Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAG689_RS17015 Genome accession   NZ_CP152342
Coordinates   3458419..3460155 (+) Length   578 a.a.
NCBI ID   WP_406622994.1    Uniprot ID   -
Organism   Acidovorax sp. SDU_ACID1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3453419..3465155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG689_RS16975 proB 3453989..3455128 (-) 1140 WP_406622990.1 glutamate 5-kinase -
  AAG689_RS16980 - 3455205..3455546 (-) 342 WP_406622991.1 hypothetical protein -
  AAG689_RS16985 - 3455516..3455896 (-) 381 WP_406622992.1 GTPase -
  AAG689_RS16990 - 3455864..3456300 (-) 437 Protein_3342 hypothetical protein -
  AAG689_RS16995 rpmA 3456374..3456631 (-) 258 WP_011804286.1 50S ribosomal protein L27 -
  AAG689_RS17000 rplU 3456647..3456958 (-) 312 WP_024001616.1 50S ribosomal protein L21 -
  AAG689_RS17005 - 3457127..3458110 (+) 984 WP_406622993.1 polyprenyl synthetase family protein -
  AAG689_RS17015 pilB 3458419..3460155 (+) 1737 WP_406622994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAG689_RS17020 pilC 3460175..3461392 (+) 1218 WP_406622995.1 type II secretion system F family protein Machinery gene
  AAG689_RS17025 pilD 3461396..3462274 (+) 879 WP_406622996.1 prepilin peptidase Machinery gene
  AAG689_RS17030 coaE 3462277..3462888 (+) 612 WP_406622997.1 dephospho-CoA kinase -
  AAG689_RS17035 zapD 3462930..3463685 (+) 756 WP_406622998.1 cell division protein ZapD -
  AAG689_RS17040 - 3463691..3463912 (+) 222 WP_406622999.1 DNA gyrase inhibitor YacG -
  AAG689_RS17045 - 3463976..3464401 (-) 426 WP_020229073.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 63173.48 Da        Isoelectric Point: 5.8659

>NTDB_id=988491 AAG689_RS17015 WP_406622994.1 3458419..3460155(+) (pilB) [Acidovorax sp. SDU_ACID1]
MAAVGTPQKESPSIALPGLARALMSVGKLSQKSAEDIYKKSQTSRTNFIAELTGSNTITPSDLAHTVSAIFGAPLVDISA
IDPLRIPKDLLDAKLCQAYKVVALNKRNNRLIVATADPTDQEAAEKIKFTTQMGVDWIIAEHDKLIKLIDATSKSVSETL
DTITSGGDFEFDDIPVEEASESNEAATTEVEDAPIVKFLHKMLLDAFNMRASDLHFEPYEHHYRVRFRIDGELREIASPP
IAIKEKLASRIKVISRLDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPEEKE
RLLHAIGRPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPNCKAPADIP
HETLLEAGYREEEIDGSWVTYKPVGCSACNNGYKGRVGIYQVMPVSEETQRIILRDGSALEIAEQARLEGVRSLRESGLH
KAKIGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=988491 AAG689_RS17015 WP_406622994.1 3458419..3460155(+) (pilB) [Acidovorax sp. SDU_ACID1]
ATGGCTGCCGTAGGTACCCCTCAGAAAGAGTCTCCCTCTATTGCTCTGCCAGGCTTGGCGCGGGCGCTCATGTCTGTGGG
AAAACTGAGCCAGAAATCCGCCGAGGATATTTACAAAAAATCACAAACGAGCCGCACCAATTTCATCGCTGAATTGACGG
GATCCAACACGATCACACCCAGCGATTTGGCACATACGGTGTCTGCGATTTTCGGCGCGCCCTTGGTGGATATCAGCGCT
ATTGATCCGCTGCGCATTCCCAAGGATTTGCTGGACGCCAAGCTGTGCCAGGCTTACAAGGTGGTCGCGCTGAATAAGCG
CAATAACCGCCTGATCGTCGCAACGGCCGATCCCACGGACCAGGAGGCTGCGGAAAAGATCAAATTCACGACCCAGATGG
GCGTGGACTGGATCATCGCCGAGCACGACAAGCTCATCAAACTCATCGATGCGACCTCCAAGAGCGTCAGCGAAACCCTG
GACACGATTACCAGTGGCGGGGATTTTGAATTCGATGACATTCCCGTCGAAGAGGCATCGGAAAGCAACGAGGCAGCGAC
CACCGAGGTCGAAGATGCCCCCATCGTCAAGTTTCTGCACAAGATGCTGCTGGACGCGTTCAACATGCGGGCATCCGATC
TGCATTTCGAACCTTACGAGCACCATTACCGCGTGCGCTTTCGCATCGACGGGGAATTGCGGGAAATCGCCTCGCCTCCC
ATTGCGATCAAGGAAAAGCTGGCTTCCCGGATCAAGGTCATCTCCCGCCTCGATATTTCCGAGAAACGAGTGCCACAAGA
CGGCCGGATGAAGCTCAAGGTGGGCCCCGACCGGGTGATCGACTTCCGTGTAAGCACCCTGCCGACCTTGTTCGGCGAAA
AGATCGTGATCCGTATCCTCGATCCGAGCAGCGCCAAGCTGGGGATCGACGCGCTGGGGTACGAGCCCGAAGAAAAAGAA
CGGCTCCTGCATGCCATCGGCCGGCCCTACGGCATGATTCTGGTGACCGGACCCACGGGTTCCGGCAAGACGGTGTCCCT
CTACACCTGCCTGAACCTGCTGAACAAGCCCGGCGTCAACATCGCGACGGCGGAAGACCCGTCGGAAATCAACCTGCCGG
GCGTGAACCAGGTCAACGTCAACGAGAAGGCGGGGCTTACCTTCGCCACGGCGCTCAAGGCCTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGGGACCTGGAAACCGCGGACATCTCGATCAAGGCCGCACAGACAGGCCATCTGGT
GCTCTCGACCCTGCACACCAACGACGCACCGACGACGTTGACGCGCATGCGCAACATGGGGATCGCCCCCTTCAACATCG
CATCCAGCGTCATCCTGATCACCGCGCAACGCCTCGCGCGCCGCCTGTGCCCCAACTGCAAGGCCCCGGCCGACATTCCG
CATGAAACCCTGCTGGAGGCCGGCTACCGCGAGGAAGAAATCGATGGCTCCTGGGTGACCTACAAGCCCGTCGGATGCTC
CGCCTGCAACAACGGCTACAAGGGACGCGTAGGCATCTACCAGGTCATGCCCGTTTCCGAAGAGACGCAACGCATCATCC
TGCGCGACGGCAGTGCCCTGGAAATCGCGGAGCAGGCCCGGCTGGAAGGGGTGCGCTCCCTGCGCGAGTCCGGCCTGCAC
AAAGCGAAGATCGGCCTGACCTCGCTCGAAGAGGTTCTGGCCGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(58-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.413

100

0.542
  pilB Acinetobacter baumannii D1279779

53.901

97.578

0.526
  pilF Neisseria gonorrhoeae MS11

51.858

97.751

0.507
  pilB Legionella pneumophila strain ERS1305867

49.468

97.578

0.483
  pilB Vibrio cholerae strain A1552

45.161

96.54

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

44.803

96.54

0.433
  pilB Vibrio campbellii strain DS40M4

44.014

98.27

0.433
  pilF Thermus thermophilus HB27

40

97.751

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.054

98.789

0.386

Multiple sequence alignment