Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ACFELB_RS24375 Genome accession   NZ_CP172850
Coordinates   5241058..5241525 (-) Length   155 a.a.
NCBI ID   WP_074227024.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238638E_ID017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5236058..5246525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELB_RS24355 (ACFELB_24355) nadC 5237236..5238084 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELB_RS24370 (ACFELB_24370) - 5239590..5240984 (-) 1395 WP_400589685.1 O-antigen ligase family protein -
  ACFELB_RS24375 (ACFELB_24375) pilA/pilAI 5241058..5241525 (-) 468 WP_074227024.1 pilin Machinery gene
  ACFELB_RS24380 (ACFELB_24380) pilB 5241756..5243456 (+) 1701 WP_058355294.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELB_RS24385 (ACFELB_24385) pilC 5243460..5244677 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  ACFELB_RS24390 (ACFELB_24390) pilD 5244678..5245550 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELB_RS24395 (ACFELB_24395) coaE 5245547..5246158 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  ACFELB_RS24400 (ACFELB_24400) yacG 5246155..5246355 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16277.67 Da        Isoelectric Point: 8.4578

>NTDB_id=954749 ACFELB_RS24375 WP_074227024.1 5241058..5241525(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 238638E_ID017]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSTKTPNNTQYDIGFTE
STLLDGTGKGQITVTDNKDGTVQLVATLGGKAAGSAIKGAIITVSRDDEGVWSCKITKTPTAWKANYAPANCPKS

Nucleotide


Download         Length: 468 bp        

>NTDB_id=954749 ACFELB_RS24375 WP_074227024.1 5241058..5241525(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 238638E_ID017]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCTGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCACCAAGACCCCTAACAATACCCAGTATGACATTGGCTTTACTGAA
TCTACTTTGCTGGATGGTACTGGTAAGGGCCAGATCACTGTAACGGACAATAAAGATGGTACCGTGCAACTGGTCGCTAC
TCTGGGGGGTAAGGCTGCTGGTTCCGCTATCAAAGGTGCAATTATTACTGTATCGCGTGACGATGAAGGTGTGTGGAGCT
GCAAAATCACCAAGACTCCCACGGCTTGGAAAGCCAACTACGCTCCCGCCAACTGCCCGAAATCTTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-152)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.156

99.355

0.439
  pilA Ralstonia pseudosolanacearum GMI1000

40

100

0.439
  pilA Acinetobacter baumannii strain A118

44.079

98.065

0.432
  pilA Pseudomonas aeruginosa PAK

47.445

88.387

0.419
  pilA2 Legionella pneumophila strain ERS1305867

37.037

100

0.387
  comP Acinetobacter baylyi ADP1

36.585

100

0.387
  pilA/pilAII Pseudomonas stutzeri DSM 10701

37.419

100

0.374
  pilA2 Legionella pneumophila str. Paris

35.849

100

0.368