Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AASM09_RS05360 Genome accession   NZ_CP154630
Coordinates   1164266..1165360 (+) Length   364 a.a.
NCBI ID   WP_049426723.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain C52     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1159266..1170360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AASM09_RS05330 (AASM09_05330) soxR 1159369..1159803 (+) 435 WP_049430131.1 redox-sensitive transcriptional activator SoxR -
  AASM09_RS05335 (AASM09_05335) - 1159854..1160456 (-) 603 WP_049430130.1 short chain dehydrogenase -
  AASM09_RS05340 (AASM09_05340) - 1160553..1161428 (+) 876 WP_100443775.1 LysR family transcriptional regulator -
  AASM09_RS05345 (AASM09_05345) proC 1161477..1162298 (-) 822 WP_049430129.1 pyrroline-5-carboxylate reductase -
  AASM09_RS05350 (AASM09_05350) - 1162317..1162994 (-) 678 WP_049430128.1 YggS family pyridoxal phosphate-dependent enzyme -
  AASM09_RS05355 (AASM09_05355) pilT 1163082..1164119 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  AASM09_RS05360 (AASM09_05360) pilU 1164266..1165360 (+) 1095 WP_049426723.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AASM09_RS05365 (AASM09_05365) - 1165565..1166548 (+) 984 WP_049426721.1 aldo/keto reductase -
  AASM09_RS05370 (AASM09_05370) - 1166620..1167519 (-) 900 WP_049426722.1 LysR family transcriptional regulator -
  AASM09_RS05375 (AASM09_05375) - 1167982..1168194 (+) 213 WP_014646220.1 DUF4287 domain-containing protein -
  AASM09_RS05380 (AASM09_05380) - 1168305..1169012 (+) 708 WP_049427633.1 YitT family protein -
  AASM09_RS05385 (AASM09_05385) - 1169096..1169641 (-) 546 WP_049427629.1 DNA-3-methyladenine glycosylase I -
  AASM09_RS05390 (AASM09_05390) - 1169730..1170296 (+) 567 WP_049427628.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40555.59 Da        Isoelectric Point: 6.7680

>NTDB_id=993824 AASM09_RS05360 WP_049426723.1 1164266..1165360(+) (pilU) [Stenotrophomonas maltophilia strain C52]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=993824 AASM09_RS05360 WP_049426723.1 1164266..1165360(+) (pilU) [Stenotrophomonas maltophilia strain C52]
ATGGCGCACCAGCGCGCCTCTGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTGACGCCCCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCACAGCGCGAGGAAT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTTGGCCGCTTCCGTGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAGGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGTGCCACCGGTACCGGTAAATCGACTTCGCTTGCGGCGA
TGATCGGCTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAA
GGCTGCATCATCACCCAGCGTGAGGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATCGGTGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAGACCGGTCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGTTC
GCGCAAGGTCGCCATGGAGATCCTGCTCGGCACGCCGCTGGTGCAGGATTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAG
ATCAGCTACGAGGACGCGCTGCGCTATGCTGACTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGCACGTTGTCACAGGGCCTGGATGGCGTTGAGATCTCCGAGGTCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363

Multiple sequence alignment