Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WD047_RS02430 Genome accession   NZ_CP148749
Coordinates   518320..520026 (-) Length   568 a.a.
NCBI ID   WP_139748842.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AH230115     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 513320..525026
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WD047_RS02400 mutT 513997..514410 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  WD047_RS02405 yacG 514471..514665 (-) 195 WP_139748838.1 DNA gyrase inhibitor YacG -
  WD047_RS02410 zapD 514675..515397 (-) 723 WP_024945431.1 cell division protein ZapD -
  WD047_RS02415 coaE 515435..516049 (-) 615 WP_219305925.1 dephospho-CoA kinase -
  WD047_RS02420 pilD 516068..516940 (-) 873 WP_139748840.1 A24 family peptidase Machinery gene
  WD047_RS02425 pilC 516963..518204 (-) 1242 WP_139748841.1 type II secretion system F family protein Machinery gene
  WD047_RS02430 pilB 518320..520026 (-) 1707 WP_139748842.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WD047_RS02435 - 520031..520495 (-) 465 WP_139748843.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WD047_RS02440 nadC 520818..521675 (-) 858 WP_139748844.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WD047_RS02445 - 521681..522151 (-) 471 WP_024944772.1 retropepsin-like aspartic protease -
  WD047_RS02450 ampD 522298..522870 (+) 573 WP_139748845.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WD047_RS02455 pdhR 523146..523910 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62598.98 Da        Isoelectric Point: 5.4342

>NTDB_id=955418 WD047_RS02430 WP_139748842.1 518320..520026(-) (pilB) [Aeromonas hydrophila strain AH230115]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMENDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTTDDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKTQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=955418 WD047_RS02430 WP_139748842.1 518320..520026(-) (pilB) [Aeromonas hydrophila strain AH230115]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCTTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCTCAGCGTAAACCCTTTGTGACCTTTCTGATCGAAAATGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAT
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCCACCAATGTATCTGCACTGGAGGATTTTGGTTTCAGCTTCGGGTTGCATACCGAAGCGCTGCTGGTAGAGGAGAACA
AACTCACCACAGCTATCGGCAAGCTGATGGAGAACGATCAGGATGCCCTCGGCATGGAGGACATCGACGAGTCCGAGATC
TCCGAGCTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAAAGCGTCAATACCACCGACGATGACGCTCCAATTGTAAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGAATAGACGGTATTCTGCATGAGATAGCGACCCCACCGGTCAACCTAGCCAACCGCTTCTCGGCTCGC
CTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTCAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTTTGCTCGACTCCTCCG
CCGCACGCCTCAATATCGAGCAGCTCGGTTTCGATGATCGGCAAAAAACGCAGTATCTGCGTGCTCTGTCGAAGCCTCAG
GGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATTTCCACCGCCGAGGATCCGGTGGAAATCAACCTGCCTGGCGTCAATCAGGTGCAGGTCAATCCCAAGG
CAGGACTGACATTCGCCAGCGCCTTGCGCTCTTTCTTGCGACAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAAACAGCCGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCGGC
GGAAACGCTGACCCGGATGATGAACATGGGGGTACCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCGGGTGGATACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAATATCGCAAAATTGATCATGCAGGGGGCTAACTCTCTGCAAATTGCCG
CCATCGCTCAGAAGGAAGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGTCTGGGAGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.536

100

0.556
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.417

99.648

0.542
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373