Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WJM78_RS12955 Genome accession   NZ_CP149129
Coordinates   2853265..2854404 (+) Length   379 a.a.
NCBI ID   WP_042035531.1    Uniprot ID   -
Organism   Aeromonas popoffii strain BE5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2848265..2859404
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM78_RS12925 - 2848964..2849344 (-) 381 WP_108537062.1 diacylglycerol kinase -
  WJM78_RS12930 - 2849498..2850565 (+) 1068 WP_212513813.1 3-deoxy-7-phosphoheptulonate synthase -
  WJM78_RS12935 - 2850691..2851485 (-) 795 WP_339015854.1 ATP-binding cassette domain-containing protein -
  WJM78_RS12940 btuC 2851472..2852479 (-) 1008 WP_212513812.1 vitamin B12 ABC transporter permease BtuC -
  WJM78_RS12945 - 2852583..2852708 (-) 126 Protein_2518 ABC transporter substrate-binding protein -
  WJM78_RS12950 - 2852850..2853206 (+) 357 WP_042035532.1 DMT family protein -
  WJM78_RS12955 pilU 2853265..2854404 (+) 1140 WP_042035531.1 type IVa pilus ATPase TapW Machinery gene
  WJM78_RS12960 glgC 2854670..2855941 (+) 1272 WP_287147776.1 glucose-1-phosphate adenylyltransferase -
  WJM78_RS12965 - 2856025..2856147 (+) 123 Protein_2522 WbuC family cupin fold metalloprotein -
  WJM78_RS12970 - 2856208..2856339 (-) 132 Protein_2523 IS5/IS1182 family transposase -
  WJM78_RS12975 - 2856610..2858586 (+) 1977 WP_042035529.1 methyl-accepting chemotaxis protein -
  WJM78_RS12980 - 2858775..2859011 (+) 237 WP_042035527.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42175.31 Da        Isoelectric Point: 6.2554

>NTDB_id=956737 WJM78_RS12955 WP_042035531.1 2853265..2854404(+) (pilU) [Aeromonas popoffii strain BE5]
MELRDMLQILAQQDGSDLYLSTGAPPCAKFNGGLRPLLETPLEPGEVANIAAAIMDGEQKLQFEKELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKDKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=956737 WJM78_RS12955 WP_042035531.1 2853265..2854404(+) (pilU) [Aeromonas popoffii strain BE5]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCCAACAGGACGGCTCCGACCTCTATCTGTCAACCGGCGCTCCCCCCTG
CGCCAAGTTCAATGGCGGATTACGTCCCCTTCTGGAGACGCCACTCGAGCCGGGCGAGGTAGCAAACATTGCCGCCGCGA
TCATGGATGGCGAGCAGAAACTGCAATTCGAGAAGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGT
TTCAGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCCCGCAACATCAAAACCGAGATCCCGCGCTTTGA
AGATCTCAAGCTCCCCCCGGTACTGCTCGACACCATCATGGAGAAACGCGGCCTGGTGCTGTTTGTGGGCGGTACCGGCT
CGGGCAAGTCGACATCACTTGCCGCGCTTATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTTCACCGCCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTAGATACCCGCAGCTTCCACGC
CGCGCTCAAGAATACCCTGCGCCAGGCGCCGGACGTCATCCTCATCGGCGAAATTCGCGACCGCGAGACCATGGAGCACG
CGCTGGCCTTCTCCGAAACCGGTCACCTTGCCATCTCTACCTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCGGAGGAGCGCCGCCCGCAGCTGCTCAACGATCTGGGCAACAACCTCAAGGCGTTTGTCTCCCAGCG
GCTGGTCAAAACCACGGACGGCGGCCGCCGGGCGGCGGTGGAGATCATGCTCGGCACCCATACCATCCGCGACATGATCA
AGCGCGGTGAATTTGGCGGTCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTCGAAGGGGTGATAGAAGAGGAAGAGGCGGTGAAAAATGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAAGATAAGGGCCAGATAGCCAGCAGCAGCGATGCAACAGGTTGGAGTCTGGAACCCACCAAGGATG
AGAAAGACGATCTCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.867

98.945

0.533
  pilU Acinetobacter baylyi ADP1

52.113

93.668

0.488
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.593

89.446

0.372
  pilT Legionella pneumophila strain ERS1305867

41.617

88.127

0.367
  pilT Legionella pneumophila strain Lp02

41.617

88.127

0.367
  pilT Pseudomonas aeruginosa PAK

40.407

90.765

0.367
  pilT Pseudomonas stutzeri DSM 10701

39.826

90.765

0.361