Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WJM57_RS18295 Genome accession   NZ_CP149127
Coordinates   3948906..3950015 (+) Length   369 a.a.
NCBI ID   WP_339021807.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain BE4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3943906..3955015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM57_RS18260 - 3944495..3944794 (+) 300 WP_011899099.1 hypothetical protein -
  WJM57_RS18265 - 3944863..3945282 (-) 420 WP_005320482.1 DUF4426 domain-containing protein -
  WJM57_RS18270 yggU 3945301..3945600 (-) 300 WP_005320479.1 DUF167 family protein YggU -
  WJM57_RS18275 - 3945600..3946151 (-) 552 WP_005320477.1 YggT family protein -
  WJM57_RS18280 proC 3946180..3947004 (-) 825 WP_339021806.1 pyrroline-5-carboxylate reductase -
  WJM57_RS18285 - 3947095..3947796 (-) 702 WP_270688184.1 YggS family pyridoxal phosphate-dependent enzyme -
  WJM57_RS18290 pilT 3947836..3948870 (+) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  WJM57_RS18295 pilU 3948906..3950015 (+) 1110 WP_339021807.1 type IVa pilus ATPase TapU Machinery gene
  WJM57_RS18300 yaaA 3950047..3950820 (+) 774 WP_059169418.1 peroxide stress protein YaaA -
  WJM57_RS18305 srmB 3951009..3952232 (-) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  WJM57_RS18310 - 3952360..3953070 (+) 711 WP_339021808.1 methyltransferase -
  WJM57_RS18315 brnQ 3953256..3954569 (+) 1314 WP_017412062.1 branched-chain amino acid transport system II carrier protein -
  WJM57_RS18320 - 3954645..3954875 (-) 231 WP_005320453.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41073.12 Da        Isoelectric Point: 6.3717

>NTDB_id=956722 WJM57_RS18295 WP_339021807.1 3948906..3950015(+) (pilU) [Aeromonas salmonicida strain BE4]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLFSLGSEALDKQSALTLVRETLSPEHFERYIRTKEGNYAIHREALGR
FRVSAFWQQELPAMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=956722 WJM57_RS18295 WP_339021807.1 3948906..3950015(+) (pilU) [Aeromonas salmonicida strain BE4]
ATGAATCTGGATGCCCTGTTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGTACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGTTCTCCCTCGGCAGCGAAGCGCTCGACAAGCAGAGCGCGCTGACCCTGGTCAGGGAAA
CGCTCAGCCCTGAGCACTTCGAACGTTACATCCGCACCAAGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTAGGGTGAGTGCCTTCTGGCAGCAGGAGCTGCCGGCCATGGTGGTGCGCCGTATCGAGACCCGCATTCCAACCTTTGA
CGAACTGACCCTGCCGCCCATCTTGCAGGAGGTGGCCATGGCCAAACGCGGCTTGGTGCTGTTCGTCGGTGCCACCGGAA
CGGGCAAATCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGCATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCATCTTTGCCTCGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAACACCGCCAGTTACTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCGGCCTTTGAAGTGCTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAGTCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.967

99.729

0.588
  pilU Pseudomonas stutzeri DSM 10701

58.939

97.019

0.572
  pilU Acinetobacter baylyi ADP1

57.507

95.664

0.55
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.692

89.702

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.419

90.515

0.366
  pilT Vibrio cholerae strain A1552

40.419

90.515

0.366