Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAEO81_RS17630 Genome accession   NZ_CP151650
Coordinates   3852996..3854162 (-) Length   388 a.a.
NCBI ID   WP_341958225.1    Uniprot ID   -
Organism   Pseudomonas sp. RC10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3847996..3859162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEO81_RS17615 (AAEO81_17615) - 3848845..3850263 (+) 1419 WP_341958220.1 sigma 54-interacting transcriptional regulator -
  AAEO81_RS17620 (AAEO81_17620) - 3850660..3852051 (+) 1392 WP_341958222.1 GntP family permease -
  AAEO81_RS17625 (AAEO81_17625) - 3852062..3852832 (+) 771 WP_166598664.1 3-hydroxybutyrate dehydrogenase -
  AAEO81_RS17630 (AAEO81_17630) pilU 3852996..3854162 (-) 1167 WP_341958225.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAEO81_RS17635 (AAEO81_17635) - 3854344..3854619 (+) 276 WP_037009819.1 peptidylprolyl isomerase -
  AAEO81_RS17640 (AAEO81_17640) - 3854748..3855689 (-) 942 WP_166598662.1 sugar kinase -
  AAEO81_RS17645 (AAEO81_17645) - 3855702..3856925 (-) 1224 WP_341958232.1 amino acid deaminase -
  AAEO81_RS17650 (AAEO81_17650) - 3856968..3857732 (-) 765 WP_341958234.1 IclR family transcriptional regulator -
  AAEO81_RS17655 (AAEO81_17655) - 3857870..3858262 (-) 393 WP_166598659.1 RidA family protein -
  AAEO81_RS17660 (AAEO81_17660) - 3858287..3859078 (-) 792 WP_341958237.1 amino acid ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 388 a.a.        Molecular weight: 42757.81 Da        Isoelectric Point: 6.3931

>NTDB_id=979515 AAEO81_RS17630 WP_341958225.1 3852996..3854162(-) (pilU) [Pseudomonas sp. RC10]
MDFPALLKVLATQDGSDLYLSTGAPPCAKFNGVLKPLGTEAFKPGDVALIADGIMDAEQRADFERELEMNLAFSLSGVGR
FRINIFKQRNEVSIVARNIKLDIPKFEDLHLPPILLDVVMEKHGLVLFVGATGSGKSTSLAALIDHRNRHASGHIITIED
PVEFIHKHKRSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLHDLGNNLKAFVSQRLVKTTDGKRRAAVEVMLGTSTIRDLIHRNELTELKGIMEKSVNLGMQTFDNAL
FDLAVEGAITEEEALKNADSANNVRLRLKLHAEGDANTISTPPPPPQPAAKANVKDWGLVDERDEPGS

Nucleotide


Download         Length: 1167 bp        

>NTDB_id=979515 AAEO81_RS17630 WP_341958225.1 3852996..3854162(-) (pilU) [Pseudomonas sp. RC10]
ATGGATTTTCCGGCGTTGTTGAAGGTTTTGGCCACCCAGGATGGATCGGATCTCTACCTGTCTACCGGTGCGCCGCCCTG
CGCGAAATTCAATGGCGTCCTCAAGCCATTGGGCACTGAGGCGTTCAAACCCGGCGACGTCGCGCTGATTGCCGACGGGA
TCATGGACGCCGAGCAGCGGGCGGACTTCGAGCGTGAGCTGGAGATGAACCTGGCGTTCTCCTTGTCGGGCGTCGGACGG
TTCCGGATCAACATCTTCAAACAGCGCAACGAAGTGTCCATCGTGGCGCGGAACATCAAGCTCGACATCCCGAAATTCGA
AGACCTGCACCTACCACCGATCCTGCTCGACGTGGTCATGGAAAAGCACGGGCTGGTGCTGTTCGTCGGGGCCACGGGCT
CGGGCAAATCCACCTCGCTGGCCGCGTTGATCGACCACCGAAACCGGCACGCCAGCGGGCACATCATCACCATTGAAGAC
CCGGTGGAGTTCATCCACAAACACAAGCGCTCGATCATCAATCAGCGGGAAGTCGGGGTCGACACCCGCAGCTTTCACGC
TGCATTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATCCTGATCGGGGAAATCCGTGACCGGGAAACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGGCATTTGGCGATTTCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTTTTCCCAGAAGAACGCCGCCCGCAGTTGCTGCATGACCTGGGCAACAACCTGAAGGCGTTCGTTTCCCAGCG
GCTGGTCAAGACCACGGATGGCAAGCGTCGAGCGGCCGTGGAAGTGATGCTCGGCACGTCAACCATTCGCGACCTGATCC
ATCGCAATGAACTGACTGAGCTCAAAGGCATCATGGAAAAATCCGTGAACCTGGGGATGCAGACCTTTGATAACGCGCTG
TTCGACCTGGCGGTGGAGGGGGCGATCACCGAAGAAGAGGCGCTGAAAAATGCTGATTCCGCAAACAACGTGCGTTTGCG
CCTGAAGCTCCACGCCGAGGGCGATGCGAACACGATCTCGACGCCTCCGCCGCCTCCACAGCCCGCCGCGAAAGCAAACG
TCAAGGATTGGGGGCTGGTGGATGAGCGGGATGAGCCAGGGAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.249

93.299

0.515
  pilU Acinetobacter baylyi ADP1

52.661

92.01

0.485
  pilU Vibrio cholerae strain A1552

51.429

90.206

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.701

95.619

0.389
  pilT Pseudomonas stutzeri DSM 10701

41.57

88.66

0.369
  pilT Pseudomonas aeruginosa PAK

41.279

88.66

0.366
  pilT Vibrio cholerae strain A1552

42.042

85.825

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.042

85.825

0.361

Multiple sequence alignment