Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   WD047_RS02420 Genome accession   NZ_CP148749
Coordinates   516068..516940 (-) Length   290 a.a.
NCBI ID   WP_139748840.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain AH230115     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 511068..521940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WD047_RS02395 - 512804..513988 (+) 1185 WP_338883560.1 tetratricopeptide repeat protein -
  WD047_RS02400 mutT 513997..514410 (+) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -
  WD047_RS02405 yacG 514471..514665 (-) 195 WP_139748838.1 DNA gyrase inhibitor YacG -
  WD047_RS02410 zapD 514675..515397 (-) 723 WP_024945431.1 cell division protein ZapD -
  WD047_RS02415 coaE 515435..516049 (-) 615 WP_219305925.1 dephospho-CoA kinase -
  WD047_RS02420 pilD 516068..516940 (-) 873 WP_139748840.1 A24 family peptidase Machinery gene
  WD047_RS02425 pilC 516963..518204 (-) 1242 WP_139748841.1 type II secretion system F family protein Machinery gene
  WD047_RS02430 pilB 518320..520026 (-) 1707 WP_139748842.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  WD047_RS02435 - 520031..520495 (-) 465 WP_139748843.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  WD047_RS02440 nadC 520818..521675 (-) 858 WP_139748844.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32463.85 Da        Isoelectric Point: 6.7410

>NTDB_id=955416 WD047_RS02420 WP_139748840.1 516068..516940(-) (pilD) [Aeromonas hydrophila strain AH230115]
MLLITDVFHSLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYLGYFNPEALPQQEERYNLMVPRSACPHCGHA
ITAMENIPLLSWLWLKGRCRECQAPISARYPLVELLTALLSLVVATTFPPGWALLAALLLTWVLVALTFIDLDKMLLPDQ
LTLPLLWGGLLFNLAGGFAPLADAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL
VGALMGIGLILLRNHHQNKPIPFGPYLAIAGWIALLWGDTITRWYLTTFL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=955416 WD047_RS02420 WP_139748840.1 516068..516940(-) (pilD) [Aeromonas hydrophila strain AH230115]
ATGTTGCTGATCACTGACGTTTTTCACAGCCTGCCATGGCTCTATTTCTCGCTGGTATTCCTCTTTTCCCTGATGATCGG
CAGTTTTCTCAACGTGGTCATCCATCGCCTGCCCATCATGCTGGAGCGAGAGTGGCAGGCCGAATATCTCGGCTATTTCA
ATCCGGAAGCACTGCCGCAGCAGGAAGAGCGCTACAACCTGATGGTGCCGCGTTCTGCCTGTCCTCATTGCGGCCACGCC
ATCACGGCCATGGAGAACATCCCCCTGCTCAGCTGGCTCTGGCTCAAGGGACGCTGCCGTGAATGCCAGGCACCCATATC
GGCCCGTTATCCTCTGGTGGAGCTGCTGACGGCCCTGCTCTCGCTGGTCGTCGCCACAACATTCCCACCGGGCTGGGCTT
TGCTGGCCGCCCTGCTGCTCACCTGGGTACTGGTGGCACTCACTTTCATCGATCTGGACAAGATGCTGCTGCCGGATCAG
CTGACACTGCCACTGCTGTGGGGCGGCCTGCTGTTCAATTTGGCTGGGGGATTTGCACCGCTGGCCGATGCGGTGATCGG
CGCCATGGCCGGCTATCTGGTGCTCTGGAGCCTCTACTGGGCCTTCAAGTTGCTGACCGGCAAGGAAGGGATGGGCTATG
GTGACTTCAAGCTGCTGGCGGCGCTGGGTGCCTGGCTGGGCTGGCAGGCACTGCCCATCGTACTGCTGCTCTCCTCCCTA
GTTGGCGCCCTCATGGGCATAGGCCTGATCCTGCTGCGCAACCATCATCAGAACAAACCCATCCCCTTCGGCCCCTATCT
GGCCATCGCCGGCTGGATCGCGCTGCTGTGGGGCGATACCATCACCCGCTGGTACCTCACGACGTTTCTCTGA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(139-247)

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

62.021

98.966

0.614
  pilD Vibrio campbellii strain DS40M4

55.944

98.621

0.552
  pilD Acinetobacter baumannii D1279779

53.873

97.931

0.528
  pilD Acinetobacter nosocomialis M2

53.873

97.931

0.528
  pilD Neisseria gonorrhoeae MS11

48.718

94.138

0.459