Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WOB24_RS16350 Genome accession   NZ_CP150891
Coordinates   3504335..3505480 (-) Length   381 a.a.
NCBI ID   WP_339080354.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3499335..3510480
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOB24_RS16330 (WOB24_16330) - 3500302..3501573 (+) 1272 WP_341303951.1 dihydroorotase -
  WOB24_RS16335 (WOB24_16335) - 3501722..3502120 (-) 399 WP_341303952.1 TM2 domain-containing protein -
  WOB24_RS16340 (WOB24_16340) - 3502280..3503491 (+) 1212 WP_339080352.1 putative DNA modification/repair radical SAM protein -
  WOB24_RS16345 (WOB24_16345) - 3503491..3504369 (+) 879 WP_341303953.1 TIGR03915 family putative DNA repair protein -
  WOB24_RS16350 (WOB24_16350) pilU 3504335..3505480 (-) 1146 WP_339080354.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WOB24_RS16355 (WOB24_16355) pilT 3505586..3506620 (-) 1035 WP_339080355.1 type IV pilus twitching motility protein PilT Machinery gene
  WOB24_RS16360 (WOB24_16360) - 3506684..3507373 (+) 690 WP_339080356.1 YggS family pyridoxal phosphate-dependent enzyme -
  WOB24_RS16365 (WOB24_16365) proC 3507406..3508239 (+) 834 WP_339080357.1 pyrroline-5-carboxylate reductase -
  WOB24_RS16370 (WOB24_16370) - 3508249..3508839 (+) 591 WP_339080358.1 YggT family protein -
  WOB24_RS16375 (WOB24_16375) - 3508853..3509149 (+) 297 WP_341303954.1 DUF167 family protein -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 42585.79 Da        Isoelectric Point: 6.7257

>NTDB_id=973538 WOB24_RS16350 WP_339080354.1 3504335..3505480(-) (pilU) [Pseudomonas sp. TMP25]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGKIMPITKTPMSPEQTRETVHSVMNEQQRRDFTENHECNFAISARGVGR
FRVSAFYQRNLAGMVLRRIETNIPTLDELKLPEILKKLALTKRGLVLFVGATGTGKSTSLAAMIGYRNKNSSGHIISIED
PIEYIHQHQSCIVTQREVGIDTESFEVALKNTLRQAPDVILIGEVRTRETMDHAVAFAETGHLCLATLHANNANQALDRI
INFFPADRQNQVWMDLSLNLKAIVAQQLIPTPDGKGRRAVIEVLINTPLASDLIRKGEVHELKALMKRSTEQGMQTFDQA
LYNLYSHGEITYEDALLYADSANDLRLMIKLGSETDGDHLSSMAQGLSLEISDDDPARRFR

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=973538 WOB24_RS16350 WP_339080354.1 3504335..3505480(-) (pilU) [Pseudomonas sp. TMP25]
ATGGAATTCGAAAAACTGCTGCGCCTGATGGTCGAAAAGGGCGGCTCAGATCTGTTTATTACCGCTGGCGTGCCGCCGTC
GATGAAGGTCAACGGCAAGATCATGCCGATCACCAAGACGCCCATGTCGCCTGAGCAAACCCGTGAAACCGTGCACTCTG
TGATGAACGAGCAGCAACGGCGCGACTTTACTGAAAACCATGAGTGCAACTTCGCCATCAGTGCTCGCGGTGTGGGTCGT
TTCCGCGTAAGTGCCTTCTATCAGCGCAATCTGGCTGGCATGGTGCTGCGCCGTATCGAAACCAATATCCCGACTTTAGA
TGAGCTGAAGCTACCGGAAATTCTCAAGAAACTGGCCCTAACCAAGCGCGGCCTGGTGTTGTTTGTGGGAGCAACCGGTA
CCGGTAAGTCGACCTCGCTGGCGGCGATGATTGGCTACCGCAATAAGAACAGCAGCGGCCACATCATCTCCATCGAAGAC
CCCATCGAATACATCCACCAGCACCAGAGCTGCATCGTCACGCAGCGTGAGGTGGGTATCGACACCGAGTCCTTCGAAGT
GGCGCTAAAGAACACCCTGCGCCAAGCGCCAGACGTTATTTTGATCGGTGAGGTGCGCACCCGCGAAACCATGGACCACG
CTGTGGCCTTTGCCGAAACGGGCCACCTGTGTCTGGCCACGCTGCACGCCAACAACGCTAACCAAGCGCTGGACCGCATC
ATCAACTTCTTCCCGGCTGATCGACAAAATCAGGTGTGGATGGACTTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
GCTCATCCCGACGCCAGATGGCAAAGGCCGTCGTGCAGTTATTGAGGTGTTGATCAACACCCCACTGGCTTCCGACTTGA
TCCGCAAAGGTGAGGTGCATGAACTCAAGGCGCTGATGAAGCGCTCCACTGAGCAGGGCATGCAGACCTTTGATCAGGCC
CTGTACAACCTTTACAGCCATGGCGAGATCACCTACGAAGACGCGCTGCTGTATGCCGATTCGGCTAACGATCTGCGCCT
AATGATTAAGCTCGGCTCAGAAACCGATGGCGATCACCTCAGTAGCATGGCGCAAGGCTTGTCGCTGGAGATCAGTGATG
ATGATCCGGCCCGACGTTTTCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

91.601

100

0.916
  pilU Acinetobacter baylyi ADP1

64.986

93.701

0.609
  pilU Vibrio cholerae strain A1552

58.857

91.864

0.541
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.75

88.189

0.386
  pilT Pseudomonas aeruginosa PAK

41.493

87.927

0.365
  pilT Neisseria gonorrhoeae MS11

41.493

87.927

0.365
  pilT Neisseria meningitidis 8013

41.493

87.927

0.365

Multiple sequence alignment