Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   OO986_RS01705 Genome accession   NZ_AP026552
Coordinates   359109..359699 (+) Length   196 a.a.
NCBI ID   WP_045598453.1    Uniprot ID   -
Organism   Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 354109..364699
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OO986_RS01690 (VV208B2_03060) oxyR 354489..355388 (+) 900 WP_011079350.1 DNA-binding transcriptional regulator OxyR -
  OO986_RS01695 (VV208B2_03070) - 355459..357972 (-) 2514 WP_108695087.1 penicillin-binding protein 1A -
  OO986_RS01700 (VV208B2_03080) pilM 358112..359119 (+) 1008 WP_011151279.1 type IV pilus assembly protein PilM Machinery gene
  OO986_RS01705 (VV208B2_03090) pilN 359109..359699 (+) 591 WP_045598453.1 PilN domain-containing protein Machinery gene
  OO986_RS01710 (VV208B2_03100) pilO 359692..360282 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  OO986_RS01715 (VV208B2_03110) pilP 360272..360784 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  OO986_RS01720 (VV208B2_03120) pilQ 360798..362582 (+) 1785 WP_108695110.1 type IV pilus secretin PilQ Machinery gene
  OO986_RS01725 (VV208B2_03130) aroK 362784..363302 (+) 519 WP_011079357.1 shikimate kinase AroK -
  OO986_RS01730 (VV208B2_03140) aroB 363335..364426 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22658.89 Da        Isoelectric Point: 7.6440

>NTDB_id=96352 OO986_RS01705 WP_045598453.1 359109..359699(+) (pilN) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
MQHKINLLPWREHKREEHKRRFVSLVALGALIAVGIQWGIGQYFSYQQRLQQERLDYLTHYIAQLDQRIQAMNIAKEEHS
QILARLKVVESLQAERNKTTAFMNLMTELIPEGVYVDKIKMNTNDVELAGISDSTARLATMLDNLERSTFLKDVEMHSIV
HDKKRFGKSFQTFTVSFVFDEHADSANTLAKEGNHG

Nucleotide


Download         Length: 591 bp        

>NTDB_id=96352 OO986_RS01705 WP_045598453.1 359109..359699(+) (pilN) [Vibrio vulnificus strain VV20-8B-2 isolate homogenised shrimp]
ATGCAGCATAAGATCAACCTGTTACCTTGGCGTGAACATAAGCGTGAGGAGCATAAACGTCGTTTTGTCAGTCTGGTCGC
ATTAGGTGCCCTGATCGCGGTCGGTATTCAGTGGGGCATTGGCCAGTACTTTTCATACCAACAACGTTTGCAGCAAGAGC
GTCTCGATTATCTTACCCACTATATCGCACAGTTGGATCAGCGTATCCAAGCGATGAACATTGCCAAGGAAGAGCATAGC
CAGATCCTCGCTCGACTCAAGGTTGTCGAGTCGCTTCAAGCGGAACGCAACAAAACCACCGCATTTATGAACTTGATGAC
GGAACTGATCCCTGAGGGGGTGTATGTCGACAAGATCAAAATGAACACCAACGACGTTGAGTTGGCTGGCATAAGCGATA
GTACCGCTCGACTCGCCACCATGTTGGATAATTTGGAACGTTCCACCTTTCTAAAAGACGTGGAAATGCATTCGATTGTT
CATGACAAGAAGCGTTTTGGTAAGAGCTTCCAGACGTTTACCGTTTCTTTTGTGTTTGATGAACATGCCGATTCTGCCAA
TACGTTGGCCAAGGAGGGGAATCATGGCTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

68.508

92.347

0.633
  pilN Vibrio cholerae strain A1552

63.842

90.306

0.577

Multiple sequence alignment