Detailed information NTG0970416

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: VUK78_RS13645
• Genome accession: NZ_CP150467
• Coordinates: 635207..636076 (-)
• Length: 289 a.a.
• NCBI ID: WP_005446255.1
• Uniprot ID: A0A3A1Q6Q3
• Organism: Vibrio harveyi strain NH-LM1
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 630207..641076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VUK78_RS13610 (VUK78_13610)	rpsP	631010..631258 (+)	249	WP_004410028.1	30S ribosomal protein S16	-
VUK78_RS13615 (VUK78_13615)	rimM	631287..631835 (+)	549	WP_005446245.1	ribosome maturation factor RimM	-
VUK78_RS13620 (VUK78_13620)	trmD	631863..632606 (+)	744	WP_005446247.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
VUK78_RS13625 (VUK78_13625)	rplS	632648..633001 (+)	354	WP_005379971.1	50S ribosomal protein L19	-
VUK78_RS13630 (VUK78_13630)	yacG	633567..633767 (-)	201	WP_026000212.1	DNA gyrase inhibitor YacG	-
VUK78_RS13635 (VUK78_13635)	zapD	633824..634564 (-)	741	WP_005446251.1	cell division protein ZapD	-
VUK78_RS13640 (VUK78_13640)	coaE	634592..635206 (-)	615	WP_017189130.1	dephospho-CoA kinase	-
VUK78_RS13645 (VUK78_13645)	pilD	635207..636076 (-)	870	WP_005446255.1	A24 family peptidase	Machinery gene
VUK78_RS13650 (VUK78_13650)	pilC	636149..637372 (-)	1224	WP_005446256.1	type II secretion system F family protein	Machinery gene
VUK78_RS13655 (VUK78_13655)	pilB	637406..639091 (-)	1686	WP_340700209.1	type IV-A pilus assembly ATPase PilB	Machinery gene
VUK78_RS13660 (VUK78_13660)	-	639091..639531 (-)	441	WP_061065377.1	pilin	-
VUK78_RS13665 (VUK78_13665)	nadC	639796..640683 (-)	888	WP_029790346.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32536.74 Da
• Isoelectric Point: 7.5422

>NTDB_id=970416 VUK78_RS13645 WP_005446255.1 635207..636076(-) (pilD) [Vibrio harveyi strain NH-LM1]
MEVFQYYPWLFVVFTAIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIEPPKETLTLSVPRSSCQQCGTHIRIR
DNIPVISWLLLKGKCHNCQSPISARYPLIELLTAFCSGFIAFHFGFSYFTVALVFFTFVLIAATFIDLDTMLLPDQLTLP
LMWAGIALALAGISPVSLQDSIIGAMAGYLCLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLIQLRLQKQGIEKAFPFGPYLAIAGWVSLIWGNQILNWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=970416 VUK78_RS13645 WP_005446255.1 635207..636076(-) (pilD) [Vibrio harveyi strain NH-LM1]
ATGGAAGTATTCCAGTACTACCCATGGCTATTCGTTGTATTTACCGCTATTTTTGGTTTGATCGTGGGTAGTTTCCTCAA
CGTAGTTATTTACCGTTTACCTAAGATCATGGAACTAGAATGGCGACGCGAGTGCGCTGAATCATTCCCTGAATACAAGA
TCGAGCCACCAAAAGAGACATTAACCTTAAGCGTGCCACGTTCATCTTGTCAGCAATGCGGTACACATATCCGCATTAGA
GACAATATCCCTGTGATTAGTTGGCTATTACTAAAAGGTAAATGCCACAACTGCCAATCACCTATCAGTGCCCGTTACCC
TCTTATCGAACTGCTTACCGCTTTTTGTTCGGGTTTTATCGCTTTTCATTTCGGTTTTAGCTACTTCACTGTGGCGCTGG
TTTTCTTTACCTTTGTATTGATTGCTGCCACATTTATCGACCTTGATACCATGTTGCTGCCAGACCAACTCACACTGCCA
TTGATGTGGGCGGGTATTGCTCTTGCTCTTGCCGGTATCAGCCCTGTGAGTCTTCAAGATTCTATTATTGGAGCAATGGC
AGGCTACTTATGTCTTTGGAGTGTTTATTGGTTATTCAAACTCCTCACAGGCAAAGAGGGAATGGGCTATGGTGACTTTA
AACTCCTTGCTGCATTAGGAGCTTGGCTGGGCTGGCAATCTCTGCCGATGATCATTTTACTTTCTTCGGTAGTAGGCGTC
ATTTTTGGTCTTATCCAGCTGCGCTTGCAAAAGCAAGGTATCGAAAAAGCCTTTCCTTTTGGTCCTTACCTTGCAATTGC
AGGATGGGTGAGCCTAATTTGGGGCAATCAAATCCTCAACTGGTACTTTACGTCGATTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A3A1Q6Q3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	97.232	100	0.972
pilD	Vibrio cholerae strain A1552	74.306	99.654	0.74
pilD	Acinetobacter baumannii D1279779	52.768	93.772	0.495
pilD	Acinetobacter nosocomialis M2	52.399	93.772	0.491
pilD	Neisseria gonorrhoeae MS11	45.652	95.502	0.436