Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OO986_RS01720 Genome accession   NZ_AP026552
Coordinates   360798..362582 (+) Length   594 a.a.
NCBI ID   WP_108695110.1    Uniprot ID   -
Organism   Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 355798..367582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO986_RS01700 (VV208B2_03080) pilM 358112..359119 (+) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  OO986_RS01705 (VV208B2_03090) pilN 359109..359699 (+) 591 WP_045598453.1 PilN domain-containing protein Machinery gene
  OO986_RS01710 (VV208B2_03100) pilO 359692..360282 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  OO986_RS01715 (VV208B2_03110) pilP 360272..360784 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  OO986_RS01720 (VV208B2_03120) pilQ 360798..362582 (+) 1785 WP_108695110.1 type IV pilus secretin PilQ Machinery gene
  OO986_RS01725 (VV208B2_03130) aroK 362784..363302 (+) 519 WP_011079357.1 shikimate kinase AroK -
  OO986_RS01730 (VV208B2_03140) aroB 363335..364426 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -
  OO986_RS01735 (VV208B2_03150) - 364477..365973 (+) 1497 WP_011079359.1 AAA family ATPase -
  OO986_RS01740 (VV208B2_03160) - 366053..366880 (+) 828 WP_011079360.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64554.64 Da        Isoelectric Point: 4.5316

>NTDB_id=96355 OO986_RS01720 WP_108695110.1 360798..362582(+) (pilQ) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
MICELMMKNKIQLFPGMFSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGSEGGSASIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=96355 OO986_RS01720 WP_108695110.1 360798..362582(+) (pilQ) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGTTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACCTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAACGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACGGTGGTGGATTTGAAACCGGTAGCGGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTCAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTCTCGACCGTGGTTGAAAG
TATCGAAGTGTTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTTAGTGGTGAATTTCGTCACGACTATCGTCTGT
CTGGACGCTATCTCGAGGTGACAATTTCTCGCATCAAATCGGAACAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGTTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAATGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCCGTGACTGGTAATGTGACGTTGAGATTGGATGGCGTACCTTGGCAGCAAGTGCTCGATATCA
TTCTTCAAGTGAAAGGTTTAGATAAACGTGTTGAAGGCAATGTTGTGCTCATCGCTCCTCAAGATGAATTGGATTTGAGA
GAGAAGCAGCAGCTTGAGAAAGAGCGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATCAAAGTCAACTTTGC
CAAAGCGTCAGACATCGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCGCGAATTGTTACGGTGAACGAAGGCAATCTTGATGAGCTTGGTGTACGTTG
GGGTTTCACCTCCATCAACGGCAGCACAACTGTCGGTGGTTCAATCGAAAACAACCTTGCAACCATTGGTCTCTACAATG
CAGGTAGTGGTAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGCAGTGAAGGCGGTTCGGCCTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACCTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAACTCTCTGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACGGAGATCCCTTATCTAGAGTCTTCCTCCAGTGGAGCTACCACGGTTTCATTT
AAGAAAGCCGTGCTTAGCCTCAAAGTGACCCCACAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCCGGCGAAATTGTCAAAACAGGCACTGGAGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGTATTACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCTTTATTGGGGGCGCTTTTCCGCCGTAGCTATGAAAACGTGGGTAAACGTGAATTGCTGATATTTGT
CACACCTAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.797

99.327

0.773
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.514

99.158

0.729
  pilQ Vibrio cholerae strain A1552

73.514

99.158

0.729
  pilQ Legionella pneumophila strain ERS1305867

41.429

94.276

0.391
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

39.539

94.949

0.375
  comQ Acinetobacter baylyi ADP1

37.162

99.663

0.37

Multiple sequence alignment