Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WDM00_RS02540 Genome accession   NZ_CP147726
Coordinates   525420..525842 (-) Length   140 a.a.
NCBI ID   WP_148553901.1    Uniprot ID   -
Organism   Vibrio cholerae strain CNCTC 6536     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 520420..530842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDM00_RS02520 (WDM00_02520) coaE 520918..521526 (-) 609 WP_337227815.1 dephospho-CoA kinase -
  WDM00_RS02525 (WDM00_02525) pilD 521523..522398 (-) 876 WP_000418747.1 A24 family peptidase Machinery gene
  WDM00_RS02530 (WDM00_02530) pilC 522456..523682 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  WDM00_RS02535 (WDM00_02535) pilB 523725..525413 (-) 1689 WP_367007827.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WDM00_RS02540 (WDM00_02540) pilA 525420..525842 (-) 423 WP_148553901.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  WDM00_RS02545 (WDM00_02545) nadC 526087..526977 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WDM00_RS02550 (WDM00_02550) ampD 527279..527824 (+) 546 WP_148553902.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WDM00_RS02555 (WDM00_02555) fldB 528006..528524 (-) 519 WP_019828645.1 flavodoxin FldB -
  WDM00_RS02560 (WDM00_02560) xerD 528662..529570 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  WDM00_RS02565 (WDM00_02565) dsbC 529587..530339 (+) 753 WP_000115049.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14739.09 Da        Isoelectric Point: 9.4528

>NTDB_id=948227 WDM00_RS02540 WP_148553901.1 525420..525842(-) (pilA) [Vibrio cholerae strain CNCTC 6536]
MKAYKNKQQQGFTLIELMIVVAIIGVLAAIAVPAYKDYVTKGEAAAALSTLKALQTPAELLYQDKGAFVATDLTSLGVSS
ASSQLGTLAIDTAKSAAVKFTFTTPSLVNGVMTLERTTDGWTCTRSANSKLTSLDIKGCR

Nucleotide


Download         Length: 423 bp        

>NTDB_id=948227 WDM00_RS02540 WP_148553901.1 525420..525842(-) (pilA) [Vibrio cholerae strain CNCTC 6536]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTATTGGTGTGTT
GGCAGCGATTGCCGTGCCTGCTTATAAGGATTATGTCACTAAGGGAGAAGCTGCTGCTGCATTATCAACACTGAAAGCTT
TGCAGACCCCTGCTGAACTCCTATATCAGGATAAAGGTGCTTTTGTAGCAACTGATCTAACGAGTCTAGGAGTATCAAGT
GCTAGTAGCCAGCTAGGTACTTTAGCAATAGATACCGCTAAGTCTGCAGCCGTAAAATTCACATTTACTACACCTTCTTT
AGTCAATGGAGTCATGACGCTAGAAAGAACTACAGATGGCTGGACTTGTACGAGAAGTGCAAATAGCAAATTAACAAGCC
TAGATATAAAAGGTTGTCGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-132)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

50

100

0.514
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.447

100

0.45
  pilA Vibrio cholerae strain A1552

41.447

100

0.45
  pilA Vibrio cholerae C6706

41.447

100

0.45
  pilA Pseudomonas aeruginosa PAK

41.333

100

0.443
  pilA Acinetobacter baumannii strain A118

40.58

98.571

0.4
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.028

100

0.386
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.235

97.143

0.371