Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VUK78_RS13650 Genome accession   NZ_CP150467
Coordinates   636149..637372 (-) Length   407 a.a.
NCBI ID   WP_005446256.1    Uniprot ID   K5TX94
Organism   Vibrio harveyi strain NH-LM1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 631149..642372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUK78_RS13615 (VUK78_13615) rimM 631287..631835 (+) 549 WP_005446245.1 ribosome maturation factor RimM -
  VUK78_RS13620 (VUK78_13620) trmD 631863..632606 (+) 744 WP_005446247.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  VUK78_RS13625 (VUK78_13625) rplS 632648..633001 (+) 354 WP_005379971.1 50S ribosomal protein L19 -
  VUK78_RS13630 (VUK78_13630) yacG 633567..633767 (-) 201 WP_026000212.1 DNA gyrase inhibitor YacG -
  VUK78_RS13635 (VUK78_13635) zapD 633824..634564 (-) 741 WP_005446251.1 cell division protein ZapD -
  VUK78_RS13640 (VUK78_13640) coaE 634592..635206 (-) 615 WP_017189130.1 dephospho-CoA kinase -
  VUK78_RS13645 (VUK78_13645) pilD 635207..636076 (-) 870 WP_005446255.1 A24 family peptidase Machinery gene
  VUK78_RS13650 (VUK78_13650) pilC 636149..637372 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  VUK78_RS13655 (VUK78_13655) pilB 637406..639091 (-) 1686 WP_340700209.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VUK78_RS13660 (VUK78_13660) - 639091..639531 (-) 441 WP_061065377.1 pilin -
  VUK78_RS13665 (VUK78_13665) nadC 639796..640683 (-) 888 WP_029790346.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VUK78_RS13670 (VUK78_13670) ampD 640776..641327 (+) 552 WP_017189135.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44945.20 Da        Isoelectric Point: 10.3829

>NTDB_id=970417 VUK78_RS13650 WP_005446256.1 636149..637372(-) (pilC) [Vibrio harveyi strain NH-LM1]
MKTIAPQLKNYRWKGINSSGKKTSGNVLAMTEVEVRERLDAQHIKIKKLKKGSISFITKISHRVKGKDITVFTRQIATML
MTGVPIVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASTHFDPLYTDLIATGEQSGNLSQVFERLATYREK
SEQLRAKVIKALIYPAMVVLVALGVSYLMLTKVIPEFEKMFTGFGAELPAFTQMVLNLSAWTQNWGPFIGLSFVSLLISG
KIMSQRSDSFRLLTARSSLRVPILGPVLSKAAIAKFSRTLATSFSAGIPILTALKTTSKTSGNMHYQLAIEEVYRDTAAG
MPMYIAMRNCNVFPELVLQMVMIGEESGRLDDMLNKIAAIYEFEVDNTVDNLSKILEPLIIVFLGVVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=970417 VUK78_RS13650 WP_005446256.1 636149..637372(-) (pilC) [Vibrio harveyi strain NH-LM1]
ATGAAAACCATCGCTCCACAACTTAAGAACTACCGTTGGAAAGGCATTAATAGCTCGGGAAAAAAAACCTCTGGTAACGT
GCTCGCAATGACAGAAGTCGAGGTAAGAGAGCGCCTTGATGCTCAGCACATCAAGATAAAAAAACTCAAAAAAGGCAGTA
TTTCTTTTATCACCAAGATAAGTCATCGAGTAAAAGGCAAAGACATTACGGTGTTTACTCGTCAAATCGCCACCATGTTG
ATGACTGGCGTCCCTATTGTTCAGGCACTCAAGTTAGTTTCAGAGAACCATAAAAAAGCTGAAATGAAATCGATCCTAAT
GAGTGTGACGCGAGCGGTAGAAGCAGGGACACCGATGTCGAAAGCCATGCGTACCGCTAGCACGCACTTTGATCCACTTT
ACACCGACTTAATCGCAACAGGTGAGCAGTCCGGTAACTTATCCCAGGTCTTTGAGCGTCTGGCGACCTACCGAGAAAAA
AGCGAGCAGTTGCGTGCAAAGGTTATTAAAGCGCTCATTTACCCGGCGATGGTGGTATTAGTAGCACTTGGGGTTTCTTA
TTTAATGCTGACAAAGGTTATTCCTGAGTTTGAAAAAATGTTTACAGGTTTTGGTGCCGAATTACCCGCCTTCACCCAGA
TGGTACTCAACCTTTCTGCTTGGACCCAAAATTGGGGTCCCTTCATTGGGCTGAGCTTTGTGAGCTTACTCATTTCTGGC
AAAATCATGTCACAACGCTCCGACTCATTTCGTCTTTTGACTGCACGGTCGAGTTTAAGAGTACCTATTTTAGGACCAGT
TTTATCCAAAGCTGCCATTGCGAAGTTTAGCCGTACTTTGGCGACCAGTTTTAGCGCAGGTATTCCTATCCTAACCGCCT
TGAAAACTACCTCAAAAACCTCTGGTAACATGCACTATCAATTGGCAATCGAAGAGGTATATCGAGATACTGCCGCTGGT
ATGCCAATGTACATTGCCATGCGTAACTGTAACGTCTTCCCAGAATTGGTATTGCAAATGGTCATGATTGGTGAAGAGTC
AGGTCGCTTAGATGACATGCTCAACAAGATAGCCGCTATTTATGAGTTCGAAGTCGATAACACCGTCGACAACCTAAGTA
AGATTTTGGAACCGTTGATCATTGTTTTTCTGGGTGTCGTTGTCGGTGGTTTGGTGACAGCAATGTACTTACCAATCTTT
AACTTGATGAGCGTATTGGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K5TX94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

98.28

100

0.983
  pilC Vibrio cholerae strain A1552

74

98.28

0.727
  pilC Acinetobacter baylyi ADP1

42.462

97.789

0.415
  pilC Pseudomonas stutzeri DSM 10701

41.414

97.297

0.403
  pilC Acinetobacter baumannii D1279779

40.447

99.017

0.4
  pilG Neisseria gonorrhoeae MS11

39.558

100

0.396
  pilC Legionella pneumophila strain ERS1305867

39.163

99.754

0.391
  pilG Neisseria meningitidis 44/76-A

38.725

100

0.388

Multiple sequence alignment