Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   ACNR0F_RS10595 Genome accession   NZ_CP183037
Coordinates   1950715..1951143 (+) Length   142 a.a.
NCBI ID   WP_019390096.1    Uniprot ID   B6SE74
Organism   Kingella kingae strain URMC_2412A945     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1945715..1956143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNR0F_RS10575 (ACNR0F_10575) - 1946487..1947032 (-) 546 WP_038310304.1 gamma carbonic anhydrase family protein -
  ACNR0F_RS10580 (ACNR0F_10580) - 1947072..1947806 (-) 735 WP_003791735.1 histidine phosphatase family protein -
  ACNR0F_RS10585 (ACNR0F_10585) recJ 1948023..1949735 (-) 1713 WP_038310302.1 single-stranded-DNA-specific exonuclease RecJ -
  ACNR0F_RS10590 (ACNR0F_10590) pilE 1950095..1950568 (+) 474 WP_418604913.1 pilin Machinery gene
  ACNR0F_RS10595 (ACNR0F_10595) pilE 1950715..1951143 (+) 429 WP_019390096.1 pilin Machinery gene
  ACNR0F_RS10600 (ACNR0F_10600) - 1951155..1951331 (+) 177 WP_418604914.1 hypothetical protein -
  ACNR0F_RS10605 (ACNR0F_10605) - 1951261..1951932 (+) 672 WP_418604916.1 fimb protein -
  ACNR0F_RS10615 (ACNR0F_10615) - 1952378..1953267 (-) 890 Protein_2053 metal ABC transporter permease -
  ACNR0F_RS10620 (ACNR0F_10620) - 1953491..1953685 (-) 195 Protein_2054 pyrimidine dimer DNA glycosylase/endonuclease V -
  ACNR0F_RS10625 (ACNR0F_10625) - 1953734..1954459 (-) 726 WP_038302563.1 metal ABC transporter ATP-binding protein -
  ACNR0F_RS10630 (ACNR0F_10630) - 1954462..1954620 (-) 159 WP_418604917.1 hypothetical protein -
  ACNR0F_RS10635 (ACNR0F_10635) - 1954775..1955620 (-) 846 WP_003791720.1 mechanosensitive ion channel family protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14865.01 Da        Isoelectric Point: 7.8822

>NTDB_id=981493 ACNR0F_RS10595 WP_019390096.1 1950715..1951143(+) (pilE) [Kingella kingae strain URMC_2412A945]
METKMQGFTLIELMIVIAIVGILAALALPTYQDFSVRSRVAEGLSLAGGAKTSVAEYYASHNQFPANNVTAGLATANQIK
GSSVHSLTVTDGVITVVFNNKVAAAGKNSLVLTPSVAKEGSITWDCHGGTLDGRLRPSECRN

Nucleotide


Download         Length: 429 bp        

>NTDB_id=981493 ACNR0F_RS10595 WP_019390096.1 1950715..1951143(+) (pilE) [Kingella kingae strain URMC_2412A945]
ATGGAAACTAAAATGCAAGGTTTTACCTTAATTGAATTGATGATTGTTATTGCTATTGTGGGTATTTTAGCTGCGCTGGC
ATTGCCAACGTATCAGGATTTTTCAGTACGCTCGCGTGTGGCAGAAGGATTGAGTTTGGCTGGTGGTGCAAAAACATCGG
TGGCAGAATACTATGCTTCGCATAACCAATTTCCAGCCAATAATGTAACGGCAGGTTTGGCTACAGCCAACCAAATTAAG
GGCAGTTCGGTGCATTCGCTTACGGTAACAGATGGTGTGATTACGGTGGTATTTAATAATAAAGTGGCGGCAGCAGGTAA
AAATTCGCTGGTTTTAACGCCTAGTGTGGCGAAAGAAGGCTCTATTACTTGGGATTGCCACGGTGGTACATTGGACGGCA
GATTGCGTCCATCGGAATGCCGTAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)

Pilin (bacterial filament) (Pilin)

(36-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B6SE74

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

40.244

100

0.465
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.465
  pilE Neisseria gonorrhoeae strain FA1090

39.877

100

0.458
  pilA/pilA1 Eikenella corrodens VA1

41.935

100

0.458
  pilA2 Legionella pneumophila str. Paris

45.324

97.887

0.444
  comP Acinetobacter baylyi ADP1

40.26

100

0.437
  pilA2 Legionella pneumophila strain ERS1305867

44.604

97.887

0.437
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.806

100

0.373