Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WMZ02_RS13335 Genome accession   NZ_CP150864
Coordinates   2857687..2859372 (+) Length   561 a.a.
NCBI ID   WP_346338042.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain NJIFDCVp7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2852687..2864372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WMZ02_RS13315 (WMZ02_13315) pdhR 2854293..2855060 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WMZ02_RS13320 (WMZ02_13320) ampD 2855466..2856017 (-) 552 WP_005462574.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WMZ02_RS13325 (WMZ02_13325) nadC 2856110..2856997 (+) 888 WP_025442237.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WMZ02_RS13330 (WMZ02_13330) pilA 2857262..2857681 (+) 420 WP_025442238.1 pilin Machinery gene
  WMZ02_RS13335 (WMZ02_13335) pilB 2857687..2859372 (+) 1686 WP_346338042.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WMZ02_RS13340 (WMZ02_13340) pilC 2859396..2860619 (+) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  WMZ02_RS13345 (WMZ02_13345) pilD 2860684..2861553 (+) 870 WP_346338043.1 A24 family peptidase Machinery gene
  WMZ02_RS13350 (WMZ02_13350) coaE 2861554..2862168 (+) 615 WP_025633754.1 dephospho-CoA kinase -
  WMZ02_RS13355 (WMZ02_13355) zapD 2862196..2862936 (+) 741 WP_015297254.1 cell division protein ZapD -
  WMZ02_RS13360 (WMZ02_13360) yacG 2863005..2863199 (+) 195 WP_005462546.1 DNA gyrase inhibitor YacG -
  WMZ02_RS13365 (WMZ02_13365) rplS 2863609..2863962 (-) 354 WP_005462554.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62400.30 Da        Isoelectric Point: 5.5393

>NTDB_id=973175 WMZ02_RS13335 WP_346338042.1 2857687..2859372(+) (pilB) [Vibrio parahaemolyticus strain NJIFDCVp7]
MLTNLSTVLRQKGLLTFSQEESLIEQVKASGISMPEALLSSGFFTSSELAEHLSSMFGLSQPELSQYEYASLCQQLGLRE
LITRHNALPLHRTPSTLLLAVADPTNQQAEDDFRFATGLQVELVLVDFRELSAAIRRLYGRAIGQERSQLKEINQEELAS
LVDVGANEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLEALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETIIRLSNMGVESFNLASSLSLIIAQRLARKLCPYCKQPQEHTVQLQHLGIQTTDN
IFRANPDGCNECTHGYSGRTGIYEVMRFDESLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=973175 WMZ02_RS13335 WP_346338042.1 2857687..2859372(+) (pilB) [Vibrio parahaemolyticus strain NJIFDCVp7]
ATGTTAACAAACCTCTCAACGGTTCTTCGTCAAAAAGGATTATTGACGTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
TAAAGCTTCTGGCATCTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACATCAAGCGAACTGGCCGAGCACTTAA
GTTCTATGTTTGGCTTAAGTCAGCCCGAGTTATCTCAATATGAATATGCTTCTCTTTGCCAACAGCTCGGCCTACGTGAA
TTAATCACACGACATAATGCACTCCCACTCCACCGTACTCCTTCAACTTTATTATTAGCGGTTGCCGACCCCACGAATCA
ACAAGCTGAAGATGATTTCCGCTTTGCCACTGGTTTGCAGGTCGAACTGGTTCTGGTCGATTTTCGCGAACTAAGTGCCG
CAATTCGTCGCTTGTACGGGCGTGCCATTGGTCAGGAACGCTCCCAGCTCAAAGAGATCAACCAAGAAGAGCTAGCCAGC
TTAGTGGATGTCGGTGCAAATGAAATCGACAACATCGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTTCGTC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCGCAAGACGGGCGTATTAAGCTCAAGTTGAATCAAGACACAGCAAT
TGATATGCGTGTATCCACGCTACCTACTCTATTCGGAGAGAAAATCGTACTCCGACTGCTCGATAGCAGCTCTGCATCAC
TGGATATCGACAAGCTTGGTTATAGTGAGCAACAAAAACAACTGTATTTAGAGGCTCTGCGTCGTCCACAAGGTATGATT
CTGATGACAGGTCCAACAGGAAGCGGCAAAACCGTTTCGTTGTACACTGGGTTAAATATTCTCAATAAACCAGAGATCAA
CATCTCCACAGCGGAAGACCCGGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTCCAACCTAAGATTGGTTTTG
GCTTTGCAGAAGCTCTACGTTCTTTTCTGCGTCAAGATCCCGATGTGGTCATGGTGGGTGAGATTCGAGACCTAGATACT
GCGGAAATCGCGATTAAGGCGTCCCAAACCGGTCACTTAGTGCTCTCTACTTTGCATACCAACTCTGCGGCTGAAACCAT
CATTCGTTTATCAAACATGGGCGTAGAAAGTTTTAACCTCGCCTCTTCTCTTAGTTTGATTATTGCCCAACGCCTAGCTC
GCAAACTTTGTCCATATTGTAAACAACCGCAAGAGCATACCGTTCAACTTCAGCACCTCGGCATTCAAACAACTGACAAC
ATTTTTAGAGCCAATCCAGATGGTTGCAACGAATGCACCCATGGTTATTCTGGCCGAACAGGTATCTATGAAGTCATGCG
CTTTGATGAATCTCTATCCGAAGCTCTTATTAAAGGAGCCTCGGTACATGAACTGGAAAAGCTTGCCATTGCGAACGGCA
TGAGCACCTTGCAAATGTCCGGAATAGAAAAACTCAAACAAGGCATCACAAGCTTTAGTGAGTTACAACGCGTGCTCTAT
TTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

97.504

100

0.975
  pilB Vibrio campbellii strain DS40M4

90.553

100

0.906
  pilB Vibrio cholerae strain A1552

73.843

100

0.74
  pilB Acinetobacter baumannii D1279779

51.894

94.118

0.488
  pilB Acinetobacter baylyi ADP1

48.294

99.287

0.479
  pilB Legionella pneumophila strain ERS1305867

49.162

95.722

0.471
  pilF Neisseria gonorrhoeae MS11

45.035

100

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

37.563

100

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.861

100

0.373
  pilF Thermus thermophilus HB27

36.348

100

0.365

Multiple sequence alignment