Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WJL51_RS17525 Genome accession   NZ_CP149124
Coordinates   3873618..3875333 (-) Length   571 a.a.
NCBI ID   WP_200871048.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain BE2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3868618..3880333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJL51_RS17495 mutT 3869294..3869707 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  WJL51_RS17500 yacG 3869768..3869962 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  WJL51_RS17505 zapD 3869972..3870694 (-) 723 WP_024945431.1 cell division protein ZapD -
  WJL51_RS17510 coaE 3870732..3871346 (-) 615 WP_024945430.1 dephospho-CoA kinase -
  WJL51_RS17515 pilD 3871366..3872238 (-) 873 WP_339330058.1 A24 family peptidase Machinery gene
  WJL51_RS17520 pilC 3872261..3873502 (-) 1242 WP_043126919.1 type II secretion system F family protein Machinery gene
  WJL51_RS17525 pilB 3873618..3875333 (-) 1716 WP_200871048.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WJL51_RS17530 - 3875324..3875764 (-) 441 WP_339330059.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WJL51_RS17535 nadC 3876087..3876944 (-) 858 WP_339330060.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WJL51_RS17540 - 3876949..3877419 (-) 471 WP_024944772.1 retropepsin-like aspartic protease -
  WJL51_RS17545 ampD 3877567..3878139 (+) 573 WP_339330061.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WJL51_RS17550 pdhR 3878415..3879179 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62910.42 Da        Isoelectric Point: 5.4342

>NTDB_id=956665 WJL51_RS17525 WP_200871048.1 3873618..3875333(-) (pilB) [Aeromonas hydrophila strain BE2]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMENDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTTDDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=956665 WJL51_RS17525 WP_200871048.1 3873618..3875333(-) (pilB) [Aeromonas hydrophila strain BE2]
CTGCCCATAATGACATCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTC
ACAACGCTATCTGAGCCAAGCCAAGGCGCAGCGCAAACCCTTTGTGACTTTTCTGATCGAAAACGAAATACTCGACAGCA
AGGCACTGGCTGACTTCTGCGAGCTGGAGTATGGAGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAATACCTCAATCAGAAGCTGATTGAAAAACATCATGTCTTGCCCATCTACACTCAGGGACACACCCTCTATATCGC
CATGTCGGATCCCACCAATGTCTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTAG
AAGAGAACAAGCTCACCACAGCTATCGGCAAGCTGATGGAGAACGATCAGGATGCCCTCGGCATGGAGGACATCGACGAG
TCCGAGATCTCCGAACTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAAAGCGTCAATACCACCGACGATGACGCGCC
CATCGTCAAATACATCAACAAGATCATGATGGATGCCATCAAGCGCGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGA
CCAAATACCGCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCGACTCCACCTGTCAATCTGGCCAACCGCTTC
TCGGCCCGCCTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCACAGGATGGTCGCATCAAACTGAAGCT
GTCGCGCAACAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCG
ACTCCTCCGCGGCACGCCTCAATATCGAGCAGCTCGGGTTTGACGATCGCCAGAAGGCACAATATCTGCGTGCCCTCTCG
AAGCCTCAGGGCATGATTCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCT
CAATACCACCGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAGGTCA
ATCCCAAGGCAGGACTGACCTTCGCGAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCGGATGTGGTCATGGTAGGTGAA
ATCCGGGATCTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACTTGGTGCTATCCACCCTGCACACCAA
CTCGGCGGCAGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCA
TGGCTCAGCGGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTG
GGTTTTACCCAGCAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGTTCAGGTGGGTACAA
GGGCCGGGTCGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAAGGGGCCAACTCTCTGC
AAATTGCCGCCATTGCCCAGAAGGAAGGGATGCGTACCTTGCGCACCTCCGGCCTTGAGAAGGCCCGCCTGGGGGTGACC
AGTCTGGCGGAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

100

0.557
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.24

99.124

0.538
  pilB Vibrio cholerae strain A1552

53.982

98.949

0.534
  pilB Vibrio campbellii strain DS40M4

51.943

99.124

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.424

0.506
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

96.848

0.377
  pilF Thermus thermophilus HB27

40.152

92.469

0.371