Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   WIR66_RS16945 Genome accession   NZ_CP149679
Coordinates   3797193..3798071 (-) Length   292 a.a.
NCBI ID   WP_339080883.1    Uniprot ID   -
Organism   Clostridioides difficile strain Z1322PCD0052     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3792193..3803071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIR66_RS16925 (WIR66_16860) - 3792752..3793318 (-) 567 WP_003437877.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WIR66_RS16930 (WIR66_16865) pilO 3793345..3794253 (-) 909 WP_003437879.1 type 4a pilus biogenesis protein PilO -
  WIR66_RS16935 (WIR66_16870) - 3794246..3795958 (-) 1713 WP_003437881.1 PilN domain-containing protein -
  WIR66_RS16940 (WIR66_16875) - 3795973..3797181 (-) 1209 WP_021359936.1 type II secretion system F family protein -
  WIR66_RS16945 (WIR66_16880) pilF 3797193..3798071 (-) 879 WP_339080883.1 GspE/PulE family protein Machinery gene
  WIR66_RS16950 (WIR66_16885) - 3798100..3798867 (-) 768 WP_339080885.1 ATPase, T2SS/T4P/T4SS family -
  WIR66_RS16955 (WIR66_16890) - 3798996..3799511 (-) 516 WP_074078013.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WIR66_RS16960 (WIR66_16895) - 3800596..3801546 (-) 951 WP_003421448.1 ribose-phosphate pyrophosphokinase -
  WIR66_RS16965 (WIR66_16900) glmU 3801641..3803020 (-) 1380 WP_021359938.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -

Sequence

Protein


Download         Length: 292 a.a.        Molecular weight: 32408.42 Da        Isoelectric Point: 5.2792

>NTDB_id=960707 WIR66_RS16945 WP_339080883.1 3797193..3798071(-) (pilF) [Clostridioides difficile strain Z1322PCD0052]
MPTNRDEKIAMRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQLNTENRNLLTIED
PIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTETAEIAIRASLTGHLVLSTLHANTAVGAISRLLDI
DVESFLITSSVLGVISQRLTRKICEHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPE
IKELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD

Nucleotide


Download         Length: 879 bp        

>NTDB_id=960707 WIR66_RS16945 WP_339080883.1 3797193..3798071(-) (pilF) [Clostridioides difficile strain Z1322PCD0052]
ATGCCAACTAACAGGGATGAAAAGATAGCTATGAGAGTTCTTGATAAGAGTAATTTTATGGTTAGTAAAGAAGTACTAGG
AATAGACGAACATGGCTCTAAGATATATGATGAATTGATAAACACTCCTTATGGTCTTATATTGGTTGTTGGCCCTACTG
GAAGTGGGAAAACCACTACATTGTATTCAATGTTAAATCAACTAAATACAGAAAATAGAAATCTATTAACAATAGAAGAC
CCTATAGAGTATGAATTACCTGGTGTAAATCAATCTCAAATAAATGAAAAGGCAGGGCTTACTTTCGCCAGCGGACTTAG
AGCTTTTATGCGTCAAGACCCAGATATAATAATGGTTGGAGAGATAAGAGATACTGAGACAGCAGAAATAGCAATTAGAG
CTTCTCTTACAGGACATTTAGTTTTAAGTACATTACATGCAAATACAGCAGTTGGAGCAATTTCACGTCTTTTAGATATA
GATGTGGAAAGTTTTTTAATAACTTCATCTGTTTTAGGTGTAATATCTCAAAGACTTACACGAAAAATATGTGAGCATTG
TAAGGTTTCATATGAAGCAGATATTGGAGAAAAAAAGGCTCTTGGTATTGATGTAAATGAATCAGTAACTATTTATAGAG
GAAAAGGTTGTGAAAGGTGTAATAATACTGGATATAAAGGACGTCTAGGTATATTTGAAATGCTTGAAATTACTCCAGAA
ATAAAAGAGTTAATAGATTCATCTGCAAATCAAAGAGAAATTTTAAAGATGGCTAGAAAACAAGGTATGGTTAGCTTAAA
AGAGGATATAGTTAAAAAAGTTTTAAATGGAAAGACAACAGTAGAAGAAATGATTAGAATAATTTTGATGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(1-182)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Thermus thermophilus HB27

50.174

98.288

0.493
  pilB Legionella pneumophila strain ERS1305867

48.276

99.315

0.479
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.552

99.315

0.462
  pilB Acinetobacter baylyi ADP1

46.392

99.658

0.462
  pilB Acinetobacter baumannii D1279779

45.734

100

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

43.791

100

0.459
  pilF Neisseria gonorrhoeae MS11

46.181

98.63

0.455
  pilB Vibrio parahaemolyticus RIMD 2210633

44.097

98.63

0.435
  pilB Vibrio campbellii strain DS40M4

42.708

98.63

0.421
  pilB Vibrio cholerae strain A1552

42.361

98.63

0.418
  ctsE Campylobacter jejuni subsp. jejuni 81-176

40.278

98.63

0.397
  pilB Haemophilus influenzae 86-028NP

45.339

80.822

0.366