Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AADV58_RS04585 Genome accession   NZ_CP151406
Coordinates   945393..947108 (-) Length   571 a.a.
NCBI ID   WP_028994944.1    Uniprot ID   -
Organism   Azonexus hydrophilus strain NCP973     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 940393..952108
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADV58_RS04560 (AADV58_04560) aspS 940544..942343 (-) 1800 WP_028994939.1 aspartate--tRNA ligase -
  AADV58_RS04565 (AADV58_04565) - 942363..942977 (-) 615 WP_028994940.1 DUF502 domain-containing protein -
  AADV58_RS04570 (AADV58_04570) - 942977..943228 (-) 252 WP_028994941.1 zinc ribbon domain-containing protein -
  AADV58_RS04575 (AADV58_04575) pilD 943299..944147 (-) 849 WP_281985530.1 A24 family peptidase Machinery gene
  AADV58_RS04580 (AADV58_04580) pilC 944147..945379 (-) 1233 WP_028994943.1 type II secretion system F family protein Machinery gene
  AADV58_RS04585 (AADV58_04585) pilB 945393..947108 (-) 1716 WP_028994944.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AADV58_RS04590 (AADV58_04590) - 947153..948460 (-) 1308 WP_051295367.1 HlyC/CorC family transporter -
  AADV58_RS04595 (AADV58_04595) ccsA 948462..949301 (-) 840 WP_028994945.1 cytochrome c biogenesis protein CcsA -
  AADV58_RS04600 (AADV58_04600) ffh 949331..950710 (+) 1380 WP_028994946.1 signal recognition particle protein -
  AADV58_RS04605 (AADV58_04605) - 950711..951325 (-) 615 WP_028994947.1 transglycosylase SLT domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62209.67 Da        Isoelectric Point: 5.5919

>NTDB_id=976444 AADV58_RS04585 WP_028994944.1 945393..947108(-) (pilB) [Azonexus hydrophilus strain NCP973]
MAATPQNSPLSGLARALVQAGRLKEGEAEELAQQALAAKLPLIEQIIVAKKATSLEIARFSAETFGYPLFDLDAIDERHI
PQDAIDRKLIATHKVVPLNKRGNRLSVAIADPTNLRALDEIRFQTGLTVDPIVVEHPKLLPLVAKFSESASDALKNLADD
DINLDFLDEEAAGKTDEASAQEIDDAPVVKFIQKMLLDAINDGASDIHFEPYEKYYRIRFRVDGILREVATPPLAIKEKI
ASRIKVISRLNIAEKRVPQDGRMKLVLSKSRAIDFRVSTLPTLQGEKIVLRILDPASATLGIDALGYEPEQRAVLLDAIN
RPYGMVLVTGPTGSGKTVSLYTCLNILNKDGVNIATAEDPAEINLSGINQVNVDDRAGLTFPVALKAFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTLTRLMNMGVPTFNIASSILLITAQRLARRLCNCKQPMHVPEQALLDAG
FKPEDLDGSWTLYGPHGCERCKGSGYKGRVGIYQVMPVSEAMQRLIMSGATALDLANQARAEGVKDLRESGLLKVKQGLT
SLDEVLSTTNA

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=976444 AADV58_RS04585 WP_028994944.1 945393..947108(-) (pilB) [Azonexus hydrophilus strain NCP973]
ATGGCAGCAACTCCGCAAAACTCCCCGCTCAGCGGCCTCGCCCGCGCCCTTGTCCAGGCCGGCCGCCTGAAAGAAGGCGA
AGCCGAGGAGTTGGCACAACAGGCCCTGGCGGCGAAGTTGCCCCTGATCGAACAGATCATCGTTGCGAAAAAGGCGACCT
CGCTTGAAATTGCGCGCTTCTCGGCAGAAACCTTCGGTTATCCATTATTTGACCTCGATGCCATCGACGAACGTCACATT
CCTCAGGATGCCATTGACCGCAAGCTGATCGCCACCCACAAGGTCGTGCCGCTGAACAAGCGCGGCAATCGCCTCTCGGT
GGCCATCGCCGACCCAACCAACCTGCGGGCGCTCGATGAAATCCGCTTCCAGACCGGACTGACCGTCGATCCCATCGTCG
TCGAGCACCCCAAGTTACTACCGCTGGTTGCCAAATTCTCGGAATCGGCATCCGATGCGCTCAAAAATCTGGCCGACGAT
GACATCAACCTGGACTTTCTCGACGAGGAAGCCGCAGGAAAAACCGATGAAGCAAGCGCTCAGGAGATCGACGACGCACC
AGTCGTCAAGTTCATCCAGAAGATGTTGCTTGACGCCATCAACGACGGCGCTTCCGATATCCACTTCGAGCCTTACGAAA
AGTACTACCGGATCCGTTTCCGGGTCGACGGCATTCTCCGGGAAGTTGCGACACCACCATTGGCAATCAAGGAAAAGATC
GCCTCGCGGATCAAGGTCATCTCGCGCCTGAACATCGCCGAAAAGCGGGTCCCGCAGGATGGCCGGATGAAACTGGTGCT
ATCCAAGTCGCGGGCCATCGATTTCCGGGTCAGCACCCTGCCAACGCTGCAGGGCGAAAAAATCGTGCTGCGTATTCTTG
ATCCGGCCTCAGCCACGCTCGGCATCGATGCGCTTGGCTACGAACCCGAACAGAGGGCGGTCCTGCTCGACGCCATCAAC
CGCCCTTACGGCATGGTTCTGGTCACCGGCCCAACAGGTTCCGGCAAGACCGTTTCGCTCTACACCTGCCTGAACATCCT
CAATAAGGATGGCGTCAACATTGCCACGGCCGAGGATCCCGCAGAAATCAATCTGTCAGGCATCAATCAGGTCAACGTCG
ACGACCGCGCTGGCCTGACCTTCCCGGTGGCTTTGAAGGCCTTCCTGCGCCAGGATCCGGACATCATCATGGTTGGTGAA
ATCCGTGACCTGGAAACCGCAGAAATCGCCATCAAGGCAGCCCAGACCGGCCACATGGTGCTGTCCACGCTGCACACCAA
CGATGCGCCGCAGACATTGACCCGCCTGATGAATATGGGCGTACCGACCTTCAATATCGCCTCCAGCATCCTGCTGATCA
CCGCACAACGACTGGCCAGGCGCCTGTGCAACTGCAAGCAGCCCATGCATGTTCCCGAACAAGCCTTGCTCGACGCCGGC
TTCAAACCGGAGGACCTTGACGGCAGTTGGACGCTGTATGGCCCGCACGGCTGCGAACGCTGCAAAGGGAGCGGCTACAA
GGGACGGGTCGGCATCTATCAGGTCATGCCGGTCAGCGAAGCCATGCAGCGCCTGATCATGAGCGGGGCAACCGCTCTCG
ACCTGGCCAACCAGGCGCGCGCAGAAGGGGTCAAGGACCTGCGCGAATCCGGCCTGCTCAAAGTGAAACAAGGACTGACC
TCGCTCGATGAAGTGCTGAGCACGACGAACGCCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-149)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.101

100

0.543
  pilB Acinetobacter baylyi ADP1

54.021

100

0.541
  pilB Legionella pneumophila strain ERS1305867

53.59

100

0.536
  pilF Neisseria gonorrhoeae MS11

53.415

100

0.534
  pilB Vibrio cholerae strain A1552

49.453

95.972

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

47.695

98.774

0.471
  pilB Vibrio campbellii strain DS40M4

46.892

98.599

0.462
  pilF Thermus thermophilus HB27

38.316

100

0.391
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.927

90.718

0.371
  pilB/pilB1 Synechocystis sp. PCC 6803

39.736

92.995

0.37

Multiple sequence alignment