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

52.733

100

0.537
  comQ Acinetobacter baylyi ADP1

42.898

100

0.45
  pilQ Acinetobacter baumannii strain A118

41.761

100

0.442
  pilQ Acinetobacter baumannii D1279779

41.761

100

0.442
  pilQ Legionella pneumophila strain Lp02

40.444

100

0.411
  pilQ Legionella pneumophila strain ERS1305867

40.597

100

0.409
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.857

85.263

0.365
  pilQ Vibrio cholerae strain A1552

42.857

85.263

0.365

Multiple sequence alignment