Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAH980_RS13755 Genome accession   NZ_CP154856
Coordinates   2917502..2919214 (-) Length   570 a.a.
NCBI ID   WP_004710829.1    Uniprot ID   K9BAJ6
Organism   Acinetobacter nosocomialis strain Ab25     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2912502..2924214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAH980_RS13725 (AAH980_13725) - 2912666..2912992 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  AAH980_RS13730 (AAH980_13730) rlmB 2913094..2913843 (+) 750 WP_002156969.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AAH980_RS13735 (AAH980_13735) - 2913879..2914793 (+) 915 WP_284080461.1 DMT family transporter -
  AAH980_RS13740 (AAH980_13740) coaE 2914790..2915386 (-) 597 WP_004883551.1 dephospho-CoA kinase -
  AAH980_RS13745 (AAH980_13745) pilD 2915388..2916248 (-) 861 WP_004710834.1 A24 family peptidase Machinery gene
  AAH980_RS13750 (AAH980_13750) pilC 2916248..2917474 (-) 1227 WP_004710832.1 type II secretion system F family protein Machinery gene
  AAH980_RS13755 (AAH980_13755) pilB 2917502..2919214 (-) 1713 WP_004710829.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAH980_RS13760 (AAH980_13760) tpiA 2919505..2920299 (+) 795 WP_004710827.1 triose-phosphate isomerase -
  AAH980_RS13765 (AAH980_13765) secG 2920312..2920641 (+) 330 WP_002156913.1 preprotein translocase subunit SecG -
  AAH980_RS13785 (AAH980_13785) rimP 2921278..2921802 (+) 525 WP_002157009.1 ribosome maturation factor RimP -
  AAH980_RS13790 (AAH980_13790) nusA 2921840..2923324 (+) 1485 WP_002156952.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63457.85 Da        Isoelectric Point: 5.2413

>NTDB_id=994100 AAH980_RS13755 WP_004710829.1 2917502..2919214(-) (pilB) [Acinetobacter nosocomialis strain Ab25]
MSVLHTSPKFTGFFRRLVEEKHVSAATMQNALDAAKRAKQDTVAYLIEEVHLSPSLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVSLDGPTTQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEEQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=994100 AAH980_RS13755 WP_004710829.1 2917502..2919214(-) (pilB) [Acinetobacter nosocomialis strain Ab25]
ATGTCAGTATTACATACATCTCCAAAATTTACGGGGTTTTTTCGGCGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAAATGCATTAGATGCAGCAAAGAGAGCCAAGCAGGATACAGTCGCATATTTAATTGAAGAGGTACATCTCTCCC
CTTCTTTATTGGCTGAAACCATTTCTGCGGAGTTTGCTGAACCATATTTTGATCTAGATGTTTATGACACATCTCAGATT
CCTAAAGATCTGGTCGATCAAAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGACAAATTCTATATGT
CGCCACCAGCAATCCAAGTAATATTGAAGCAATCGATGCAATCCGTTTTAACAGTAAATTGCTGGTTGAACCTGTTATTG
TCGAGCATCATAAGCTGGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTTAATGATGAAGAGTTT
GATCTTGACGTCAGCCTTGATGGTCCTACCACTCAAGAAGATGAAGAAGAAACACCTCAAGGTGATGAAGCTCCTATTGT
TAAATATATTAATAAGTTGCTTATTGATGCCATTCGTATGGGCGCGTCAGATTTACATTTTGAGCCTTATGAAAAGTCAT
ATCGGGTACGTTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCGCCTTTACAACTTGCAAACCGTTTGGCATCA
CGCTTAAAAGTAATGTCCCAAATGGACATTTCCGAAAAACGTGTCCCACAAGATGGTCGTATTAAACTTAAGCTATCAAA
AACCAAAGCAATTGATTTCCGTGTGAACTCTCTCCCTACCTTGTTTGGTGAAAAATTAGTTCTACGTATTCTTGATCCAT
CAAGCGCAATGCTAGGCATTGATGCATTAGGCTACGAAGAAGAACAAAAAGCTTTATTTATGGAAGCTTTAGATAAACCA
CAAGGTATGCTTTTGATTACTGGACCAACAGGTTCCGGTAAAACTGTATCTCTATATACAGGCTTAAATATTTTAAATAC
TGAAAGTTCCAATATTTCTACTGCCGAAGATCCAGTGGAAATTAACTTAGAAGGTATTAATCAGGTTAACGTCAATCCTA
AAGTGGGTTTAACATTTGCAGCTGCTCTTAAGTCTTTTTTACGTCAAGACCCTGACATTATTATGGTCGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCTGCTCAAACCGGCCACATGGTGATGTCAACACTACATACCAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAATCTAGTGATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGTAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACATCCAGATTTTCGGGTTTTTCAACCCGTGGGCTGTCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCGGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTAACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9BAJ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

98.596

100

0.986
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.794

99.474

0.505
  pilF Neisseria gonorrhoeae MS11

50.178

98.596

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

51.698

92.982

0.481
  pilB Vibrio campbellii strain DS40M4

50.867

91.053

0.463
  pilB Vibrio cholerae strain A1552

50.29

90.702

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365

Multiple sequence alignment