Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   WCU25_RS24085 Genome accession   NZ_CP147542
Coordinates   5178319..5179539 (+) Length   406 a.a.
NCBI ID   WP_003112840.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain GN06816     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5173319..5184539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCU25_RS24065 (WCU25_24065) nadC 5174885..5175733 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  WCU25_RS24075 (WCU25_24075) pilA 5175932..5176384 (-) 453 WP_028681203.1 pilin Machinery gene
  WCU25_RS24080 (WCU25_24080) pilB 5176615..5178315 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WCU25_RS24085 (WCU25_24085) pilC 5178319..5179539 (+) 1221 WP_003112840.1 type 4a pilus biogenesis protein PilC Machinery gene
  WCU25_RS24090 (WCU25_24090) pilD 5179543..5180415 (+) 873 WP_003125204.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  WCU25_RS24095 (WCU25_24095) coaE 5180412..5181023 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  WCU25_RS24100 (WCU25_24100) yacG 5181020..5181220 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  WCU25_RS24105 (WCU25_24105) - 5181257..5181466 (-) 210 WP_003094660.1 hypothetical protein -
  WCU25_RS24110 (WCU25_24110) - 5181572..5182261 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  WCU25_RS24115 (WCU25_24115) - 5182258..5182728 (-) 471 WP_003125205.1 hypothetical protein -
  WCU25_RS24120 (WCU25_24120) - 5182725..5183150 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -
  WCU25_RS24125 (WCU25_24125) - 5183283..5183912 (+) 630 WP_003120948.1 DUF1780 domain-containing protein -
  WCU25_RS24130 (WCU25_24130) - 5183909..5184358 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44318.06 Da        Isoelectric Point: 9.8784

>NTDB_id=946875 WCU25_RS24085 WP_003112840.1 5178319..5179539(+) (pilC) [Pseudomonas aeruginosa strain GN06816]
MADKALKTSVFIWEGTDKKGAKVKGELTGQNPMLVKAHLRKQGINPLKVRKKGISLLGAGKKVKPMDIALFTRQMATMMG
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVSSGNSLANSLRKKPQYFDELYCNLVDAGEQSGALENLLDRVATYKEKT
ESLKAKIRKAMTYPIAVIIVALIVSAILLIKVVPQFQSVFQGFGAELPAFTQMVVNLSEFLQEWWLAVIVGVGAIGFTFK
ELHKRSKKFRDTLDRTILKLPIFGGIVYKSAVARYARTLSTTFAAGVPLVDALDSVSGATGNIVFKNAVSKIKQDVSTGM
QLNFSMRTTSVFPNMAIQMTAIGEESGSLDEMLSKVASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGNVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=946875 WCU25_RS24085 WP_003112840.1 5178319..5179539(+) (pilC) [Pseudomonas aeruginosa strain GN06816]
ATGGCGGACAAAGCGTTAAAAACCAGCGTTTTCATCTGGGAGGGCACCGACAAGAAAGGCGCCAAGGTCAAGGGCGAACT
GACCGGGCAGAATCCCATGCTGGTGAAGGCCCATCTGCGCAAGCAAGGCATCAATCCGCTCAAGGTACGCAAGAAAGGTA
TCTCCCTGCTGGGCGCAGGCAAGAAAGTGAAACCCATGGACATCGCCCTGTTCACCCGGCAGATGGCGACCATGATGGGC
GCTGGCGTTCCCCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGACAATCCCAACATGCGCAAGTTGGTGGATGA
GATCAAGCAGGAAGTGTCCTCGGGCAACAGCCTGGCCAACTCACTGAGAAAGAAACCACAGTACTTCGACGAACTCTATT
GCAACCTGGTCGATGCCGGCGAGCAATCGGGCGCCTTGGAAAACTTGCTGGATAGAGTCGCGACCTACAAGGAAAAAACG
GAATCGTTAAAAGCAAAGATCAGGAAGGCGATGACTTATCCTATCGCCGTCATCATCGTTGCCTTGATCGTCTCCGCCAT
TCTTCTGATCAAGGTCGTTCCGCAGTTCCAATCCGTATTCCAAGGTTTTGGCGCAGAGCTGCCTGCATTCACTCAAATGG
TCGTAAATTTATCCGAGTTCCTACAGGAGTGGTGGCTTGCCGTCATCGTTGGTGTGGGGGCGATTGGCTTTACCTTCAAG
GAACTGCATAAACGCTCGAAAAAATTCCGCGACACACTCGACAGAACGATCCTCAAACTTCCCATTTTCGGAGGCATCGT
CTACAAATCTGCGGTCGCCCGTTATGCACGGACCTTGTCCACGACCTTCGCCGCGGGTGTTCCCCTGGTCGATGCGCTCG
ACTCCGTCTCCGGAGCGACCGGCAATATCGTGTTCAAGAACGCGGTCAGCAAGATCAAGCAAGACGTTTCCACCGGCATG
CAGCTCAACTTCTCCATGCGCACCACCAGCGTCTTTCCCAACATGGCGATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTCGATGAGATGCTGAGCAAAGTCGCCAGCTACTACGAAGAGGAAGTCGACAACGCCGTGGACAACCTCACCACGC
TCATGGAACCGATGATCATGGCCGTTCTCGGCGTACTGGTTGGCGGTCTGATCGTGGCCATGTACCTTCCGATCTTCCAA
CTCGGCAACGTCGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

77.037

99.754

0.768
  pilC Acinetobacter baumannii D1279779

60.891

99.507

0.606
  pilC Acinetobacter baylyi ADP1

60.591

100

0.606
  pilC Legionella pneumophila strain ERS1305867

54.545

97.537

0.532
  pilG Neisseria meningitidis 44/76-A

45.658

99.261

0.453
  pilG Neisseria gonorrhoeae MS11

45.409

99.261

0.451
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

41.562

97.783

0.406
  pilC Thermus thermophilus HB27

37.157

98.768

0.367