Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAGQ88_RS15135 Genome accession   NZ_CP152388
Coordinates   3315187..3316353 (-) Length   388 a.a.
NCBI ID   WP_342652809.1    Uniprot ID   -
Organism   Pseudomonas sp. F3-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3310187..3321353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAGQ88_RS15120 (AAGQ88_15120) - 3311219..3312628 (+) 1410 WP_342652806.1 sigma 54-interacting transcriptional regulator -
  AAGQ88_RS15125 (AAGQ88_15125) - 3312905..3314296 (+) 1392 WP_342652807.1 GntP family permease -
  AAGQ88_RS15130 (AAGQ88_15130) - 3314307..3315077 (+) 771 WP_342652808.1 3-hydroxybutyrate dehydrogenase -
  AAGQ88_RS15135 (AAGQ88_15135) pilU 3315187..3316353 (-) 1167 WP_342652809.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAGQ88_RS15140 (AAGQ88_15140) - 3316540..3316815 (+) 276 WP_342652810.1 peptidylprolyl isomerase -
  AAGQ88_RS15145 (AAGQ88_15145) - 3316834..3317775 (-) 942 WP_285426135.1 sugar kinase -
  AAGQ88_RS15150 (AAGQ88_15150) - 3317788..3318993 (-) 1206 WP_342652811.1 amino acid deaminase -
  AAGQ88_RS15155 (AAGQ88_15155) - 3319051..3319815 (-) 765 WP_285426133.1 IclR family transcriptional regulator -
  AAGQ88_RS15160 (AAGQ88_15160) - 3319956..3320345 (-) 390 WP_062381403.1 RidA family protein -
  AAGQ88_RS15165 (AAGQ88_15165) - 3320368..3321159 (-) 792 WP_285426132.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 42816.90 Da        Isoelectric Point: 6.4537

>NTDB_id=988833 AAGQ88_RS15135 WP_342652809.1 3315187..3316353(-) (pilU) [Pseudomonas sp. F3-2]
MDFPALLKVLATQDGSDLYLSTGAPPCAKFNGVLKPLGTEAFKPGDVALIADGIMDAEQRLDFERELEMNLAFSLSGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLHLPPVLLDVVMEKHGLVLFVGATGSGKSTSLAALIDHRNRHASGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTKDGKRRAAVEVMLGTPTIRDLIHRNELTELKGIMEKSTNMGMQTFDNAL
YDLAIEGAISEDEALKNADSANNVRLRLKLHSEGGHSTITTPPPAPQPAAQVDVKDWGLVDERDEPGA

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=988833 AAGQ88_RS15135 WP_342652809.1 3315187..3316353(-) (pilU) [Pseudomonas sp. F3-2]
ATGGATTTCCCGGCGTTGTTGAAGGTTCTTGCCACCCAGGACGGTTCGGATCTCTATTTATCCACGGGCGCGCCACCCTG
CGCCAAGTTCAACGGCGTGCTGAAGCCGCTGGGCACCGAGGCATTCAAGCCTGGCGACGTGGCGCTCATCGCCGACGGCA
TCATGGACGCCGAGCAACGCCTGGACTTCGAGCGTGAACTTGAAATGAACCTGGCGTTTTCCCTTTCGGGTGTCGGGCGT
TTTCGCATCAACATCTTCAAACAGCGCAACGAAGTGTCCATCGTGGCGCGCAACATCAAGCTCGATATTCCCAAATTCGA
AGACCTGCACCTGCCGCCGGTGCTGCTCGATGTGGTGATGGAGAAGCACGGCCTGGTGCTGTTCGTCGGCGCGACGGGCT
CGGGCAAGTCGACGTCGCTGGCTGCCCTGATCGATCACCGCAATCGCCACGCCAGCGGGCACATCATCACCATCGAGGAC
CCGGTCGAATTCATCCATCGGCACAAGAAATCGATCATCAATCAGCGTGAAGTCGGGGTCGACACGCGCAGTTTTCATGC
AGCGCTGAAAAACACCCTGCGTCAGGCGCCGGATGTGATTCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCATG
CGCTGGCTTTCGCCGATACGGGACATCTGGCGATCTCCACGTTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTTCCGGAGGAGCGTCGCGCGCAGCTGTTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCGCAGCG
GCTGGTCAAGACCAAGGATGGCAAGCGCCGAGCCGCCGTTGAAGTGATGCTCGGCACGCCAACCATTCGTGACCTGATCC
ATCGCAACGAGCTCACCGAGCTCAAGGGCATCATGGAAAAGTCCACCAATATGGGCATGCAGACCTTCGACAACGCGTTG
TACGACCTGGCCATCGAAGGCGCGATCAGCGAGGACGAGGCGCTGAAAAACGCCGATTCCGCGAACAACGTGCGGCTGCG
TCTGAAGCTGCACAGCGAAGGCGGTCATTCGACGATCACCACGCCACCTCCCGCGCCACAGCCTGCGGCGCAGGTCGACG
TCAAGGACTGGGGCCTGGTGGATGAGCGCGACGAGCCGGGGGCTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.233

93.041

0.523
  pilU Acinetobacter baylyi ADP1

52.381

92.01

0.482
  pilU Vibrio cholerae strain A1552

52

90.206

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.65

95.103

0.387
  pilT Pseudomonas stutzeri DSM 10701

42.733

88.66

0.379
  pilT Pseudomonas aeruginosa PAK

42.151

88.66

0.374
  pilT Vibrio cholerae strain A1552

42.342

85.825

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.342

85.825

0.363

Multiple sequence alignment