Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACKVDN_RS23550 Genome accession   NZ_CP183129
Coordinates   4835097..4836482 (+) Length   461 a.a.
NCBI ID   WP_001025214.1    Uniprot ID   -
Organism   Escherichia coli strain 755     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4830097..4841482
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKVDN_RS23525 (ACKVDN_23495) aroP 4830645..4832018 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  ACKVDN_RS23530 (ACKVDN_23500) ampE 4832061..4832915 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  ACKVDN_RS23535 (ACKVDN_23505) ampD 4832912..4833463 (-) 552 WP_000923734.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKVDN_RS23540 (ACKVDN_23510) nadC 4833551..4834444 (+) 894 WP_001135164.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKVDN_RS23545 (ACKVDN_23515) pilA 4834647..4835087 (+) 441 WP_000360907.1 prepilin peptidase-dependent pilin Machinery gene
  ACKVDN_RS23550 (ACKVDN_23520) pilB 4835097..4836482 (+) 1386 WP_001025214.1 type II secretion system protein GspE Machinery gene
  ACKVDN_RS23555 (ACKVDN_23525) hofC 4836472..4837674 (+) 1203 WP_000157225.1 protein transport protein HofC -
  ACKVDN_RS23560 (ACKVDN_23530) guaC 4837709..4838752 (-) 1044 WP_001217337.1 GMP reductase -
  ACKVDN_RS23565 (ACKVDN_23535) - 4838908..4838952 (-) 45 WP_120795372.1 protein YacM -
  ACKVDN_RS23570 (ACKVDN_23540) coaE 4838977..4839597 (+) 621 WP_001269523.1 dephospho-CoA kinase -
  ACKVDN_RS23575 (ACKVDN_23545) zapD 4839597..4840340 (+) 744 WP_001194731.1 cell division protein ZapD -
  ACKVDN_RS23580 (ACKVDN_23550) yacG 4840350..4840547 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  ACKVDN_RS23585 (ACKVDN_23555) mutT 4840749..4841147 (-) 399 WP_000736024.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50639.36 Da        Isoelectric Point: 6.5396

>NTDB_id=982376 ACKVDN_RS23550 WP_001025214.1 4835097..4836482(+) (pilB) [Escherichia coli strain 755]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSNELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAIHEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLEMHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=982376 ACKVDN_RS23550 WP_001025214.1 4835097..4836482(+) (pilB) [Escherichia coli strain 755]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTGGTCGATGCCCCTTCGAATGAACTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTATTCATGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAATCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATACGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGATGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGGAACCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAGTCAGGCACTGGATGTCAACACGCTTGGAATGCAGCCGTTACAACTGGCGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACAGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATAAAAGCCGCACAAACCGGTCACCTGGTGTTGTCCACGCTACACACCAATTCC
ACCTGCGAAACGCTGGTGCGTTTACAGCAAATGGGCGTTGCTCGCTGGATGCTCTCATCGGCACTTACGCTGGTAATAGC
CCAGCGTCTGGTACGTAAACTTTGCCCACATTGTCGCCGACAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAATGCACGCCCGACA
GGCGGGTATGCGTACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

41.469

100

0.416
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Haemophilus influenzae 86-028NP

40.127

100

0.41
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.41

84.599

0.393
  pilB Acinetobacter baylyi ADP1

45.478

83.948

0.382
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Acinetobacter baumannii D1279779

44.186

83.948

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371
  pilF Thermus thermophilus HB27

40.587

88.72

0.36