Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACKVDN_RS23545 Genome accession   NZ_CP183129
Coordinates   4834647..4835087 (+) Length   146 a.a.
NCBI ID   WP_000360907.1    Uniprot ID   -
Organism   Escherichia coli strain 755     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4829647..4840087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACKVDN_RS23525 (ACKVDN_23495) aroP 4830645..4832018 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  ACKVDN_RS23530 (ACKVDN_23500) ampE 4832061..4832915 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  ACKVDN_RS23535 (ACKVDN_23505) ampD 4832912..4833463 (-) 552 WP_000923734.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACKVDN_RS23540 (ACKVDN_23510) nadC 4833551..4834444 (+) 894 WP_001135164.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACKVDN_RS23545 (ACKVDN_23515) pilA 4834647..4835087 (+) 441 WP_000360907.1 prepilin peptidase-dependent pilin Machinery gene
  ACKVDN_RS23550 (ACKVDN_23520) pilB 4835097..4836482 (+) 1386 WP_001025214.1 type II secretion system protein GspE Machinery gene
  ACKVDN_RS23555 (ACKVDN_23525) hofC 4836472..4837674 (+) 1203 WP_000157225.1 protein transport protein HofC -
  ACKVDN_RS23560 (ACKVDN_23530) guaC 4837709..4838752 (-) 1044 WP_001217337.1 GMP reductase -
  ACKVDN_RS23565 (ACKVDN_23535) - 4838908..4838952 (-) 45 WP_120795372.1 protein YacM -
  ACKVDN_RS23570 (ACKVDN_23540) coaE 4838977..4839597 (+) 621 WP_001269523.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15651.78 Da        Isoelectric Point: 4.3938

>NTDB_id=982375 ACKVDN_RS23545 WP_000360907.1 4834647..4835087(+) (pilA) [Escherichia coli strain 755]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSTMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=982375 ACKVDN_RS23545 WP_000360907.1 4834647..4835087(+) (pilA) [Escherichia coli strain 755]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGTGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGTAATGGCATCCCCTCGCCTACCACTACCCGC
TATGTTTCAACCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGTTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment