NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	V7A40_RS00710	Genome accession	NZ_CP146750
Coordinates	145751..146695 (+)	Length	314 a.a.
NCBI ID	WP_265150863.1	Uniprot ID	A0AA43TCR2
Organism	Lactococcus garvieae strain L2
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 140751..151695

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V7A40_RS00695 (V7A40_00695)	-	142161..143615 (-)	1455	WP_341784649.1	GH25 family lysozyme	-
V7A40_RS00700 (V7A40_00700)	guaB	143784..145265 (+)	1482	WP_003133714.1	IMP dehydrogenase	-
V7A40_RS00705 (V7A40_00705)	rpsU	145388..145564 (+)	177	WP_003134539.1	30S ribosomal protein S21	-
V7A40_RS00710 (V7A40_00710)	comGA	145751..146695 (+)	945	WP_265150863.1	competence type IV pilus ATPase ComGA	Machinery gene
V7A40_RS00715 (V7A40_00715)	comGB	146634..147653 (+)	1020	WP_016171027.1	competence type IV pilus assembly protein ComGB	Machinery gene
V7A40_RS00720 (V7A40_00720)	comYC	147672..147977 (+)	306	WP_016171026.1	competence type IV pilus major pilin ComGC	Machinery gene
V7A40_RS00725 (V7A40_00725)	comGD	147952..148368 (+)	417	WP_014024177.1	competence type IV pilus minor pilin ComGD	Machinery gene
V7A40_RS00730 (V7A40_00730)	comGE	148400..148633 (+)	234	WP_341784650.1	competence type IV pilus minor pilin ComGE	-
V7A40_RS00735 (V7A40_00735)	comYF	148614..149054 (+)	441	WP_014024179.1	competence type IV pilus minor pilin ComGF	Machinery gene
V7A40_RS00740 (V7A40_00740)	-	149287..150132 (+)	846	WP_014024181.1	metal ABC transporter solute-binding protein, Zn/Mn family	-
V7A40_RS00745 (V7A40_00745)	-	150218..151087 (-)	870	WP_014570921.1	RluA family pseudouridine synthase	-

Sequence

Protein

Download Length: 314 a.a. Molecular weight: 35852.07 Da Isoelectric Point: 6.6641

                                >NTDB_id=944122 V7A40_RS00710 WP_265150863.1 145751..146695(+) (comGA) [Lactococcus garvieae strain L2]

MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLSGMNLGESRRVQLGACTYV

LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQNQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI

CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVISR

LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide

Download Length: 945 bp

                                >NTDB_id=944122 V7A40_RS00710 WP_265150863.1 145751..146695(+) (comGA) [Lactococcus garvieae strain L2]

ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTATTTCGTAGCATTAGA

AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCGATCTTGAACAAGGGCAAGCAC

TGATTGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT

TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT

GCATCATCAACAAAACCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGGAGAGGGCTTTACCTGT

TTTCCGGTCCCGTAGGCTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGGGAGCATTTTAAAAATCAACAAGTTATT

TGTATAGAAGATCCTGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA

TGCCTTGATTAAGCTCTCTCTTCGCCACAGGCCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAAACGGCAAAAG

CTGTTCTTCGCGCAAGTTTGACGGGATATACAGTGTTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTTCACGG

CTAAAGGAACTTGGATTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA

GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGATGAATGGAACGGCCAGATTGATCAGTTGGTTG

CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Lactococcus lactis subsp. cremoris KW2	59.554	100	0.596
comYA	Streptococcus gordonii str. Challis substr. CH1	53.651	100	0.538
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	53.355	99.682	0.532
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	53.035	99.682	0.529
comGA/cglA/cilD	Streptococcus pneumoniae D39	53.035	99.682	0.529
comGA/cglA/cilD	Streptococcus pneumoniae R6	53.035	99.682	0.529
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	53.035	99.682	0.529
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	52.077	99.682	0.519
comYA	Streptococcus mutans UA159	51.282	99.363	0.51
comYA	Streptococcus mutans UA140	51.282	99.363	0.51

Detailed information NTG0944122