Detailed information    

insolico Bioinformatically predicted

Overview

Name   priA   Type   Machinery gene
Locus tag   ACIFYW_RS01100 Genome accession   NZ_CP173026
Coordinates   236191..238395 (+) Length   734 a.a.
NCBI ID   WP_114716698.1    Uniprot ID   -
Organism   Vibrio cholerae strain SPH 24-01     
Function   DNA puliing through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 231191..243395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIFYW_RS01080 (ACIFYW_01080) - 232271..233437 (-) 1167 WP_404272687.1 O-succinylhomoserine (thiol)-lyase -
  ACIFYW_RS01085 (ACIFYW_01085) metJ 233705..234022 (+) 318 WP_000796267.1 met regulon transcriptional regulator MetJ -
  ACIFYW_RS01090 (ACIFYW_01090) - 234133..235410 (-) 1278 WP_001278588.1 malic enzyme-like NAD(P)-binding protein -
  ACIFYW_RS01095 (ACIFYW_01095) rpmE 235718..235936 (-) 219 WP_000643446.1 50S ribosomal protein L31 -
  ACIFYW_RS01100 (ACIFYW_01100) priA 236191..238395 (+) 2205 WP_114716698.1 primosomal protein N' Machinery gene
  ACIFYW_RS01105 (ACIFYW_01105) cytR 238701..239708 (+) 1008 WP_000224452.1 DNA-binding transcriptional regulator CytR Regulator
  ACIFYW_RS01110 (ACIFYW_01110) - 239880..240431 (+) 552 WP_001884022.1 SPOR domain-containing protein -
  ACIFYW_RS01115 (ACIFYW_01115) hslV 240568..241125 (+) 558 WP_001960141.1 ATP-dependent protease subunit HslV -
  ACIFYW_RS01120 (ACIFYW_01120) hslU 241191..242522 (+) 1332 WP_019830204.1 HslU--HslV peptidase ATPase subunit -

Sequence

Protein


Download         Length: 734 a.a.        Molecular weight: 82085.40 Da        Isoelectric Point: 8.4843

>NTDB_id=956737 ACIFYW_RS01100 WP_114716698.1 236191..238395(+) (priA) [Vibrio cholerae strain SPH 24-01]
MRPSIARVCLPVPLDKSFDYLIPAHLFPVLGGRVQVPFGRQTLVGIVQSLTHQSDFPIEQLKSVQAVLDDAPVWPDKLQS
LLHWCSQFYHYPLGETYANALPGALRKGKAAELTSHKEWRLTALGQEQLMQGVKRGAVKQAQVLHLLQHGALSHQTLLDE
EVSSTTLKALVEKGWIECEERKPVARPWPQELEAKVDKPRLNQEQAIAIAAVNSQQGFGCFLLEGVTGSGKTEVYLNLIT
PVLARGEQALVLVPEIGLTPQTINRFRQRFNVPVEVMHSALNDTERLNAWLAARDKVAGIVIGTRSALLTPFAKLGIIIV
DEEHDSSYKQQDSLRYHARDVAVMRAHLENIPIVLGSATPALETLHNALTGKYHHLQLTQRAGNALPTRNHALDVKGLYL
ESGLSAPLIAEMRRHLSAGNQVMLFLNRRGFSPALMCHECGWIAECQRCDAYYTYHQHSNEMRCHHCGSQRPVLQQCKGC
GSTQLVTVGVGTEQLEAQLHTLFPEYRSVRIDRDSTRRKGSLESALTAIRKGEYQILIGTQMLAKGHHFPDVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLHKDYHHFALTALEERKLAQLPPYSFLSLF
RAEANHTAQVEDFLRQVRETLRCNPWFDSECMVLGPTPAPLAKRAGKFRWQLLLQTPNRTLMQKILQSARPALQQLPLAS
KVRWSIDIDPQDLS

Nucleotide


Download         Length: 2205 bp        

>NTDB_id=956737 ACIFYW_RS01100 WP_114716698.1 236191..238395(+) (priA) [Vibrio cholerae strain SPH 24-01]
ATGCGACCCTCCATTGCCCGAGTTTGCCTACCTGTACCTCTGGATAAGTCTTTCGACTATCTGATCCCAGCTCATCTATT
TCCAGTGCTGGGCGGTCGTGTGCAAGTGCCTTTTGGGCGACAAACCTTGGTCGGTATTGTCCAGTCGCTGACACATCAAT
CCGATTTTCCTATCGAACAACTGAAATCCGTGCAAGCCGTTTTGGATGACGCGCCCGTGTGGCCAGATAAACTGCAGTCT
CTGCTGCACTGGTGTAGTCAGTTTTATCATTATCCGCTCGGAGAGACGTACGCGAATGCCTTGCCCGGCGCACTGCGCAA
AGGCAAGGCGGCAGAGTTGACTAGCCATAAGGAGTGGCGTTTGACCGCGCTTGGTCAAGAGCAACTGATGCAAGGGGTGA
AGCGTGGCGCAGTAAAGCAAGCGCAAGTCCTTCACCTGCTGCAGCACGGTGCTTTATCGCATCAAACTTTGCTTGATGAA
GAGGTGAGCAGCACGACCCTCAAAGCCTTGGTCGAAAAAGGGTGGATTGAGTGTGAAGAGCGTAAACCTGTGGCGCGTCC
TTGGCCGCAAGAGCTGGAAGCTAAAGTCGATAAACCTAGGCTTAATCAAGAGCAGGCGATCGCGATTGCGGCGGTGAATA
GCCAGCAAGGTTTCGGCTGCTTTTTATTAGAAGGGGTAACGGGCTCAGGCAAAACAGAGGTGTATCTTAACCTCATCACA
CCGGTATTGGCGCGTGGTGAACAAGCTTTGGTGTTGGTGCCGGAAATTGGTTTAACCCCACAAACCATCAACCGGTTTCG
TCAACGTTTTAATGTCCCCGTCGAAGTCATGCACTCGGCGCTCAACGACACTGAACGCCTCAATGCTTGGCTGGCGGCGC
GCGATAAAGTGGCGGGGATTGTGATTGGTACGCGCTCGGCGCTGCTCACACCCTTTGCCAAATTGGGCATTATTATTGTT
GATGAAGAACATGATAGTTCTTACAAGCAGCAAGATAGTCTGCGTTATCACGCCCGAGATGTCGCTGTGATGCGCGCGCA
TTTGGAAAACATTCCGATCGTGCTTGGCTCGGCGACGCCAGCCTTGGAAACGTTGCATAATGCGCTCACTGGTAAATACC
ATCACTTGCAACTGACGCAGCGTGCTGGTAATGCACTACCCACACGTAACCACGCGTTGGATGTGAAAGGGCTCTATCTG
GAAAGTGGCCTGTCTGCGCCTTTGATTGCGGAAATGCGCCGCCATTTGTCTGCGGGCAATCAGGTGATGCTGTTTTTAAA
CCGCCGCGGTTTTTCTCCGGCTTTGATGTGCCATGAATGCGGCTGGATTGCGGAGTGCCAGCGCTGTGATGCCTATTACA
CCTACCATCAACACAGCAATGAAATGCGCTGCCACCACTGTGGTTCACAGCGTCCTGTTCTCCAGCAATGCAAAGGGTGC
GGCTCGACTCAACTGGTTACTGTCGGCGTTGGTACGGAACAGCTTGAAGCGCAATTACACACTCTATTCCCTGAGTATCG
TTCAGTGCGGATTGATCGAGATAGCACGCGGCGTAAAGGCAGTTTGGAATCGGCGTTAACCGCGATTCGCAAAGGCGAAT
ATCAGATTTTGATTGGTACGCAAATGCTCGCCAAAGGACATCACTTCCCCGATGTTACCTTGGTCGCGTTGTTGGATGTC
GATGGCTCATTGTACAGCAGTGATTTTCGCGCTTCTGAGCGTTTGGCTCAGCTCTTTATTCAAGTCGCAGGGCGGGCAGG
GCGTGCCAGCAAGCCGGGCGAAGTGGTGCTACAAACGCATCATCCCGAACACAGTTTGCTACAAGCCTTGCTGCATAAGG
ATTACCACCATTTTGCCCTGACGGCGTTGGAAGAGCGCAAGCTGGCGCAGTTGCCCCCTTATTCTTTCTTGAGCTTATTT
CGAGCCGAAGCCAATCACACCGCTCAAGTGGAAGATTTTTTGCGTCAAGTGCGCGAAACCTTGCGCTGCAATCCTTGGTT
TGATAGCGAATGTATGGTGCTCGGGCCAACGCCTGCGCCACTCGCGAAACGAGCGGGAAAATTCCGTTGGCAACTTTTAC
TACAAACGCCGAATCGCACCCTAATGCAGAAGATTTTGCAAAGCGCACGTCCAGCACTGCAACAATTACCGCTGGCGAGC
AAGGTGCGCTGGTCGATCGATATTGATCCGCAAGATTTGAGCTAA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(204-364)

3DNA-binding domain (3BD) (PriA_3primeBD)

(7-102)

Primosomal protein N C-terminal domain (PriA_C)

(633-730)

PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA)

(446-472)

Helicase conserved C-terminal domain (Helicase_C)

(515-589)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  priA Acinetobacter baumannii D1279779

43.708

100

0.44