Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   WKI54_RS20465 Genome accession   NZ_CP149648
Coordinates   3971582..3972652 (-) Length   356 a.a.
NCBI ID   WP_106067891.1    Uniprot ID   -
Organism   Bacillus velezensis strain MJ04     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3966582..3977652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKI54_RS20415 (WKI54_20415) - 3966641..3967225 (-) 585 WP_012117977.1 signal peptidase I -
  WKI54_RS20420 (WKI54_20420) tapA 3967197..3967868 (-) 672 WP_014418371.1 amyloid fiber anchoring/assembly protein TapA -
  WKI54_RS20425 (WKI54_20425) - 3968127..3968456 (+) 330 WP_012117979.1 DUF3889 domain-containing protein -
  WKI54_RS20430 (WKI54_20430) - 3968497..3968676 (-) 180 WP_003153093.1 YqzE family protein -
  WKI54_RS20435 (WKI54_20435) comGG 3968733..3969110 (-) 378 WP_017418138.1 competence type IV pilus minor pilin ComGG Machinery gene
  WKI54_RS20440 (WKI54_20440) comGF 3969111..3969506 (-) 396 WP_025649850.1 competence type IV pilus minor pilin ComGF -
  WKI54_RS20445 (WKI54_20445) comGE 3969520..3969834 (-) 315 WP_017418140.1 competence type IV pilus minor pilin ComGE Machinery gene
  WKI54_RS20450 (WKI54_20450) comGD 3969818..3970255 (-) 438 WP_007612572.1 competence type IV pilus minor pilin ComGD Machinery gene
  WKI54_RS20455 (WKI54_20455) comGC 3970245..3970553 (-) 309 WP_014418376.1 competence type IV pilus major pilin ComGC Machinery gene
  WKI54_RS20460 (WKI54_20460) comGB 3970558..3971595 (-) 1038 WP_014418377.1 competence type IV pilus assembly protein ComGB Machinery gene
  WKI54_RS20465 (WKI54_20465) comGA 3971582..3972652 (-) 1071 WP_106067891.1 competence type IV pilus ATPase ComGA Machinery gene
  WKI54_RS20470 (WKI54_20470) - 3972844..3973794 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  WKI54_RS20475 (WKI54_20475) - 3973940..3975241 (+) 1302 WP_021494315.1 hemolysin family protein -
  WKI54_RS20480 (WKI54_20480) - 3975279..3976109 (-) 831 WP_017418143.1 STAS domain-containing protein -
  WKI54_RS20490 (WKI54_20490) - 3976316..3976696 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  WKI54_RS20495 (WKI54_20495) - 3976933..3977178 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40557.90 Da        Isoelectric Point: 9.4729

>NTDB_id=960310 WKI54_RS20465 WP_106067891.1 3971582..3972652(-) (comGA) [Bacillus velezensis strain MJ04]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFRGAGAVLLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=960310 WKI54_RS20465 WP_106067891.1 3971582..3972652(-) (comGA) [Bacillus velezensis strain MJ04]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAATGAGGCCTATACAGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGATTCCCCCTCTGGATAAACTGTCTTTATTTCGGGGGGCGGGCGCCGTACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGACCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCCGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
TATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCTCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.022

100

0.82
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374