Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V6985_RS19135 Genome accession   NZ_CP146071
Coordinates   4138382..4140088 (+) Length   568 a.a.
NCBI ID   WP_058394588.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain GMT3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4133382..4145088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6985_RS19110 (V6985_19115) pdhR 4134513..4135277 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  V6985_RS19115 (V6985_19120) ampD 4135554..4136126 (-) 573 WP_169047635.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  V6985_RS19120 (V6985_19125) - 4136276..4136746 (+) 471 WP_256685968.1 TIGR02281 family clan AA aspartic protease -
  V6985_RS19125 (V6985_19130) nadC 4136751..4137608 (+) 858 WP_409011399.1 carboxylating nicotinate-nucleotide diphosphorylase -
  V6985_RS19130 (V6985_19135) tapA 4137944..4138378 (+) 435 WP_310012712.1 type IVa pilus major pilin TapA -
  V6985_RS19135 (V6985_19140) pilB 4138382..4140088 (+) 1707 WP_058394588.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  V6985_RS19140 (V6985_19145) pilC 4140217..4141458 (+) 1242 WP_059113460.1 type II secretion system F family protein Machinery gene
  V6985_RS19145 (V6985_19150) pilD 4141522..4142397 (+) 876 WP_256685974.1 A24 family peptidase Machinery gene
  V6985_RS19150 (V6985_19155) coaE 4142417..4143031 (+) 615 WP_256685976.1 dephospho-CoA kinase -
  V6985_RS19155 (V6985_19160) zapD 4143069..4143791 (+) 723 WP_256685978.1 cell division protein ZapD -
  V6985_RS19160 (V6985_19165) yacG 4143801..4143995 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62546.97 Da        Isoelectric Point: 5.4384

>NTDB_id=941557 V6985_RS19135 WP_058394588.1 4138382..4140088(+) (pilB) [Aeromonas salmonicida strain GMT3]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=941557 V6985_RS19135 WP_058394588.1 4138382..4140088(+) (pilB) [Aeromonas salmonicida strain GMT3]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTCTGCGAGCTGGAATATGGGGTACCTCTGCTAGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCTGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCAGATGACGATGCCCCCATCGTCAA
ATACATCAACAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCAGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCCGAGCGACGGCTGCCTCAGGATGGTCGCATCAAGCTCAAGCTCTCCCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCGCAATATCTGCGTGCCCTCTCCAAGCCTCAG
GGCATGATACTGGTGACAGGCCCGACCGGCTCTGGCAAAACGGTTTCTCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTCAACATCTCTACCGCCGAAGATCCAGTGGAGATCAACCTGCCCGGTGTCAACCAGGTGCAAGTCAATCCCAAGG
CGGGGCTGACCTTCGCCAGCGCCCTGCGATCCTTTCTTCGCCAGGATCCCGATGTGGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCTGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCTTCGGTAACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCGCCGGAGGTAGTGCCCGAGGCTGAACTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAGCCAGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATCATGCAAGGGGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCCTAGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.614

100

0.558
  pilB Acinetobacter baumannii D1279779

55.556

99.824

0.555
  pilB Legionella pneumophila strain ERS1305867

54.594

99.648

0.544
  pilB Vibrio cholerae strain A1552

54.513

99.472

0.542
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.341

92.958

0.375