Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WDB71_RS01800 Genome accession   NZ_CP149925
Coordinates   348402..350117 (-) Length   571 a.a.
NCBI ID   WP_341502943.1    Uniprot ID   -
Organism   Gallaecimonas sp. GXIMD4217     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343402..355117
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDB71_RS01770 (WDB71_01770) mutT 344314..344715 (+) 402 WP_341502938.1 8-oxo-dGTP diphosphatase MutT -
  WDB71_RS01775 (WDB71_01775) yacG 344712..344900 (-) 189 WP_341502939.1 DNA gyrase inhibitor YacG -
  WDB71_RS01780 (WDB71_01780) zapD 344911..345636 (-) 726 WP_341502940.1 cell division protein ZapD -
  WDB71_RS01785 (WDB71_01785) coaE 345667..346278 (-) 612 WP_341504169.1 dephospho-CoA kinase -
  WDB71_RS01790 (WDB71_01790) pilD 346275..347135 (-) 861 WP_341502941.1 A24 family peptidase Machinery gene
  WDB71_RS01795 (WDB71_01795) pilC 347142..348392 (-) 1251 WP_341502942.1 type II secretion system F family protein Machinery gene
  WDB71_RS01800 (WDB71_01800) pilB 348402..350117 (-) 1716 WP_341502943.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WDB71_RS01805 (WDB71_01805) - 350323..350742 (-) 420 WP_341502944.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WDB71_RS01810 (WDB71_01810) nadC 350880..351731 (-) 852 WP_341502945.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WDB71_RS01815 (WDB71_01815) ampD 351855..352394 (+) 540 WP_341502946.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WDB71_RS01820 (WDB71_01820) ampE 352391..353200 (+) 810 WP_341502947.1 regulatory signaling modulator protein AmpE -
  WDB71_RS01825 (WDB71_01825) pdhR 353330..354103 (+) 774 WP_341502948.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63049.64 Da        Isoelectric Point: 5.4928

>NTDB_id=962214 WDB71_RS01800 WP_341502943.1 348402..350117(-) (pilB) [Gallaecimonas sp. GXIMD4217]
MSGFLPLAKGTLALLLRRGKIDQSVAEELLKDNNSGLLPLFDVLKKSELSAADFARFLSEQYGLPLFDLAKFDIDNIPST
LINEQLINKHFVLPLFKRQNRIFIAIADPSNQAALEDFRFNLRLPTEPVLVNADELAKAIDKLLEKSESATLDVDVQDSE
LADLVVEDEDDKDQDLVGVGGKDEDAPIVIYINKILMDAIRKGASDLHFEPYEKSYRVRFRIDGILHEVAAPPAALSGRL
AARIKVMAKLDIAERRIPQDGRIKLRLSRRKAIDFRVSTLPTLWGEKIVMRILDSSSAQLGIDMLGYEPEQKRLYMESLQ
KPQGMILVTGPTGSGKTVSLYTGLNILNTQERNISTAEDPVEINLPGINQVQINMKAGMDFASALRAFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSSSETLTRLMNMGVPSYNIASSVTLVIAQRLARRLCSSCKAPEQLPKDELLRQ
GYAPAQIDEGFTLYKPVGCKECTGGYKGRVGIYEAMPMTEAIAKIIMEGGNSLDIAAQAQRDGIKNLRQSGLEKARQGVT
SLAEINRVTSY

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=962214 WDB71_RS01800 WP_341502943.1 348402..350117(-) (pilB) [Gallaecimonas sp. GXIMD4217]
ATGTCAGGCTTCCTACCCCTCGCCAAAGGCACCCTGGCGCTGCTGCTCAGGCGCGGCAAGATAGACCAGAGCGTGGCCGA
AGAGCTGCTCAAGGACAACAATTCCGGCCTACTGCCGCTGTTCGACGTGCTGAAGAAGTCCGAACTCAGCGCCGCCGACT
TCGCCCGCTTCCTGTCGGAGCAATACGGCCTGCCCCTCTTTGACTTGGCCAAGTTCGATATCGACAATATTCCCTCAACA
CTCATTAATGAGCAGTTGATCAACAAGCATTTCGTTCTGCCGCTGTTCAAGCGCCAGAACCGCATCTTTATCGCCATCGC
CGACCCCAGCAACCAGGCGGCGTTGGAGGACTTCCGCTTCAACCTGCGCCTGCCCACAGAGCCCGTGCTGGTGAACGCCG
ATGAGCTGGCCAAGGCCATAGACAAGCTGCTGGAGAAAAGCGAATCGGCCACCCTGGATGTGGATGTCCAGGACTCCGAG
CTGGCAGACCTGGTCGTCGAAGATGAGGACGACAAGGATCAGGATCTGGTCGGCGTTGGCGGCAAGGATGAAGATGCCCC
CATCGTCATCTATATCAACAAGATATTGATGGACGCCATCCGCAAGGGGGCGTCGGACCTTCATTTCGAGCCTTACGAAA
AGAGCTACCGGGTACGCTTTCGTATCGACGGCATACTCCATGAAGTGGCGGCTCCCCCCGCCGCCTTGTCCGGCCGCTTG
GCGGCGCGGATCAAGGTCATGGCCAAGCTGGATATCGCCGAGCGCCGTATCCCCCAGGATGGCCGTATCAAGCTGCGCCT
GAGCCGCCGCAAGGCCATCGACTTCCGCGTATCGACCCTGCCGACCCTGTGGGGCGAAAAGATCGTGATGCGTATCCTGG
ACTCCAGCAGCGCCCAACTGGGCATCGACATGCTGGGTTACGAGCCGGAGCAGAAACGTCTGTATATGGAATCTCTGCAA
AAGCCCCAGGGGATGATACTGGTAACGGGCCCTACCGGCTCGGGCAAGACGGTGTCCCTCTATACCGGTCTCAATATCCT
CAATACCCAGGAACGTAATATCTCTACCGCCGAAGATCCGGTGGAAATCAACCTGCCCGGCATCAACCAGGTGCAGATTA
ACATGAAGGCGGGCATGGATTTCGCCTCTGCTCTCAGGGCCTTCCTGCGTCAGGATCCCGACGTCATCATGGTGGGTGAG
ATACGGGATCTGGAAACCGCCGAGATAGCGATCAAGGCCGCCCAGACGGGTCACCTGGTGCTGTCCACCCTGCACACCAA
CTCCTCGTCCGAGACCCTGACCCGTCTCATGAACATGGGCGTGCCTTCCTATAACATCGCCTCTTCGGTGACCCTGGTCA
TCGCCCAGCGGCTGGCTCGGCGCCTTTGCAGCAGTTGCAAGGCCCCCGAACAACTCCCCAAGGACGAGCTGTTACGCCAA
GGCTATGCGCCGGCGCAGATCGACGAGGGCTTCACATTGTATAAGCCCGTTGGCTGTAAGGAATGTACCGGGGGTTACAA
AGGCAGGGTGGGCATCTATGAAGCCATGCCCATGACCGAAGCCATTGCCAAAATCATCATGGAAGGCGGAAACTCGTTGG
ATATTGCCGCCCAGGCACAACGGGATGGAATCAAGAACCTGCGCCAATCCGGTCTTGAAAAGGCCCGCCAGGGAGTCACC
TCACTGGCGGAGATCAACCGGGTGACCAGTTACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-148)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

56.326

100

0.569
  pilB Acinetobacter baumannii D1279779

59.924

92.644

0.555
  pilB Legionella pneumophila strain ERS1305867

55.477

99.124

0.55
  pilB Vibrio cholerae strain A1552

55.019

94.221

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

54.12

93.52

0.506
  pilB Vibrio campbellii strain DS40M4

53.745

93.52

0.503
  pilF Neisseria gonorrhoeae MS11

46.678

100

0.468
  pilF Thermus thermophilus HB27

39.367

99.65

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.455

96.322

0.38
  pilB/pilB1 Synechocystis sp. PCC 6803

41.326

89.842

0.371