Detailed information NTG0945366

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V9L13_RS08100
• Genome accession: NZ_CP146986
• Coordinates: 1820896..1822008 (-)
• Length: 370 a.a.
• NCBI ID: WP_338802127.1
• Uniprot ID: -
• Organism: Pseudomonas sp. RSB 5.4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1815896..1827008

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V9L13_RS08085 (V9L13_08085)	-	1816487..1817878 (+)	1392	WP_338802125.1	GntP family permease	-
V9L13_RS08090 (V9L13_08090)	hbdH	1817886..1818659 (+)	774	WP_027612662.1	3-hydroxybutyrate dehydrogenase	-
V9L13_RS08095 (V9L13_08095)	-	1818827..1820782 (+)	1956	WP_338802126.1	acetoacetate--CoA ligase	-
V9L13_RS08100 (V9L13_08100)	pilU	1820896..1822008 (-)	1113	WP_338802127.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V9L13_RS08105 (V9L13_08105)	-	1822142..1824691 (+)	2550	WP_338802128.1	PAS domain-containing protein	-
V9L13_RS08110 (V9L13_08110)	-	1824731..1825006 (+)	276	WP_003225447.1	peptidylprolyl isomerase	-
V9L13_RS08115 (V9L13_08115)	-	1825122..1826054 (-)	933	WP_338802129.1	sugar kinase	-

Sequence

Protein

• Length: 370 a.a.
• Molecular weight: 41124.99 Da
• Isoelectric Point: 6.6255

>NTDB_id=945366 V9L13_RS08100 WP_338802127.1 1820896..1822008(-) (pilU) [Pseudomonas sp. RSB 5.4]
MEIDALLLRLSSQHGSDLFLSTGAPPSARIDGVLTPFSERPFKPGEVEAIANSLMDAEQRREFDRELEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PVEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALTFADTGHLVLSTLHAHNANQALDRI
VNLFPEERRPQLLHALGNNLKAFVSQRLVRTVDGQRRAAVEVMLGTPTIADLIRRNELGELKGIMEKSGEVGMQTFDAAL
YGLVVEGVISEDEALKHADSQNNLRLRLKLHAEAGPAPAPTTSGDWGLMD

Nucleotide

• Length: 1113 bp

>NTDB_id=945366 V9L13_RS08100 WP_338802127.1 1820896..1822008(-) (pilU) [Pseudomonas sp. RSB 5.4]
ATGGAAATCGATGCACTGTTGCTCCGTCTGTCGAGCCAGCACGGCTCTGACCTGTTCCTGTCCACGGGAGCGCCACCCAG
TGCGCGCATCGATGGCGTGCTGACGCCGTTCAGCGAGCGGCCGTTCAAGCCCGGTGAGGTCGAGGCCATCGCTAACTCCT
TGATGGACGCCGAGCAGCGGCGCGAGTTCGATCGGGAACTGGAAATGAACCTGGCGATTTCCCGTACCGGTATCGGGCGT
TTTCGGGTGAACATCTTCAAACAACGCAATGACGTGTCGATCGTGATCCGCAACGTCAAACTCGATATCCCGCGTTTCGA
AGACCTCAAACTGCCGCCAGTGCTGCTGGAAACGGTGATGCTCAAACAGGGGCTGATCCTGTTCGTCGGTGCCACCGACT
CCGGCAAGTCCACCTCGCTGGCAGCGCTGATCGATCACCGTAACCGCCACAGCAGCGGGCACATCATCACCATTGAAGAC
CCGGTGGAGTATATCCATCGACACCAGCGCTCGATCATCAATCAACGCGAAGTCGGCGTCGATACCCGCAGTTTCCATGC
AGCGCTGAAAAACACTCTGCGTCAGGCCCCGGACGTGGTGCTGATCGGCGAAATCCGCGACCGCGAGACCATGGAACATG
CGCTGACATTCGCCGACACCGGGCACCTGGTGCTGTCGACGTTGCATGCGCACAACGCCAATCAGGCGCTGGACCGTATC
GTCAATCTGTTCCCCGAAGAGCGCCGACCGCAGTTGCTGCACGCGCTGGGCAACAATCTCAAGGCGTTTGTCTCGCAACG
GCTGGTGCGCACTGTGGACGGCCAGCGCCGGGCGGCGGTCGAAGTGATGCTCGGCACGCCCACCATCGCCGACCTGATCC
GCCGCAACGAGTTGGGCGAACTCAAGGGCATCATGGAAAAGTCAGGCGAGGTCGGCATGCAGACCTTCGACGCCGCGCTT
TACGGGCTGGTGGTGGAGGGCGTGATCAGCGAAGACGAAGCGCTCAAGCATGCGGATTCGCAGAACAACCTGCGCCTGCG
CCTGAAACTTCATGCCGAGGCGGGCCCGGCGCCCGCGCCCACTACATCAGGCGATTGGGGCCTGATGGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.62	95.946	0.543
pilU	Acinetobacter baylyi ADP1	53.221	96.486	0.514
pilU	Vibrio cholerae strain A1552	53.143	94.595	0.503
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.223	96.757	0.389
pilT	Pseudomonas stutzeri DSM 10701	40.116	92.973	0.373
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	39.884	93.514	0.373
pilT	Vibrio cholerae strain A1552	39.884	93.514	0.373
pilT	Pseudomonas aeruginosa PAK	39.535	92.973	0.368