Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WAC51_RS04815 Genome accession   NZ_CP146845
Coordinates   1051695..1052789 (+) Length   364 a.a.
NCBI ID   WP_043033789.1    Uniprot ID   A0A0X3QW38
Organism   Stenotrophomonas geniculata strain WXY53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1046695..1057789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAC51_RS04785 (WAC51_04785) soxR 1046761..1047195 (+) 435 WP_109814081.1 redox-sensitive transcriptional activator SoxR -
  WAC51_RS04790 (WAC51_04790) - 1047269..1047871 (-) 603 WP_010483701.1 short chain dehydrogenase -
  WAC51_RS04795 (WAC51_04795) - 1047970..1048845 (+) 876 WP_049434416.1 LysR family transcriptional regulator -
  WAC51_RS04800 (WAC51_04800) proC 1048905..1049726 (-) 822 WP_241882839.1 pyrroline-5-carboxylate reductase -
  WAC51_RS04805 (WAC51_04805) - 1049745..1050422 (-) 678 WP_010483695.1 YggS family pyridoxal phosphate-dependent enzyme -
  WAC51_RS04810 (WAC51_04810) pilT 1050510..1051547 (+) 1038 WP_006422551.1 type IV pilus twitching motility protein PilT Machinery gene
  WAC51_RS04815 (WAC51_04815) pilU 1051695..1052789 (+) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  WAC51_RS04820 (WAC51_04820) - 1052938..1053921 (+) 984 WP_057503304.1 aldo/keto reductase -
  WAC51_RS04825 (WAC51_04825) - 1053984..1054880 (-) 897 WP_338713344.1 LysR family transcriptional regulator -
  WAC51_RS04830 (WAC51_04830) - 1054974..1055186 (+) 213 WP_014646220.1 DUF4287 domain-containing protein -
  WAC51_RS04835 (WAC51_04835) - 1055282..1055731 (+) 450 WP_043398317.1 GNAT family N-acetyltransferase -
  WAC51_RS04840 (WAC51_04840) - 1055824..1056531 (+) 708 WP_029379885.1 YitT family protein -
  WAC51_RS04845 (WAC51_04845) - 1056605..1057147 (-) 543 WP_338713347.1 DNA-3-methyladenine glycosylase I -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40569.61 Da        Isoelectric Point: 6.7680

>NTDB_id=944658 WAC51_RS04815 WP_043033789.1 1051695..1052789(+) (pilU) [Stenotrophomonas geniculata strain WXY53]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=944658 WAC51_RS04815 WP_043033789.1 1051695..1052789(+) (pilU) [Stenotrophomonas geniculata strain WXY53]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
AATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGTCGTTTCCGCGTGAGCTGCTTCTACCAGCGC
AACCAGGTGGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACCGTGGAAGAGTTGAGTCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTTGTCGGCGCCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGA
TGATCGGCTACCGCAACCAGAATTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAA
GGCTGCATCATCACCCAGCGTGAGGTCGGCATCGATACCGACAGCTGGGAAGCCGCGTTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATCGGTGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAGACCGGCCACC
TGGTGCTGTGCACCCTGCATGCCAACAATGCCAACCAGGCGATGGACCGCATCGTCAACTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAACCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGATGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGTGAG
ATCAGCTACGAGGACGCGCTGCGCTATGCCGACTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGGGGCGA
CGCACGCACCCTGTCGCAGGGGCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X3QW38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363