Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WE862_RS01980 Genome accession   NZ_CP149571
Coordinates   402387..404093 (+) Length   568 a.a.
NCBI ID   WP_033115377.1    Uniprot ID   -
Organism   Aeromonas jandaei strain DSM 7311     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 397387..409093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WE862_RS01955 (WE862_01955) pdhR 398565..399329 (-) 765 WP_033115373.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WE862_RS01960 (WE862_01960) ampD 399608..400177 (-) 570 WP_042033204.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WE862_RS01965 (WE862_01965) - 400301..400771 (+) 471 WP_042033202.1 retropepsin-like aspartic protease -
  WE862_RS01970 (WE862_01970) nadC 400775..401632 (+) 858 WP_042033200.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WE862_RS01975 (WE862_01975) - 401955..402383 (+) 429 WP_042033198.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WE862_RS01980 (WE862_01980) pilB 402387..404093 (+) 1707 WP_033115377.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WE862_RS01985 (WE862_01985) pilC 404239..405480 (+) 1242 WP_339058694.1 type II secretion system F family protein Machinery gene
  WE862_RS01990 (WE862_01990) pilD 405503..406375 (+) 873 WP_042033195.1 A24 family peptidase Machinery gene
  WE862_RS01995 (WE862_01995) coaE 406393..407001 (+) 609 WP_042033194.1 dephospho-CoA kinase -
  WE862_RS02000 (WE862_02000) zapD 407046..407768 (+) 723 WP_042033191.1 cell division protein ZapD -
  WE862_RS02005 (WE862_02005) yacG 407772..407972 (+) 201 WP_215805995.1 DNA gyrase inhibitor YacG -
  WE862_RS02010 (WE862_02010) mutT 408045..408449 (-) 405 WP_042033189.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62564.96 Da        Isoelectric Point: 5.3215

>NTDB_id=959884 WE862_RS01980 WP_033115377.1 402387..404093(+) (pilB) [Aeromonas jandaei strain DSM 7311]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILESKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDESSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYEQKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRMSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=959884 WE862_RS01980 WP_033115377.1 402387..404093(+) (pilB) [Aeromonas jandaei strain DSM 7311]
ATGACCTCTAGCCCCAACAGCGGCCTGGCATTAAGCCTGGCCGCCTCTTCACTGATAAGTGAGGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGTAAACCCTTTGTAACCTTCCTGATCGAGAACGAGATCCTTGAGAGCAAGGCTCTCG
CCGATTTTTGCGAACTGGAGTATGGGGTACCTCTGCTGGATCTTGCAGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAGCACCATGTGCTTCCCATATACACCCAAGGGCATACCCTCTATATCGCCATGTCTGA
CCCGACCAATGTGTCGGCACTGGAAGACTTCGGCTTCAGCTTCGGTCTCCATACCGAAGCGCTGCTGGTCGAGGAGAACA
AACTCACTGCGGCAATAGGAAAATTGCTGGAAAGTGAGAACGATGCTCTCGGAATGGAGGATATCGATGAATCTGAAATC
TCCGACCTCGAAGTATCCGATGAAAGCAGCAGGTTGGATGAGAGCGTCAATACCGCTGATGATGACGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGTGGTGCCTCCGACCTGCACTTTGAGCCCTACGAACAAAAATACC
GCATTCGCTTTCGCATCGACGGCATCCTGCATGAAGTGGCCACGCCGCCGGTCAATCTGGCCAACCGTTTTTCTGCCCGT
CTCAAAGTCATGGCGCGGCTCGATATCGCAGAGCGCCGGCTGCCGCAGGATGGCCGTATCAAGCTCAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACAATGTGGGGCGAGAAAATTGTTATTCGTCTGCTCGATTCATCAG
CTGCCCGTCTCAATATCGAGCAACTCGGCTTTGACGACCGGCAAAAGGCGCAATATCTACGGGCACTCTCTAAACCTCAA
GGCATGATCTTGGTCACCGGCCCGACCGGTTCGGGTAAAACAGTCTCGCTCTATACCGGTCTCAATATTCTCAACACTAC
CGAGGTGAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGTGTCAACCAGGTGCAGGTCAACCCCAAGG
CTGGGCTGACCTTTGCCAGCGCCCTGCGCTCCTTCTTGCGGCAAGACCCGGATGTGGTGATGGTGGGGGAGATCCGCGAT
CTGGAGACGGCAGAAATAGCCATCAAGGCCGCCCAGACCGGTCACCTGGTACTATCCACCCTGCACACCAACTCAGCCGC
CGAAACCCTAACCCGGATGATGAACATGGGAGTGCCAGCCTTTAATATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGCTGGCACGCAAACTGTGTGACCACTGCAAGGCTCCCGAAGTAGTGCCGGAAGCAGAGTTACTGGAGCTGGGTTTTACT
CAGCAGCAACTGGCGGCGGGGCTGCGGCTGTTCAAACCGGTCGGCTGCAAGGAGTGCTCTGGTGGATACAAGGGTCGGGT
CGGTATCTACGAGATCATGCTGATGTCGGAAAACATCGCAAAGCTGATCATGCAGGGGGCCAACTCGCTACAAATAGCGG
CCATAGCCCAGAAAGAGGGGATGCGTACCTTACGTATGTCAGGATTGGAAAAGGCTCGCCTTGGCGTCACCAGTCTGGCG
GAGGTCAACCGGGTGACAACGAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

50.871

100

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.241

99.296

0.509
  pilF Neisseria gonorrhoeae MS11

46.986

99.296

0.467
  pilB/pilB1 Synechocystis sp. PCC 6803

38.596

100

0.387
  pilF Thermus thermophilus HB27

40.341

92.958

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361