Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WJM28_RS18620 Genome accession   NZ_CP149125
Coordinates   4032456..4034162 (+) Length   568 a.a.
NCBI ID   WP_041213997.1    Uniprot ID   A0A2X4NBG7
Organism   Aeromonas caviae strain BE3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4027456..4039162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM28_RS18595 pdhR 4028622..4029386 (-) 765 WP_010675815.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WJM28_RS18600 ampD 4029663..4030235 (-) 573 WP_109421774.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WJM28_RS18605 - 4030364..4030834 (+) 471 WP_042015101.1 retropepsin-like aspartic protease -
  WJM28_RS18610 nadC 4030838..4031701 (+) 864 WP_042864860.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WJM28_RS18615 - 4032012..4032179 (+) 168 Protein_3584 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WJM28_RS18620 pilB 4032456..4034162 (+) 1707 WP_041213997.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WJM28_RS18625 pilC 4034290..4035531 (+) 1242 WP_339331829.1 type II secretion system F family protein Machinery gene
  WJM28_RS18630 pilD 4035609..4036484 (+) 876 WP_339331830.1 A24 family peptidase Machinery gene
  WJM28_RS18635 coaE 4036503..4037117 (+) 615 WP_048208563.1 dephospho-CoA kinase -
  WJM28_RS18640 zapD 4037155..4037877 (+) 723 WP_010675825.1 cell division protein ZapD -
  WJM28_RS18645 yacG 4037888..4038082 (+) 195 WP_039040499.1 DNA gyrase inhibitor YacG -
  WJM28_RS18650 mutT 4038140..4038544 (-) 405 WP_171864957.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62566.95 Da        Isoelectric Point: 5.8214

>NTDB_id=956692 WJM28_RS18620 WP_041213997.1 4032456..4034162(+) (pilB) [Aeromonas caviae strain BE3]
MTSSLNSGLATSLAASSLLSEPDSQRYLSQAKAQRKPFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEESKLTAAIGKLMESEQDTLGMDHIDEAEI
SELEVSDESSRLDESVNTADDDAPIVKYIHKIMMDAIKRGASDLHFEPYETKYRIRFRVDGILHEVATPPVNLANRFAAR
LKVMARLDIAERRLPQDGRIKLKISRNRSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIDQLGFDDRQKTQYLHALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTSEVNISTAEDPVEINLPGVNQVQINPKAGLTFASALRSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYRGRVGIYEIMLMSDNIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRITTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=956692 WJM28_RS18620 WP_041213997.1 4032456..4034162(+) (pilB) [Aeromonas caviae strain BE3]
ATGACCTCTAGCCTTAATAGCGGCCTGGCCACCAGCTTGGCCGCTTCTTCCCTCCTCAGTGAGCCGGACTCACAGCGCTA
TCTGAGCCAGGCCAAGGCGCAGCGCAAGCCCTTTGTCACCTTCCTGATTGAAAACGACATCCTCGACAGCAAAGCCCTCG
CCGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTGGCGGCCTTTGATCTCGCCGAGATCCCGCAGAAATAC
CTCAATCAAAAGCTGATTGAAAAGCACCATGTCCTGCCCATTTACACCCAGGGGCATACCCTCTATATCGCCATGTCCGA
TCCGACCAATGTGTCGGCTCTGGAGGATTTTGGCTTCAGCTTCGGCCTGCATACGGAAGCCCTGCTGGTCGAAGAGAGCA
AGCTGACCGCCGCCATCGGCAAGCTGATGGAGAGCGAGCAGGATACCCTGGGCATGGATCATATCGACGAGGCCGAGATC
TCCGAACTCGAGGTTTCCGACGAGAGTTCCCGCCTCGACGAGAGCGTCAACACCGCGGACGATGACGCCCCTATCGTCAA
ATACATCCACAAGATCATGATGGATGCCATCAAGCGCGGGGCCTCCGACCTGCATTTCGAACCCTACGAGACCAAGTACC
GGATCCGCTTTCGGGTAGACGGCATACTGCACGAAGTCGCCACGCCGCCGGTCAACCTGGCCAACCGCTTCGCAGCTCGC
CTCAAGGTGATGGCACGGCTCGACATTGCCGAACGGCGCCTGCCCCAGGATGGCCGGATCAAGCTCAAGATCTCACGCAA
CAGATCCATGGACATGCGTGTCAACACCCTGCCCACCATGTGGGGCGAAAAGATCGTGATCCGGTTGCTGGACTCCTCGG
CCGCCCGGCTCAATATCGATCAGCTCGGCTTTGACGACCGGCAGAAGACGCAATACCTGCACGCCCTCTCCAAACCTCAG
GGCATGATCCTGGTGACCGGCCCGACGGGGTCAGGCAAGACGGTCTCACTCTATACCGGCCTCAACATCCTCAACACCAG
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTCGAGATCAACCTGCCCGGCGTCAACCAGGTTCAGATCAATCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCCTGCGCCAGGATCCCGACATCGTCATGGTAGGGGAGATACGGGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGTCACCTCGTGCTCTCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTTACCCGGATGATGAACATGGGGGTGCCGGCCTTCAACATCGCCTCTTCGGTCACCCTCATCATGGCCCAGC
GCCTTGCCCGCAAACTCTGCGATCACTGCAAGGCGCCGGAAGTGGTGCCGGAAGCCGAGTTGCTGGAGCTGGGCTTCACC
CAGCAGCAACTGGCCGCCGGGTTGCGGCTGTTCAAACCCGTGGGATGCAAGGAGTGCTCGGGAGGCTACAGAGGCCGGGT
CGGCATTTATGAGATCATGCTGATGTCCGACAACATCGCCAAACTCATCATGCAGGGAGCCAACTCGCTGCAGATTGCCG
CGATAGCCCAAAAAGAAGGGATGCGCACCCTGCGTACATCCGGCCTCGAGAAAGCGCGTCTTGGCGTCACCAGCCTGGCC
GAAATCAACAGGATCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X4NBG7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.026

99.824

0.549
  pilB Acinetobacter baylyi ADP1

55.062

99.12

0.546
  pilB Legionella pneumophila strain ERS1305867

54.867

99.472

0.546
  pilB Vibrio cholerae strain A1552

53.415

100

0.537
  pilB Vibrio campbellii strain DS40M4

50.53

99.648

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

50.089

99.12

0.496
  pilF Neisseria gonorrhoeae MS11

46.561

99.824

0.465
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

37.975

97.359

0.37