Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R8624_RS09450 Genome accession   NZ_AP026930
Coordinates   1826179..1827195 (-) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900701208     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1821179..1832195
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8624_RS09405 - 1821257..1821388 (-) 132 WP_000768904.1 hypothetical protein -
  R8624_RS09410 (PC1195_18280) - 1821389..1822579 (-) 1191 WP_000167766.1 acetate kinase -
  R8624_RS09415 (PC1195_18290) comYH 1822630..1823583 (-) 954 WP_050311715.1 class I SAM-dependent methyltransferase Machinery gene
  R8624_RS09420 - 1823644..1824238 (-) 595 Protein_1832 class I SAM-dependent methyltransferase -
  R8624_RS09425 (PC1195_18320) comGG/cglG 1824341..1824787 (-) 447 WP_050311716.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8624_RS09430 (PC1195_18330) comGF/cglF 1824765..1825226 (-) 462 WP_050120523.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8624_RS09435 (PC1195_18340) comGE/cglE 1825189..1825491 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8624_RS09440 (PC1195_18350) comGD/cglD 1825454..1825858 (-) 405 WP_050311717.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8624_RS09445 (PC1195_18360) comGC/cglC 1825851..1826177 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  R8624_RS09450 (PC1195_18370) comGB/cglB 1826179..1827195 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8624_RS09455 (PC1195_18380) comGA/cglA/cilD 1827143..1828084 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  R8624_RS09460 (PC1195_18390) - 1828160..1828525 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8624_RS09465 (PC1195_18400) - 1828676..1829734 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R8624_RS09470 (PC1195_18410) nagA 1829897..1831048 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=98778 R8624_RS09450 WP_074017570.1 1826179..1827195(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900701208]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=98778 R8624_RS09450 WP_074017570.1 1826179..1827195(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900701208]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGTTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTGATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment