Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WOB24_RS16355 Genome accession   NZ_CP150891
Coordinates   3505586..3506620 (-) Length   344 a.a.
NCBI ID   WP_339080355.1    Uniprot ID   -
Organism   Pseudomonas sp. TMP25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3500586..3511620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WOB24_RS16335 (WOB24_16335) - 3501722..3502120 (-) 399 WP_341303952.1 TM2 domain-containing protein -
  WOB24_RS16340 (WOB24_16340) - 3502280..3503491 (+) 1212 WP_339080352.1 putative DNA modification/repair radical SAM protein -
  WOB24_RS16345 (WOB24_16345) - 3503491..3504369 (+) 879 WP_341303953.1 TIGR03915 family putative DNA repair protein -
  WOB24_RS16350 (WOB24_16350) pilU 3504335..3505480 (-) 1146 WP_339080354.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WOB24_RS16355 (WOB24_16355) pilT 3505586..3506620 (-) 1035 WP_339080355.1 type IV pilus twitching motility protein PilT Machinery gene
  WOB24_RS16360 (WOB24_16360) - 3506684..3507373 (+) 690 WP_339080356.1 YggS family pyridoxal phosphate-dependent enzyme -
  WOB24_RS16365 (WOB24_16365) proC 3507406..3508239 (+) 834 WP_339080357.1 pyrroline-5-carboxylate reductase -
  WOB24_RS16370 (WOB24_16370) - 3508249..3508839 (+) 591 WP_339080358.1 YggT family protein -
  WOB24_RS16375 (WOB24_16375) - 3508853..3509149 (+) 297 WP_341303954.1 DUF167 family protein -
  WOB24_RS16380 (WOB24_16380) - 3509381..3511363 (+) 1983 WP_341303955.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37967.72 Da        Isoelectric Point: 6.8361

>NTDB_id=973539 WOB24_RS16355 WP_339080355.1 3505586..3506620(-) (pilT) [Pseudomonas sp. TMP25]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGPGAVFRTIPSKVLSMEDLGMGEIFRKISDVPRGLVLVTGPTGSGKSTTLAAMLDYINSNKYHHILTVED
PIEFVHESKKCLINQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQSVISQTLLKKVGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGSLGMQTLDSCL
KTLLSKGIVSREAAREKAKIPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=973539 WOB24_RS16355 WP_339080355.1 3505586..3506620(-) (pilT) [Pseudomonas sp. TMP25]
ATGGATATCACTGAATTGCTGGCCTTCAGCGCCAAGCAAGGTGCATCGGACCTGCACCTCTCCGCTGGCCTGCCTCCGAT
GATTCGGGTCGACGGTGATGTTCGCCGGATCAATTTGCCTGCACTCGACCATAAACAAGTGCATGCACTGATCTACGACA
TCATGAACGACAAGCAGCGTAAGGACTTCGAGGAGTTCCTCGAAACCGACTTCTCCTTTGAAGTGCCGGGTGTGGCGCGC
TTTCGGGTTAACGCCTTTAACCAAAACCGTGGCCCTGGCGCCGTGTTCCGGACCATTCCGTCGAAAGTGCTGAGCATGGA
AGACCTCGGCATGGGCGAGATTTTCCGTAAGATTTCTGATGTGCCGCGCGGTTTAGTGTTGGTTACCGGGCCGACTGGTT
CCGGTAAGTCGACCACGTTGGCGGCGATGCTCGACTATATAAACAGCAACAAATATCACCACATCCTGACGGTTGAAGAC
CCGATCGAGTTCGTTCACGAATCGAAAAAATGCCTGATCAACCAACGTGAAGTCCACCGCGACACCCTTGGCTTCTCTGA
AGCCCTGCGCTCAGCGCTGCGCGAAGATCCGGACATCATTCTGGTGGGCGAGATGCGCGACTTGGAAACCATCCGCTTGG
CCCTGACGGCTGCAGAAACGGGGCACTTGGTCTTTGGCACCTTGCACACCACCTCGGCGGCTAAAACCATCGACCGCGTA
GTGGACGTATTCCCGGCTGAGGAAAAGTCCATGGTGCGCTCCATGCTTTCTGAATCCTTGCAGTCAGTGATCTCGCAAAC
CCTGCTGAAGAAGGTTGGCGGCGGTCGCGTGGCAGCCCACGAAATCATGATCGGCACTCCGGCCATTCGTAACTTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCAGCGATTCAGACCGGCGGCTCGCTGGGCATGCAAACGCTCGACTCGTGCCTG
AAAACCTTGCTGTCCAAAGGCATCGTCAGCCGTGAAGCGGCACGCGAGAAAGCTAAAATACCGGAAAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.895

100

0.939
  pilT Pseudomonas aeruginosa PAK

93.023

100

0.93
  pilT Acinetobacter nosocomialis M2

84.884

100

0.849
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

75.148

98.256

0.738
  pilT Legionella pneumophila strain ERS1305867

75.148

98.256

0.738
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.235

98.837

0.674
  pilT Vibrio cholerae strain A1552

68.235

98.837

0.674
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Neisseria meningitidis 8013

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

100

0.515
  pilU Vibrio cholerae strain A1552

40.708

98.547

0.401
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

39.403

97.384

0.384

Multiple sequence alignment