Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ACFELG_RS23505 Genome accession   NZ_CP172848
Coordinates   5027479..5027946 (-) Length   155 a.a.
NCBI ID   WP_400581626.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 238628E_ID004     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5022479..5032946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFELG_RS23485 (ACFELG_23485) - 5022566..5024821 (-) 2256 WP_003104913.1 DUF1631 domain-containing protein -
  ACFELG_RS23490 (ACFELG_23490) nadC 5025107..5025955 (+) 849 WP_016562338.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACFELG_RS23500 (ACFELG_23500) - 5026138..5027313 (-) 1176 WP_400581624.1 O-antigen ligase family protein -
  ACFELG_RS23505 (ACFELG_23505) pilA/pilAI 5027479..5027946 (-) 468 WP_400581626.1 pilin Machinery gene
  ACFELG_RS23510 (ACFELG_23510) pilB 5028177..5029877 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFELG_RS23515 (ACFELG_23515) pilC 5029881..5031098 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  ACFELG_RS23520 (ACFELG_23520) pilD 5031099..5031971 (+) 873 WP_003107301.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  ACFELG_RS23525 (ACFELG_23525) coaE 5031968..5032579 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  ACFELG_RS23530 (ACFELG_23530) yacG 5032576..5032776 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16334.72 Da        Isoelectric Point: 8.9987

>NTDB_id=954544 ACFELG_RS23505 WP_400581626.1 5027479..5027946(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 238628E_ID004]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSATPKDTQYDIGFTE
STLLDGSGKSQIQVTDNKDGTVELVATLGGKSSGSAIKGAVITVSRKNDGVWNCKITKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 468 bp        

>NTDB_id=954544 ACFELG_RS23505 WP_400581626.1 5027479..5027946(-) (pilA/pilAI) [Pseudomonas aeruginosa strain 238628E_ID004]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCGCGACTCCTAAAGATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCCAGGTAACGGACAATAAAGATGGCACCGTTGAGTTGGTCGCTAC
CTTGGGGGGTAAATCTTCTGGTTCCGCTATCAAAGGGGCTGTAATCACTGTTTCGCGTAAAAATGACGGAGTCTGGAACT
GCAAAATCACCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-152)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.156

99.355

0.439
  pilA Acinetobacter baumannii strain A118

44.444

98.71

0.439
  pilA2 Legionella pneumophila strain ERS1305867

40.26

99.355

0.4
  pilA Pseudomonas aeruginosa PAK

44.853

87.742

0.394
  comP Acinetobacter baylyi ADP1

37.195

100

0.394
  pilA2 Legionella pneumophila str. Paris

39.61

99.355

0.394
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.065

100

0.381
  pilA Vibrio parahaemolyticus RIMD 2210633

37.013

99.355

0.368