Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACHP5R_RS20495 Genome accession   NZ_CP172134
Coordinates   4203872..4205305 (+) Length   477 a.a.
NCBI ID   WP_000809051.1    Uniprot ID   A0A0H2VDV8
Organism   Escherichia coli strain GF131     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4198872..4210305
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHP5R_RS20465 (ACHP5R_20465) yrbG 4199430..4200407 (+) 978 WP_089583335.1 calcium/sodium antiporter -
  ACHP5R_RS20470 (ACHP5R_20470) kdsD 4200421..4201407 (+) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  ACHP5R_RS20475 (ACHP5R_20475) kdsC 4201428..4201994 (+) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACHP5R_RS20480 (ACHP5R_20480) lptC 4201991..4202566 (+) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  ACHP5R_RS20485 (ACHP5R_20485) lptA 4202535..4203092 (+) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACHP5R_RS20490 (ACHP5R_20490) lptB 4203099..4203824 (+) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  ACHP5R_RS20495 (ACHP5R_20495) rpoN/rpoN1 4203872..4205305 (+) 1434 WP_000809051.1 RNA polymerase factor sigma-54 Machinery gene
  ACHP5R_RS20500 (ACHP5R_20500) hpf 4205328..4205615 (+) 288 WP_001176599.1 ribosome hibernation promoting factor -
  ACHP5R_RS20505 (ACHP5R_20505) ptsN 4205733..4206224 (+) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACHP5R_RS20510 (ACHP5R_20510) rapZ 4206270..4207124 (+) 855 WP_000243741.1 RNase adapter RapZ -
  ACHP5R_RS20515 (ACHP5R_20515) npr 4207121..4207393 (+) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  ACHP5R_RS20520 (ACHP5R_20520) yrbL 4207606..4208238 (+) 633 WP_000620399.1 PhoP regulatory network protein YrbL -
  ACHP5R_RS20525 (ACHP5R_20525) mtgA 4208235..4208963 (-) 729 WP_000047098.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACHP5R_RS20530 (ACHP5R_20530) elbB 4208960..4209613 (-) 654 WP_001302019.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53993.79 Da        Isoelectric Point: 4.3639

>NTDB_id=950266 ACHP5R_RS20495 WP_000809051.1 4203872..4205305(+) (rpoN/rpoN1) [Escherichia coli strain GF131]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=950266 ACHP5R_RS20495 WP_000809051.1 4203872..4205305(+) (rpoN/rpoN1) [Escherichia coli strain GF131]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAACTGCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACACTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
CGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGTTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACTGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATTGACATCGACGAAGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTGGCGGCAAAAGATCTGCGTGACTGCCTGCTGATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATTATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCGGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAAGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2VDV8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.223

100

0.465