NTDB

Overview

Name	comGA	Type	Machinery gene
Locus tag	V4331_RS00660	Genome accession	NZ_CP146743
Coordinates	137125..138069 (+)	Length	314 a.a.
NCBI ID	WP_341784488.1	Uniprot ID	-
Organism	Lactococcus formosensis subsp. formosensis strain L5
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Prophage	119557..136634	137125..138069	flank	491

Gene organization within MGE regions

Location: 119557..138069

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V4331_RS00580 (V4331_00580)	-	119557..120387 (+)	831	WP_096368812.1	glycosyltransferase	-
V4331_RS00585 (V4331_00585)	-	120486..121187 (+)	702	WP_096368811.1	glycosyltransferase family 2 protein	-
V4331_RS00590 (V4331_00590)	-	121195..121545 (+)	351	WP_017368733.1	DUF2304 domain-containing protein	-
V4331_RS00595 (V4331_00595)	-	121532..122476 (+)	945	WP_341784214.1	NAD-dependent epimerase/dehydratase family protein	-
V4331_RS00600 (V4331_00600)	-	122473..123906 (+)	1434	WP_341784215.1	lipopolysaccharide biosynthesis protein	-
V4331_RS00605 (V4331_00605)	-	123899..125038 (+)	1140	WP_185767487.1	glycosyltransferase family 4 protein	-
V4331_RS00610 (V4331_00610)	-	125035..125859 (+)	825	WP_096368807.1	phosphorylcholine transferase LicD	-
V4331_RS00615 (V4331_00615)	-	125880..126872 (+)	993	WP_096368806.1	glycosyltransferase	-
V4331_RS00620 (V4331_00620)	-	126874..127761 (+)	888	WP_270740735.1	glycosyltransferase family 2 protein	-
V4331_RS00625 (V4331_00625)	tagD	127786..128199 (+)	414	WP_096368804.1	glycerol-3-phosphate cytidylyltransferase	-
V4331_RS00630 (V4331_00630)	-	128284..129951 (+)	1668	WP_341784216.1	surface protein	-
V4331_RS00635 (V4331_00635)	-	129961..131328 (+)	1368	WP_096368802.1	DUF2142 domain-containing protein	-
V4331_RS00640 (V4331_00640)	-	131358..132962 (-)	1605	WP_341784217.1	GH25 family lysozyme	-
V4331_RS00645 (V4331_00645)	guaB	133136..134617 (+)	1482	WP_003133714.1	IMP dehydrogenase	-
V4331_RS00650 (V4331_00650)	-	134973..136634 (+)	1662	WP_341784218.1	S-layer homology domain-containing protein	-
V4331_RS00655 (V4331_00655)	rpsU	136763..136939 (+)	177	WP_003134539.1	30S ribosomal protein S21	-
V4331_RS00660 (V4331_00660)	comGA	137125..138069 (+)	945	WP_341784488.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

Download Length: 314 a.a. Molecular weight: 35777.90 Da Isoelectric Point: 6.1706

                                >NTDB_id=943990 V4331_RS00660 WP_341784488.1 137125..138069(+) (comGA) [Lactococcus formosensis subsp. formosensis strain L5]

MIQVLAKTLLQKAVDQMVHDLYLVALEGRYSLYFRTATERRFEEEVSLEQGQALIAHMKFLSGMNLGESRRVQLGACTYS

LDKGKQRLRLSTVGDFHGQESMVIRFLHHQQNQLYFWNSELFNTFMGGRGLYLFSGPVGSGKTSLMYKFAREYFKDQQVI

CIEDPVELVEAEFLQLQVNKVIGNDYDALINLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVVAR

LKELGLTDWELQSSLQRVIYQRLIAGKGLFVCEKEKFEEWQPDEWNGQIDQLVADGFISAATAAREKIEFSEAD

Nucleotide

Download Length: 945 bp

                                >NTDB_id=943990 V4331_RS00660 WP_341784488.1 137125..138069(+) (comGA) [Lactococcus formosensis subsp. formosensis strain L5]

ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCATGATCTTTACTTAGTAGCATTAGA

AGGGAGGTATTCTCTATATTTTCGTACAGCAACAGAAAGGAGATTTGAAGAAGAAGTATCCCTTGAACAAGGGCAAGCTC

TGATCGCTCATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGGGTACAGTTGGGGGCTTGCACTTATAGC

TTAGATAAAGGTAAGCAACGCTTACGTTTATCTACTGTCGGGGATTTTCATGGTCAAGAAAGTATGGTTATTCGATTCTT

GCATCATCAACAAAACCAACTTTATTTTTGGAACTCAGAGCTCTTTAACACTTTTATGGGTGGAAGAGGACTTTATCTGT

TTTCAGGACCTGTGGGGTCAGGTAAGACCAGCCTTATGTACAAGTTTGCAAGAGAGTATTTTAAGGATCAACAAGTTATT

TGTATAGAAGATCCTGTGGAGTTAGTGGAAGCTGAGTTTTTACAACTTCAAGTCAATAAAGTTATAGGGAATGATTACGA

TGCCTTGATTAATCTTTCTCTTCGCCATAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAGACGGCTAAGG

CTGTGCTTCGTGCAAGCTTGACTGGGTATACGGTGTTCTCCACTGTTCATGCACGCTCAATATCGGGTGTAGTTGCACGC

CTAAAAGAACTTGGATTAACTGATTGGGAACTACAATCCAGTCTTCAGCGAGTGATTTATCAGCGATTAATTGCAGGAAA

GGGATTATTTGTTTGTGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG

CAGATGGATTTATCTCAGCTGCTACAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGA	Lactococcus lactis subsp. cremoris KW2	59.236	100	0.592
comYA	Streptococcus gordonii str. Challis substr. CH1	52.532	100	0.529
comGA/cglA	Streptococcus sobrinus strain NIDR 6715-7	51.603	99.363	0.513
comGA/cglA/cilD	Streptococcus mitis NCTC 12261	51.438	99.682	0.513
comGA/cglA/cilD	Streptococcus pneumoniae R6	51.118	99.682	0.51
comGA/cglA/cilD	Streptococcus pneumoniae Rx1	51.118	99.682	0.51
comGA/cglA/cilD	Streptococcus pneumoniae D39	51.118	99.682	0.51
comGA/cglA/cilD	Streptococcus pneumoniae TIGR4	51.118	99.682	0.51
comYA	Streptococcus mutans UA159	50.962	99.363	0.506
comYA	Streptococcus mutans UA140	50.962	99.363	0.506

Detailed information NTG0943990