Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WIR04_RS02320 Genome accession   NZ_CP149130
Coordinates   488223..489929 (-) Length   568 a.a.
NCBI ID   WP_338890149.1    Uniprot ID   -
Organism   Aeromonas rivipollensis strain BE1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483223..494929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WIR04_RS02290 mutT 483819..484223 (+) 405 WP_025328450.1 8-oxo-dGTP diphosphatase MutT -
  WIR04_RS02295 yacG 484298..484492 (-) 195 WP_042053535.1 DNA gyrase inhibitor YacG -
  WIR04_RS02300 zapD 484505..485227 (-) 723 WP_338890141.1 cell division protein ZapD -
  WIR04_RS02305 coaE 485265..485879 (-) 615 WP_338890143.1 dephospho-CoA kinase -
  WIR04_RS02310 pilD 485898..486770 (-) 873 WP_338890145.1 A24 family peptidase Machinery gene
  WIR04_RS02315 pilC 486851..488092 (-) 1242 WP_338890147.1 type II secretion system F family protein Machinery gene
  WIR04_RS02320 pilB 488223..489929 (-) 1707 WP_338890149.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WIR04_RS02325 - 489933..490367 (-) 435 WP_338890151.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WIR04_RS02330 nadC 490678..491541 (-) 864 WP_338890153.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WIR04_RS02335 - 491545..492015 (-) 471 WP_307765790.1 retropepsin-like aspartic protease family protein -
  WIR04_RS02340 ampD 492151..492723 (+) 573 WP_338890157.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WIR04_RS02345 pdhR 492999..493763 (+) 765 WP_005329211.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62551.89 Da        Isoelectric Point: 5.6719

>NTDB_id=956760 WIR04_RS02320 WP_338890149.1 488223..489929(-) (pilB) [Aeromonas rivipollensis strain BE1]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQARAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAALGKLMDTEQDTLGMDHIDEAEI
SELEVSDEGSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRSRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNLDQLGFDDRQKAQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEEVPEAELLEIGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEVMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=956760 WIR04_RS02320 WP_338890149.1 488223..489929(-) (pilB) [Aeromonas rivipollensis strain BE1]
ATGACCTCTAGCCTCAATAGCGGCCTGGCAACCAGCCTGGCCGCCTCTTCCCTCCTCAGTGAGCCTGACTCACAGCGCTA
TCTGAGCCAGGCAAGGGCGCAGCGCAAACCCTTTGTCACCTTCCTCATCGAGAACGACATACTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAATTGATTGAGAAGCACCACGTCCTGCCCATCTACACCCAGGGCCACACCCTCTATATTGCCATGTCGGA
CCCGACCAATGTGTCGGCCCTGGAGGATTTCGGCTTCAGCTTCGGCCTGCACACGGAAGCCTTGCTGGTCGAGGAGAGCA
AGCTCACCGCCGCCCTCGGCAAGCTGATGGACACCGAGCAGGACACCCTGGGCATGGATCACATCGACGAGGCCGAGATA
TCGGAGCTCGAGGTGTCCGATGAAGGTTCACGCCTCGACGAGAGCGTCAACACCGCCGACGACGATGCCCCCATAGTCAA
ATACATCCATAAAATCATGATGGATGCCATCAAGCGTGGCGCATCCGACCTGCATTTCGAGCCGTATGAGACCAAGTACA
GGATCCGCTTCCGGATCGACGGCATTCTGCACGAAATCGCCACTCCGCCAGTCAATCTGGCGAACCGTTTCGCGGCCCGT
CTCAAGGTGATGGCGAGGCTCGATATCGCCGAGCGACGCCTGCCCCAGGATGGACGGATCAAGCTGAAGATCTCCCGCAG
CAGATCCATGGACATGCGGGTCAACACCCTGCCCACCATGTGGGGAGAGAAGATCGTCATCCGCTTGCTGGACTCGTCAG
CCGCCCGCCTCAACCTCGATCAGCTCGGCTTCGACGATCGCCAGAAGGCGCAATACCTGCATGCGCTCTCCAAGCCACAG
GGCATGATACTGGTCACGGGCCCCACGGGGTCGGGCAAGACGGTGTCGCTCTATACAGGTCTTAACATCCTCAATACCAG
CGAGGTCAACATCTCCACCGCCGAGGACCCGGTGGAGATCAACTTGCCCGGCGTCAATCAGGTGCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGTTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTGGGCGAGATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAGGCCGCCCAGACCGGTCACCTGGTGCTGTCGACCCTGCATACCAACTCGGCGGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GCCTAGCCCGCAAACTGTGCGATCACTGCAAGGCGCCCGAGGAGGTGCCTGAAGCCGAGTTGCTGGAGATTGGCTTTACC
CAGCAGCAGCTGGCCGCCGGACTGCGGCTGTTCAAGCCGGTCGGCTGCAAAGAGTGTTCGGGAGGATACAGAGGGCGGGT
CGGCATCTATGAAGTCATGCTGATGTCGGACAACATCGCCAAGCTGATCATGCAGGGAGCGAACTCGCTGCAGATCGCGG
CCATCGCCCAGAAGGAGGGCATGCGCACGCTACGCACCTCCGGCCTGGAGAAGGCCCGCCTGGGAGTCACCAGCTTGGCC
GAGATCAACAGGATCACGACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.379

99.824

0.553
  pilB Acinetobacter baylyi ADP1

55.694

98.944

0.551
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

52.364

100

0.526
  pilB Vibrio campbellii strain DS40M4

50.883

99.648

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

49.734

99.12

0.493
  pilF Neisseria gonorrhoeae MS11

46.561

99.824

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

38.448

97.535

0.375
  pilF Thermus thermophilus HB27

39.394

92.958

0.366