Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   OO986_RS03100 Genome accession   NZ_AP026552
Coordinates   639310..640536 (-) Length   408 a.a.
NCBI ID   WP_045618629.1    Uniprot ID   A0AAN1PMC1
Organism   Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 634310..645536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO986_RS03060 (VV208B2_05360) rpsP 634498..634746 (+) 249 WP_011079541.1 30S ribosomal protein S16 -
  OO986_RS03065 (VV208B2_05370) rimM 634762..635310 (+) 549 WP_011079542.1 ribosome maturation factor RimM -
  OO986_RS03070 (VV208B2_05380) trmD 635338..636087 (+) 750 WP_011079543.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  OO986_RS03075 (VV208B2_05390) rplS 636129..636482 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  OO986_RS03080 (VV208B2_05400) yacG 636707..636901 (-) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  OO986_RS03085 (VV208B2_05410) zapD 636964..637704 (-) 741 WP_011079546.1 cell division protein ZapD -
  OO986_RS03090 (VV208B2_05420) coaE 637737..638345 (-) 609 WP_108695081.1 dephospho-CoA kinase -
  OO986_RS03095 (VV208B2_05430) pilD 638347..639216 (-) 870 WP_108695082.1 A24 family peptidase Machinery gene
  OO986_RS03100 (VV208B2_05440) pilC 639310..640536 (-) 1227 WP_045618629.1 type II secretion system F family protein Machinery gene
  OO986_RS03105 (VV208B2_05450) pilB 640585..642273 (-) 1689 WP_108695083.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OO986_RS03110 (VV208B2_05460) - 642290..642772 (-) 483 WP_011151128.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OO986_RS03115 (VV208B2_05470) nadC 642993..643880 (-) 888 WP_108695084.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OO986_RS03120 (VV208B2_05480) ampD 643973..644545 (+) 573 WP_108695085.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45253.53 Da        Isoelectric Point: 10.3244

>NTDB_id=96366 OO986_RS03100 WP_045618629.1 639310..640536(-) (pilC) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
MKLKTHSQIKNYQWKGINGAGKKVSGNTLALTELEVRDKLKMQHIQIKKIKKSSVSVFTRLSHKVKGKDITLLTRQLATM
LTTGVPILQSLKLVADNHRKAEMKSILANVSKAVEAGTPLSKALRSASPQFDGIYVDLVATGEQSGNLDQVFERLAFYRE
KNEQLRAKVIKALIYPCMVVLVALGVSYLMLTQVIPEFRKMFTGFGAELPWFTQKVLDLSDFVQAYSSWFFITLFSAIYL
LRGLSKKSPAFRLKMSRLSLKIPVIGGVLSKAAIAKFSRTLATSFSAGIPILTSLKTTSKTAGNLHYQYAIEDVYRDTAA
GMPMYIAMRNSLAFPELVLQMVMIGEESGQMDDMLNKIATIYEFEVDNTVDNLGKILEPLIIVFLGVVVGGLVTAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=96366 OO986_RS03100 WP_045618629.1 639310..640536(-) (pilC) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
ATGAAGCTTAAAACCCATTCACAAATCAAAAACTATCAATGGAAAGGGATTAACGGAGCTGGAAAAAAAGTCTCCGGCAA
CACGCTGGCGCTTACCGAGCTTGAGGTGCGTGATAAGCTGAAAATGCAGCATATCCAGATCAAAAAGATCAAGAAATCCA
GTGTGTCAGTCTTCACAAGGTTAAGCCATAAAGTCAAAGGTAAAGACATCACACTGCTTACCCGCCAACTCGCCACCATG
CTGACCACTGGTGTCCCTATTCTCCAATCACTCAAATTGGTTGCTGACAACCATCGCAAAGCCGAGATGAAGTCGATTTT
AGCCAATGTGAGCAAAGCCGTTGAAGCAGGCACGCCGTTATCCAAAGCGCTTCGCAGTGCCAGCCCACAATTCGATGGCA
TTTATGTTGACTTAGTCGCCACTGGTGAACAATCGGGTAATCTTGATCAAGTTTTTGAACGCCTCGCTTTTTATCGGGAA
AAAAACGAGCAGTTGCGAGCAAAAGTGATCAAGGCGCTGATTTATCCCTGCATGGTCGTGCTGGTCGCGTTGGGGGTATC
TTATTTAATGCTGACTCAGGTTATTCCCGAGTTTCGTAAAATGTTCACCGGATTCGGCGCAGAGCTGCCTTGGTTTACGC
AAAAAGTCTTAGACCTGTCTGATTTTGTGCAAGCCTACAGCAGTTGGTTTTTCATTACGTTGTTCAGTGCCATTTATTTA
CTTCGCGGTCTAAGCAAAAAATCACCCGCATTTCGCTTAAAAATGTCACGCTTGAGTTTAAAGATCCCTGTGATTGGAGG
GGTCTTATCCAAAGCAGCGATTGCCAAATTTAGCCGCACATTGGCCACCAGTTTCAGTGCAGGTATCCCGATCCTCACCA
GTTTAAAAACCACCAGTAAAACCGCTGGCAATCTGCATTACCAATATGCGATTGAAGATGTTTATCGTGACACCGCAGCC
GGGATGCCAATGTACATCGCCATGCGTAACAGCCTTGCTTTCCCAGAATTGGTACTGCAAATGGTGATGATTGGTGAAGA
ATCTGGCCAAATGGACGATATGCTCAACAAAATTGCCACCATCTATGAGTTTGAAGTGGATAACACCGTCGACAACCTCG
GTAAAATTCTCGAGCCGTTGATCATCGTCTTTCTGGGCGTTGTCGTCGGCGGATTGGTCACCGCAATGTACTTGCCGATC
TTTAATTTAATGAGTGTTCTGGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

78.5

98.039

0.77
  pilC Vibrio campbellii strain DS40M4

74.813

98.284

0.735
  pilC Pseudomonas stutzeri DSM 10701

41.337

99.02

0.409
  pilG Neisseria gonorrhoeae MS11

41.045

98.529

0.404
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402
  pilC Acinetobacter baumannii D1279779

39.756

100

0.4
  pilC Acinetobacter baylyi ADP1

40.602

97.794

0.397
  pilC Legionella pneumophila strain ERS1305867

40.099

99.02

0.397

Multiple sequence alignment