Detailed information NTG0988164

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: AAEU35_RS01265
• Genome accession: NZ_CP152294
• Coordinates: 266541..267389 (-)
• Length: 282 a.a.
• NCBI ID: WP_012897825.1
• Uniprot ID: A0A2A9I6Y4
• Organism: Lactococcus lactis strain Q13
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 261541..272389

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAEU35_RS01250	xerS	261617..262687 (+)	1071	WP_003131037.1	tyrosine recombinase XerS	-
AAEU35_RS01255	trmFO	262732..264075 (-)	1344	WP_025017071.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
AAEU35_RS01260	topA	264147..266279 (-)	2133	WP_023189256.1	type I DNA topoisomerase	-
AAEU35_RS01265	dprA	266541..267389 (-)	849	WP_012897825.1	DNA-processing protein DprA	Machinery gene
AAEU35_RS01270	-	267535..267915 (-)	381	WP_003131142.1	RidA family protein	-
AAEU35_RS01275	budA	267956..268666 (-)	711	WP_003131140.1	acetolactate decarboxylase	-
AAEU35_RS01280	ilvA	268782..270032 (-)	1251	WP_017864495.1	threonine ammonia-lyase IlvA	-
AAEU35_RS01285	ilvC	270088..271110 (-)	1023	WP_015426406.1	ketol-acid reductoisomerase	-
AAEU35_RS01290	ilvN	271156..271632 (-)	477	WP_010905839.1	acetolactate synthase small subunit	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 32119.17 Da
• Isoelectric Point: 9.5086

>NTDB_id=988164 AAEU35_RS01265 WP_012897825.1 266541..267389(-) (dprA) [Lactococcus lactis strain Q13]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDVSKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide

• Length: 849 bp

>NTDB_id=988164 AAEU35_RS01265 WP_012897825.1 266541..267389(-) (dprA) [Lactococcus lactis strain Q13]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGACGTAAGTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGACTCAAGGAAATTTACAATCCACCGGTTCTATTATTTTATCAAGGAGATATAAGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAGTATCTTGCTAAAAACCA
ACTCATACTATCAGAATATTCAGTCGGAGAAAAGCCACTTCGTTATCATTTTCCTGAACGTAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATACAACAAGGAGCAAA
ATTAATCTATCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

• DNA recombination-mediator protein A (DNA_processg_A) (76-270)

3D structure

• AlphaFold DB: A0A2A9I6Y4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	86.879	100	0.869
dprA	Streptococcus mutans UA159	60.072	98.582	0.592
dprA/cilB/dalA	Streptococcus mitis SK321	54.643	99.291	0.543
dprA/cilB/dalA	Streptococcus pneumoniae R6	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae D39	53.929	99.291	0.535
dprA	Legionella pneumophila strain ERS1305867	39.13	97.872	0.383
dprA	Haemophilus influenzae Rd KW20	38.889	95.745	0.372
dprA	Bacillus subtilis subsp. subtilis str. 168	43.777	82.624	0.362
dprA	Latilactobacillus sakei subsp. sakei 23K	40	90.426	0.362