Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   WKI54_RS20460 Genome accession   NZ_CP149648
Coordinates   3970558..3971595 (-) Length   345 a.a.
NCBI ID   WP_014418377.1    Uniprot ID   -
Organism   Bacillus velezensis strain MJ04     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3965558..3976595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI54_RS20410 (WKI54_20410) - 3965791..3966576 (-) 786 WP_017418136.1 TasA family protein -
  WKI54_RS20415 (WKI54_20415) - 3966641..3967225 (-) 585 WP_012117977.1 signal peptidase I -
  WKI54_RS20420 (WKI54_20420) tapA 3967197..3967868 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  WKI54_RS20425 (WKI54_20425) - 3968127..3968456 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  WKI54_RS20430 (WKI54_20430) - 3968497..3968676 (-) 180 WP_003153093.1 YqzE family protein -
  WKI54_RS20435 (WKI54_20435) comGG 3968733..3969110 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  WKI54_RS20440 (WKI54_20440) comGF 3969111..3969506 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  WKI54_RS20445 (WKI54_20445) comGE 3969520..3969834 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  WKI54_RS20450 (WKI54_20450) comGD 3969818..3970255 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  WKI54_RS20455 (WKI54_20455) comGC 3970245..3970553 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  WKI54_RS20460 (WKI54_20460) comGB 3970558..3971595 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  WKI54_RS20465 (WKI54_20465) comGA 3971582..3972652 (-) 1071 WP_106067891.1 competence type IV pilus ATPase ComGA Machinery gene
  WKI54_RS20470 (WKI54_20470) - 3972844..3973794 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  WKI54_RS20475 (WKI54_20475) - 3973940..3975241 (+) 1302 WP_021494315.1 hemolysin family protein -
  WKI54_RS20480 (WKI54_20480) - 3975279..3976109 (-) 831 WP_017418143.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39600.91 Da        Isoelectric Point: 9.6787

>NTDB_id=960309 WKI54_RS20460 WP_014418377.1 3970558..3971595(-) (comGB) [Bacillus velezensis strain MJ04]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYQVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGELLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPCYEKDLAAAVSHGQANGRLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=960309 WKI54_RS20460 WP_014418377.1 3970558..3971595(-) (comGB) [Bacillus velezensis strain MJ04]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GACGGCTGAGGGAAGGTGATGCGTTTTACCAAGTGCTTGAGGCACTGTCTTTTCACAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGGGAATTGCCGGGGGCGATGAAACAAAGCGGCGAATTATTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TCATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACCTCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTGATTGTTGTGAAAATCCCCCTATTAGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTGCTATGAAAAAGACCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCGGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

66.254

93.623

0.62