Detailed information NTG0993676

Bioinformatically predicted

Overview

• Name: comEA/celA/cilE
• Type: Machinery gene
• Locus tag: AAIS09_RS04390
• Genome accession: NZ_CP154595
• Coordinates: 856571..857221 (+)
• Length: 216 a.a.
• NCBI ID: WP_057546897.1
• Uniprot ID: A0A4J1WRN7
• Organism: Streptococcus pneumoniae strain serotype 4
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 851571..862221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAIS09_RS04360 (AAIS09_04345)	-	852054..852269 (+)	216	WP_001232081.1	YozE family protein	-
AAIS09_RS04365 (AAIS09_04350)	-	852355..853323 (+)	969	WP_000658191.1	PhoH family protein	-
AAIS09_RS04370 (AAIS09_04355)	-	853516..854016 (+)	501	WP_001866231.1	GNAT family N-acetyltransferase	-
AAIS09_RS04375 (AAIS09_04360)	-	854019..854345 (+)	327	Protein_860	TfoX/Sxy family protein	-
AAIS09_RS04380 (AAIS09_04365)	ald	854646..855757 (-)	1112	Protein_861	alanine dehydrogenase	-
AAIS09_RS04385 (AAIS09_04370)	-	855934..856503 (+)	570	WP_061813581.1	GNAT family N-acetyltransferase	-
AAIS09_RS04390 (AAIS09_04375)	comEA/celA/cilE	856571..857221 (+)	651	WP_057546897.1	ComEA family DNA-binding protein	Machinery gene
AAIS09_RS04395 (AAIS09_04380)	comEC/celB	857205..859445 (+)	2241	WP_061813580.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AAIS09_RS04400 (AAIS09_04385)	-	859624..859812 (+)	189	WP_061813579.1	hypothetical protein	-
AAIS09_RS04405 (AAIS09_04390)	-	859845..860432 (+)	588	WP_000939895.1	ATP-binding cassette domain-containing protein	-
AAIS09_RS04410 (AAIS09_04395)	-	860436..861620 (+)	1185	WP_061813578.1	hypothetical protein	-

Sequence

Protein

• Length: 216 a.a.
• Molecular weight: 23238.66 Da
• Isoelectric Point: 5.9204

>NTDB_id=993676 AAIS09_RS04390 WP_057546897.1 856571..857221(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain serotype 4]
MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQAEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKG
AVKSPGIYDLPVGSRVNDAVHKAGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD

Nucleotide

• Length: 651 bp

>NTDB_id=993676 AAIS09_RS04390 WP_057546897.1 856571..857221(+) (comEA/celA/cilE) [Streptococcus pneumoniae strain serotype 4]
ATGGAAGCAATTATCGAGAAAATCAAAGAGTATAAAATCATCGTCATCTGTACTGGTCTGGGCTTGCTTGTAGGAGGATT
TTTCCTGCTAAAACCAGCTCCACAAACACCTGTCAAAGAGACGAATTTGCAGGCTGAAGTCGCAGCTGTTTCCAAGGATT
TGGTATCCGAAAAGGAAGTGAACAAGGAAGAAAAGGAAGAACCCCTTGAACAAGATCTAATCACAGTAGATGTTAAAGGT
GCTGTCAAATCGCCAGGGATTTATGACTTGCCTGTAGGTAGTCGAGTCAATGATGCTGTTCATAAGGCTGGTGGCTTGAC
AGAGCAAGCAGACAGCAAGTCGCTCAATCTAGCTCAGAAAGTTAGTGATGAGGCTCTGGTTTACGTTCCTACTAAGGGAG
AAGAAGCAGTTAGCCAACAGACTGGTTTGGGGACAGCTTCTTCAATAAGCAAGGAAAAGAAGGTCAATCTCAACAAGGCC
AGTCTGGAAGAACTCAAGCAGGTCAAGGGACTGGGAGGAAAACGAGCTCAGGACATTATCGACCATCGTGAGGCAAATGG
CAAGTTCAAGTCAGTAGACGAGCTCAAGAAGGTCTCTGGCATTGGTGGCAAAACAATAGAAAAGCTTAAAGACTATGTTA
CAGTGGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (76-126)

• Helix-hairpin-helix motif (HHH_3) (151-214)

3D structure

• AlphaFold DB: A0A4J1WRN7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA/celA/cilE	Streptococcus pneumoniae Rx1	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae D39	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae R6	99.537	100	0.995
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	96.759	100	0.968
comEA/celA/cilE	Streptococcus mitis NCTC 12261	95.833	100	0.958
comEA/celA/cilE	Streptococcus mitis SK321	89.815	100	0.898
comEA	Lactococcus lactis subsp. cremoris KW2	43.172	100	0.454
comEA	Bacillus subtilis subsp. subtilis str. 168	41.579	87.963	0.366
comEA	Latilactobacillus sakei subsp. sakei 23K	33.476	100	0.361