Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAFX34_RS10975 Genome accession   NZ_CP151937
Coordinates   718529..720214 (-) Length   561 a.a.
NCBI ID   WP_061058908.1    Uniprot ID   -
Organism   Vibrio vulnificus strain TBV029     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 713529..725214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAFX34_RS10945 (AAFX34_10935) rplS 714043..714396 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  AAFX34_RS10950 (AAFX34_10940) yacG 714653..714847 (-) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  AAFX34_RS10955 (AAFX34_10945) zapD 714910..715650 (-) 741 WP_011079546.1 cell division protein ZapD -
  AAFX34_RS10960 (AAFX34_10950) coaE 715683..716291 (-) 609 WP_061058911.1 dephospho-CoA kinase -
  AAFX34_RS10965 (AAFX34_10955) pilD 716293..717162 (-) 870 WP_061058910.1 prepilin peptidase Machinery gene
  AAFX34_RS10970 (AAFX34_10960) pilC 717256..718482 (-) 1227 WP_061058909.1 type II secretion system F family protein Machinery gene
  AAFX34_RS10975 (AAFX34_10965) pilB 718529..720214 (-) 1686 WP_061058908.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAFX34_RS10980 (AAFX34_10970) - 720219..720668 (-) 450 WP_175546417.1 pilin -
  AAFX34_RS10985 (AAFX34_10975) nadC 720891..721778 (-) 888 WP_061058907.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAFX34_RS10990 (AAFX34_10980) ampD 721871..722443 (+) 573 WP_045622095.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AAFX34_RS10995 (AAFX34_10985) pdhR 722832..723599 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62279.28 Da        Isoelectric Point: 5.5296

>NTDB_id=981322 AAFX34_RS10975 WP_061058908.1 718529..720214(-) (pilB) [Vibrio vulnificus strain TBV029]
MTNLATILRQANQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPCTDLNQYDYQKLCNQLGLREL
ITRHQALPLQKSNSGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELAN
LVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGMI
LMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADDL
IYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLAQQKGMMTLKDSGLEKLKQGITSLEELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=981322 AAFX34_RS10975 WP_061058908.1 718529..720214(-) (pilB) [Vibrio vulnificus strain TBV029]
ATGACTAACCTTGCCACAATTCTTCGTCAGGCCAATCAACTTAGCCTAACGCAAGAACAAGCGTGTCGAGAGACAATACA
AGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCGGATGAGTTAACCGAGCAGCTAAGCG
CGATTTTTGGCTTACCATGTACTGACCTCAACCAATATGACTACCAAAAGCTGTGTAATCAACTTGGGTTACGTGAGCTG
ATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACTCCGGTTTAATACTCGCGGTTGCAGACCCTACCAATATTCA
AGCTGAAGATGATTTCCGTTTTGCCACAGGTGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGGCAA
TTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGTTGGCGAAT
TTGGTTGAGGTTTCAGATGACGAAGTCGAGTCAATTGAAGACTTAAGCCAAGATGAATCCCCCGTCAGTCGCTTTATCAA
CCAAATCCTGCTGGATGCGGTGCGCAAAGGCGCATCTGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGCGAT
TGCGTTGCGATGGTATTCTGGTGGAAGTGCAGCAGCCTCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAGATC
CTTTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGCGAT
TGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTAGCT
TGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATGATT
TTGATGACAGGCCCAACAGGAAGCGGTAAAACCGTTTCTCTGTACACAGGCTTGAGTTTGCTTAACCGGCCAGAAGTGAA
TATCTCCACCGCGGAAGACCCGGTCGAAATTAACCTCCCAGGTATCAACCAAGTGCAAGTGAAACCCAAAATTGGTTTTG
GTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCAGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAAACC
GCCGAGATCGCCGTCAAAGCAGCCCAAACCGGCCACCTTGTGCTATCCACCTTGCATACCAACTCGGCTGCTGAAACCAT
TGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGGCTC
GTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCCACATTTGAGTTACTCGGCCTAAAAGCTGACGACTTG
ATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCATGCC
TTTTGATGAGCAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTTGCTCAGCAAAAAGGCA
TGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTCTAT
TTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

77.005

100

0.77
  pilB Vibrio parahaemolyticus RIMD 2210633

76.071

99.822

0.759
  pilB Vibrio cholerae strain A1552

74.555

100

0.747
  pilB Legionella pneumophila strain ERS1305867

53.021

91.444

0.485
  pilB Acinetobacter baumannii D1279779

51.731

92.692

0.48
  pilB Acinetobacter baylyi ADP1

50.478

93.226

0.471
  pilF Neisseria gonorrhoeae MS11

45.293

100

0.455
  pilF Thermus thermophilus HB27

40.608

99.643

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.474

0.364
  pilB Haemophilus influenzae 86-028NP

41.598

86.988

0.362
  pilB Haemophilus influenzae Rd KW20

41.736

86.275

0.36

Multiple sequence alignment