Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAC899_RS14540 Genome accession   NZ_CP151168
Coordinates   3086509..3088224 (+) Length   571 a.a.
NCBI ID   WP_317657727.1    Uniprot ID   -
Organism   Acinetobacter soli strain HY24     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3081509..3093224
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAC899_RS14505 (AAC899_14505) nusA 3081988..3083472 (-) 1485 WP_004943981.1 transcription termination factor NusA -
  AAC899_RS14510 (AAC899_14510) rimP 3083508..3084032 (-) 525 WP_004932478.1 ribosome maturation factor RimP -
  AAC899_RS14530 (AAC899_14530) secG 3085109..3085438 (-) 330 WP_004932477.1 preprotein translocase subunit SecG -
  AAC899_RS14535 (AAC899_14535) tpiA 3085448..3086245 (-) 798 WP_004932474.1 triose-phosphate isomerase -
  AAC899_RS14540 (AAC899_14540) pilB 3086509..3088224 (+) 1716 WP_317657727.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAC899_RS14545 (AAC899_14545) pilC 3088248..3089474 (+) 1227 WP_004932468.1 type II secretion system F family protein Machinery gene
  AAC899_RS14550 (AAC899_14550) pilD 3089474..3090334 (+) 861 WP_048765376.1 A24 family peptidase Machinery gene
  AAC899_RS14555 (AAC899_14555) coaE 3090337..3090936 (+) 600 WP_149929795.1 dephospho-CoA kinase -
  AAC899_RS14560 (AAC899_14560) - 3090912..3091841 (-) 930 WP_288408460.1 DMT family transporter -
  AAC899_RS14565 (AAC899_14565) rlmB 3091879..3092625 (-) 747 WP_004932455.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AAC899_RS14570 (AAC899_14570) - 3092727..3093053 (-) 327 WP_004932453.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 64115.76 Da        Isoelectric Point: 5.1033

>NTDB_id=975591 AAC899_RS14540 WP_317657727.1 3086509..3088224(+) (pilB) [Acinetobacter soli strain HY24]
MSLVTASPKFSGFIRRLVEEGHIDAHDMQMAQESAKKNKQDIVPYLIDHYAITPITIAETISVEFGEPLFDLDVFDPALI
LKDLIDEKLIRKYRVMPLVHRGHVLYVATCNPTNLEAMDAIRFNSKLKVEPIIVEHDKLERLLNEYFPEDSRFNFDTEDF
DLDLEVNTPASDDDDDEEISKDEAPVVKYINKLLIDAIRMNASDLHFEPYEKVYRVRYRVDGVLRLIATPPLQLATRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKTIDFRVNSLPTLFGEKIVLRILDPASAMLGIDALGYEPEQKALFTEALNRP
QGMLLITGPTGSGKTVSMYTGLNILNTEHANISTAEDPVEINLEGINQVNVNPKVGLTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHLVMSTLHTNNAAETLTRLRSMGVATFNIATSVNLVIAQRLARRLCNQCKRRIDVPRASLLEMGF
TETDLDAPDFQIYEPVGCKECREGYKGRVGIYEVMKITPEISRIIMQDGNALEIAAAAEQLGFSNLRRSGLKKVMQGVTS
LQEVNRVTNEE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=975591 AAC899_RS14540 WP_317657727.1 3086509..3088224(+) (pilB) [Acinetobacter soli strain HY24]
ATGTCATTAGTTACCGCATCACCTAAATTTTCAGGATTCATTCGGCGCCTTGTCGAAGAAGGTCATATTGATGCACACGA
CATGCAAATGGCTCAAGAGAGCGCAAAAAAAAACAAGCAAGATATTGTTCCTTATTTGATCGACCATTATGCCATTACCC
CGATCACCATTGCTGAAACCATCTCGGTCGAGTTTGGCGAACCGCTATTTGATCTGGATGTTTTTGATCCTGCGCTGATT
CTCAAAGATCTTATCGATGAAAAGCTGATTCGCAAGTATCGTGTAATGCCGTTGGTTCATCGTGGGCATGTGCTCTATGT
CGCGACCTGCAATCCGACCAATCTTGAAGCGATGGATGCCATCCGTTTTAACAGCAAACTCAAAGTCGAACCGATTATTG
TAGAACACGATAAGCTTGAGCGACTGTTAAACGAATATTTCCCCGAAGATAGCCGTTTTAACTTTGACACCGAAGATTTT
GATCTGGATCTGGAGGTGAATACCCCCGCTTCAGATGACGACGACGATGAAGAAATCAGTAAAGATGAAGCCCCGGTGGT
CAAATACATCAATAAATTACTGATTGATGCTATTCGTATGAATGCATCAGACTTACATTTTGAACCTTATGAAAAGGTAT
ATCGGGTCCGTTATCGGGTCGATGGTGTACTTCGTTTAATTGCAACACCACCCTTACAACTGGCTACCCGACTGGCCTCG
CGTTTGAAAGTGATGTCTCAAATGGATATTTCGGAAAAGCGTGTTCCGCAAGATGGTCGTATTAAACTGAAACTGTCGAA
AACCAAAACCATCGATTTTCGGGTCAACTCCCTACCGACCCTATTTGGTGAAAAGATTGTACTGCGGATTCTGGATCCTG
CGAGCGCGATGCTGGGTATCGATGCACTGGGCTACGAGCCTGAGCAAAAAGCATTGTTTACCGAAGCGCTGAATCGACCA
CAAGGCATGCTGCTGATTACCGGCCCTACAGGCTCGGGGAAAACCGTATCGATGTATACCGGCTTAAATATTCTCAATAC
CGAACATGCCAATATTTCTACCGCCGAAGATCCAGTCGAGATTAATCTTGAAGGTATTAATCAGGTCAATGTAAATCCCA
AAGTCGGCCTGACCTTTGCTGCGGCATTACGCTCTTTTTTACGTCAAGACCCTGATATCATTATGGTGGGGGAAATTCGT
GATTTAGAAACCGCAGAGATTGCCATTAAAGCGGCTCAAACCGGTCACTTGGTGATGTCGACACTGCATACCAATAACGC
TGCCGAAACACTGACACGCCTGAGAAGCATGGGGGTGGCGACCTTTAATATCGCCACCTCGGTCAATCTGGTGATTGCGC
AGCGCCTTGCCAGACGCCTGTGTAATCAGTGTAAACGGCGTATCGACGTGCCACGTGCCAGTTTACTCGAAATGGGATTT
ACCGAAACCGATTTAGATGCTCCAGACTTTCAGATCTATGAACCGGTGGGGTGTAAAGAATGTCGGGAGGGCTACAAAGG
GCGTGTGGGCATTTATGAAGTCATGAAAATTACACCGGAAATCTCAAGAATTATTATGCAAGACGGCAATGCACTTGAAA
TTGCAGCAGCCGCCGAACAGCTTGGATTTAGCAACTTACGCCGTTCTGGCTTAAAAAAAGTGATGCAGGGGGTCACATCG
CTACAGGAAGTCAATCGTGTGACGAATGAAGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-150)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

86.316

99.825

0.862
  pilB Acinetobacter baumannii D1279779

77.719

99.825

0.776
  pilB Legionella pneumophila strain ERS1305867

51.478

100

0.518
  pilF Neisseria gonorrhoeae MS11

50

98.424

0.492
  pilB Vibrio cholerae strain A1552

52.031

90.543

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

50.962

91.068

0.464
  pilB Vibrio campbellii strain DS40M4

50.29

90.543

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.378
  pilF Thermus thermophilus HB27

37.95

97.373

0.37

Multiple sequence alignment